Reviewed, UniProtKB/Swiss-Prot Q4UN61 (GCP_RICFE)

Names and origin

Protein names

Recommended name:
    Probable O-sialoglycoprotein endopeptidase
      Short name=Glycoprotease
    EC=3.4.24.57

Gene names

Name:	gcp
Ordered Locus Names:	RF_0146

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated. HAMAP MF_01445
Cofactor	Zinc Probable.
Sequence similarities	Belongs to the peptidase M22 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	Probable O-sialoglycoprotein endopeptidase HAMAP MF_01445		PRO_0000303524
Sites
	Metal binding	112	1	Zinc Potential
	Metal binding	116	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4UN61-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 539FD6FA41886E00	FASTA	389	42,643
	10 20 30 40 50 60 MIKILGIESS CDDTAVSIIT ENREILSNII ISQNTEHAVF GGVVPEIAAR SHLSNLDKAL 70 80 90 100 110 120 KNVLKESNTK LTEISAIAAT SGPGLIGGVI VGSMFARSLS SAFKKPFIAI NHLEGHALTA 130 140 150 160 170 180 RLTDNIPYPY LLLLASGGHC QFVAVLGLGK YKILGSTIDD AIGEAFDKVA KMLNLAFPGG 190 200 210 220 230 240 PEIEKRAKLG DPHKYKFPKP IINSGNCNMS FSGLKTAVRT LIMSLKELND AVINDIAASF 250 260 270 280 290 300 QFTIGEILSS KVQDAIIAYE RVVNDYYENI DYTTQLNLKS FRQDEFKEEP AEHTKVREHR 310 320 330 340 350 360 LYPQNSLGSS FLNDAIVIAG GVAANKYLQE ILSNCAKTYG YQLIYPPIRL CTDNAAMIAY 370 380 AGLERYNNKL FTPLNFCPKA RWSLEDISK

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY60997.1.
RefSeq	YP_246162.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M22.001.
Genome annotation databases
GeneID	3400647.
GenomeReviews	Gene locus RF_0146 in contig CP000053_GR.
KEGG	rfe:RF_0146.
NMPDR	fig|315456.3.peg.665.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UN61.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0146-MON.
Family and domain databases
HAMAP	MF_01445. [Tree]
InterPro	IPR009180. Pept_M22_Osialgl. IPR000905. Peptidase_M22. [Graphical view]
PANTHER	PTHR11735. Pept_M22_Osialgl. 1 hit.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
ProDom	PD002367. Peptidase_M22. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00329. gcp. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_RICFE

Accession

Primary (citable) accession number: Q4UN61

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	July 5, 2005
Last modified:	November 25, 2008
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)