Reviewed, UniProtKB/Swiss-Prot Q487H5 (SYA_COLP3)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	CPS_1042

Organism

Colwellia psychrerythraea (strain 34H / ATCC BAA-681) (Vibrio psychroerythus) [Complete proteome] [HAMAP]

Taxonomic identifier

167879 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Colwelliaceae › Colwellia

Protein attributes

Sequence length	876 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 876	876	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q487H5-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 22B85269B67F0D47	FASTA	876	96,237
	10 20 30 40 50 60 MIKSTAEVRQ AFLDFFATKQ HQIVKSSSLV PGNDATLLFT NAGMVPFKDV FLGAETRSYT 70 80 90 100 110 120 RATSAQRCVR AGGKHNDLEN VGYTARHHTF FEMMGNFSFG DYFKNDAISY AWEFLTGELG 130 140 150 160 170 180 LAKEKLLVTV YATDEEAFSY WRDEVGVPED KIIRIGDKSA NKKYESDNFW SMGDTGPCGP 190 200 210 220 230 240 CSEIFYDHGE DIFGGPPGSP DEDGDRFIEI WNIVFMQFNR QSDGRMDPLP NPSIDTGMGL 250 260 270 280 290 300 ERISAIMQNV HSNYEIDIFQ ALIKDTAALL DCSDLEHKSL RVIGDHIRSC SFLIVDGVVP 310 320 330 340 350 360 SNEGRGYVLR RIIRRAIRHG HKLEATGHFF HKLVASLIAQ MGEAYPELAQ QQAIIEKLLR 370 380 390 400 410 420 IEEEQFGRTL DRGMILLEDI LANLSGDIIK GDDVFKLYDT YGFPADLTAD IARERNLKID 430 440 450 460 470 480 KDGFDVAMKQ QRERAQQASQ FGTDYNQQLK SDQNTAFKGY DNDSYSATVV ELFNSQDQDP 490 500 510 520 530 540 VSQLNSGEQG IVILDHTPFY AESGGQVGDS GLLHLDGGVF EVTDTIKLGN AFAHRGTAHT 550 560 570 580 590 600 DVGLNRRVKA EINVERRAAI VKNHTATHLL HEALRKVLGE HVTQKGSLCD SDKLRFDFSH 610 620 630 640 650 660 FEGVTAQELH DVEQMVNNEI RRNHAKQTES MYIEEAKAKG AMALFGEKYD DEVRVVTLGD 670 680 690 700 710 720 FSIELCGGVH VNRTGDIGFL KIVSESGIAA GVRRIEAVTG TGALDFINQQ TASLTTIAAL 730 740 750 760 770 780 VKSDVTNASS KVELLISRSK QLEKEIGQLK QELAAQAGSD LVNNTIEING VKVLIADLGS 790 800 810 820 830 840 VESKALRGMV DELKNKMQSG VIMLATANGP KVGLIAGVTK DLVGRVKAGD LVNMVAQQVG 850 860 870 GKGGGRPDMA QAGGSQPENI TSALESVSAW LTEKLA

References

[1]

"The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses." Methe B.A., Nelson K.E., Deming J.W., Momen B., Melamud E., Zhang X., Moult J., Madupu R., Nelson W.C., Dodson R.J., Brinkac L.M., Daugherty S.C., Durkin A.S., DeBoy R.T., Kolonay J.F., Sullivan S.A., Zhou L., Davidsen T.M. , Wu M., Huston A.L., Lewis M., Weaver B., Weidman J.F., Khouri H., Utterback T.R., Feldblyum T.V., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 102:10913-10918(2005) [PubMed: 16043709] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000083 Genomic DNA. Translation: AAZ25326.1.
RefSeq	YP_267790.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3519393.
GenomeReviews	Gene locus CPS_1042 in contig CP000083_GR.
KEGG	cps:CPS_1042.
NMPDR	fig|167879.3.peg.1008.
TIGR	CPS_1042.
Phylogenomic databases
HOGENOM	Q487H5.
Enzyme and pathway databases
BioCyc	CPSY167879:CPS_1042-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q487H5. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_COLP3

Accession

Primary (citable) accession number: Q487H5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	September 13, 2005
Last modified:	July 22, 2008
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)