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Reviewed, UniProtKB/Swiss-Prot Q49AN0 (CRY2_HUMAN)

Last modified July 22, 2008. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cryptochrome-2
Gene names
Name: CRY2
Synonyms: KIAA0658
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length593 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK|NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK|NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL.

Cofactor

Binds 1 FAD per subunit By similarity.

Binds 1 5,10-methenyltetrahydrofolate non-covalently per subunit By similarity.

Subunit structure

Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with PER1 and PER2 C-terminal domains. Interaction with PER2 inhibits its ubiquitination and vice versa. Interacts with NFIL3 By similarity.

Subcellular location

Cytoplasm. Nucleus. Note= Translocated to the nucleus through interaction with other Clock proteins such as PER2 or ARNTL.

Tissue specificity

Expressed in all tissues examined including fetal brain, fibroblasts, heart, brain, placenta, lung, liver, sketal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Highest levels in heart and skeletal muscle.

Post-translational modification

Phosphorylation on Ser-266 by MAPK is important for the inhibition of CLOCK-ARNTL-mediated transcriptional activity. Phosphorylation by CSKNE requires interaction with PER1 or PER2.

Sequence similarities

Belongs to the DNA photolyase class-1 family.

Contains 1 DNA photolyase domain.

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Ontologies

Keywords

   Biological processBiological rhythms
Sensory transduction
Transcription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   LigandChromophore
FAD
Flavoprotein
Nucleotide-binding
   Molecular functionPhotoreceptor protein
Receptor
Repressor
   PTMPhosphoprotein

Gene Ontology (GO)

None. [Check GOA]

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 593593Cryptochrome-2

Regions

Domain22 – 189168DNA photolyase
Region230 – 507278FAD-binding
Region390 – 489100Required for inhibition of CLOCK-ARNTL-mediated transcription By similarity

Amino acid modifications

Modified residue2661Phosphoserine; by MAPK By similarity
Modified residue5581Phosphoserine; by MAPK By similarity

Experimental info

Sequence conflict4221S → G in AAH35161. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q49AN0-1 [UniParc].

Last modified November 28, 2006. Version 2.
Checksum: BF380424092BEBFB

FASTA59366,947
        10         20         30         40         50         60 
MAATVATAAA VAPAPAPGTD SASSVHWFRK GLRLHDNPAL LAAVRGARCV RCVYILDPWF 

        70         80         90        100        110        120 
AASSSVGINR WRFLLQSLED LDTSLRKLNS RLFVVRGQPA DVFPRLFKEW GVTRLTFEYD 

       130        140        150        160        170        180 
SEPFGKERDA AIMKMAKEAG VEVVTENSHT LYDLDRIIEL NGQKPPLTYK RFQAIISRME 

       190        200        210        220        230        240 
LPKKPVGLVT SQQMESCRAE IQENHDETYG VPSLEELGFP TEGLGPAVWQ GGETEALARL 

       250        260        270        280        290        300 
DKHLERKAWV ANYERPRMNA NSLLASPTGL SPYLRFGCLS CRLFYYRLWD LYKKVKRNST 

       310        320        330        340        350        360 
PPLSLFGQLL WREFFYTAAT NNPRFDRMEG NPICIQIPWD RNPEALAKWA EGKTGFPWID 

       370        380        390        400        410        420 
AIMTQLRQEG WIHHLARHAV ACFLTRGDLW VSWESGVRVF DELLLDADFS VNAGSWMWLS 

       430        440        450        460        470        480 
CSAFFQQFFH CYCPVGFGRR TDPSGDYIRR YLPKLKAFPS RYIYEPWNAP ESIQKAAKCI 

       490        500        510        520        530        540 
IGVDYPRPIV NHAETSRLNI ERMKQIYQQL SRYRGLCLLA SVPSCVEDLS HPVAEPSSSQ 

       550        560        570        580        590 
AGSMSSAGPR PLPSGPASPK RKLEAAEEPP GEELSKRARV AELPTPELPS KDA

« Hide

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References

« Hide 'large scale' references
[1]"Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins."
Hsu D.S., Zhao X., Zhao S., Kazantsev A., Wang R.-P., Todo T., Wei Y.-F., Sancar A.
Biochemistry 35:13871-13877(1996) [PubMed: 8909283] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, TISSUE SPECIFICITY.
Tissue: Fetal brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[3]"Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."
Ishikawa K., Nagase T., Suyama M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:169-176(1998) [PubMed: 9734811] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-593.
Tissue: Brain.
[4]"Characterization of photolyase/blue-light receptor homologs in mouse and human cells."
Kobayashi K., Kanno S., Smit B., van der Horst G.T.J., Takao M., Yasui A.
Nucleic Acids Res. 26:5086-5092(1998) [PubMed: 9801304] [Abstract]
Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[5]"Light-independent role of CRY1 and CRY2 in the mammalian circadian clock."
Griffin E.A. Jr., Staknis D., Weitz C.J.
Science 286:768-771(1999) [PubMed: 10531061] [Abstract]
Cited for: FUNCTION.

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Web resources

Wikipedia

Cryptochrome entry

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Cross-references

Sequence databases

BC035161 mRNA. Translation: AAH35161.1. Different initiation.
BC041814 mRNA. Translation: AAH41814.1.
AB014558 mRNA. Translation: BAA31633.1.
UniGeneHs.532491

3D structure databases

ModBaseSearch...

Genome annotation databases

EnsemblENSG00000121671. Homo sapiens. [Contig view]
KEGGhsa:1408.
NMPDRfig|9606.3.peg.5504.

Organism-specific databases

HGNCHGNC:2385. CRY2.
MIM603732. gene.
PharmGKBPA26905.
HUGESearch...
GenAtlasSearch...
GeneCardsSearch...
GeneLynxSearch...

Phylogenomic databases

HOVERGENQ49AN0.

Gene expression databases

ArrayExpressQ49AN0.
CleanExHS_CRY2.
GermOnlineENSG00000121671. Homo sapiens.

Family and domain databases

InterProIPR002081. Cryptochrome/DNA_photolyase_1.
IPR006050. DNA_photolyase_N.
IPR005101. Photolyase_FAD-bd/Cryptochr_C.
[Graphical view]
PfamPF00875. DNA_photolyase. 1 hit.
PF03441. FAD_binding_7. 1 hit.
[Graphical view]
ProDomPD004390. FAD_binding_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00394. DNA_PHOTOLYASES_1_1. False negative.
[Graphical view]
BLOCKSSearch...

Other Resources

SOURCESearch...
ProtoNetSearch...

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Entry information

Entry nameCRY2_HUMAN
AccessionPrimary (citable) accession number: Q49AN0
Secondary accession number(s): O75148, Q8IV71
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: July 22, 2008
This is version 26 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents