Reviewed, UniProtKB/Swiss-Prot Q47AU8 (SYA_DECAR)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Daro_3303

Organism

Dechloromonas aromatica (strain RCB) [Complete proteome] [HAMAP]

Taxonomic identifier

159087 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Dechloromonas

Protein attributes

Sequence length	871 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 871	871	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q47AU8-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: D7C0FB8886B435F7	FASTA	871	94,564
	10 20 30 40 50 60 MKSSEIRQQF LDFFASKGHQ IVSSSSLVPH EDPTLLFTNA GMNQFKDVFL GFDKRPYSRA 70 80 90 100 110 120 TTSQKCVRAG GKHNDLENVG YTARHHTFFE MLGNFSFGDY FKRDAIKYAW ELLTEVYKLP 130 140 150 160 170 180 KDKLTVTVYA EDDEAYDIWT KEIGVPVERV IRIGDNKGAR YASDNFWMMG DTGPCGPCTE 190 200 210 220 230 240 IFYDHGEKYW GGPPGSPEED GDRFIEIWNN VFMQFNRDEA GVMHPLPKPS VDTGMGLERV 250 260 270 280 290 300 SAVLQGVHAN YEIDLFQALL KAAARETSDA DLDSPSLKVL ADHIRACSFL LADGVIPGNE 310 320 330 340 350 360 GRGYVLRRII RRAIRHGYKL GARAAFFHKM VPDLVAEMGM AYPELGQNQA KIVATLKQEE 370 380 390 400 410 420 DRFFETIEHG MAILEGELKS LGEGGVFNGD TAFKLHDTYG FPLDLTQDIC REHKITVDAA 430 440 450 460 470 480 AFDAAMARQK EQARAAGKFK MATNLEYDGP ATTFHGYAAL EYKANVLALY KDGIAVNQLN 490 500 510 520 530 540 EGEMGVVVLD DTPFYAESGG QVGDCGALQS VHGIFAVEDT QKIQATVFGH HGVVKTGTIT 550 560 570 580 590 600 VGNGVAAKVD VQARQRIMRN HSATHLLHKA LREVLGDHVQ QKGSQVDPDK TRFDFVHNQP 610 620 630 640 650 660 MTDEEIRRVE NIVNAEILAN VATETRVLPI AEAQKLGAMM LFGEKYGDDV RVLDIGSSRE 670 680 690 700 710 720 LCGGTHVSRT GDIGLFSITA EGGVAAGVRR VEAVTGDNAL AYMQDMESAL GGVAGTLKVL 730 740 750 760 770 780 PKDVHGRVLA VLDQVKKLER ELAALKGKLA SAQGDDMLSN AVDIKGAKVL AATLEGADVN 790 800 810 820 830 840 ALRETMDKLK DKLKSAAIVL ASVADGKVTL IAGVTADLTG KVKAGELVNL VAQQIGGKGG 850 860 870 GRPDMAQAGG TQPENLPAAL ASVAGWVEGK L

References

[1]

"Complete sequence of Dechloromonas aromatica RCB." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Di Bartolo G., Trong S., Kellar K., Schmutz J., Larimer F., Land M., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000089 Genomic DNA. Translation: AAZ48033.1.
RefSeq	YP_286503.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3566483.
GenomeReviews	Gene locus Daro_3303 in contig CP000089_GR.
KEGG	dar:Daro_3303.
NMPDR	fig|159087.4.peg.2547.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q47AU8.
Enzyme and pathway databases
BioCyc	DARO159087:DARO_3303-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q47AU8. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_DECAR

Accession

Primary (citable) accession number: Q47AU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	September 13, 2005
Last modified:	July 22, 2008
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)