Reviewed, UniProtKB/Swiss-Prot Q4UN71 (DHSB_RICFE)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase iron-sulfur subunit
    EC=1.3.99.1

Gene names

Name:	sdhB
Ordered Locus Names:	RF_0136

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster. Binds 1 3Fe-4S cluster. Binds 1 4Fe-4S cluster.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and an hydrophobic anchor protein.
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 261	261	Succinate dehydrogenase iron-sulfur subunit
Regions
	Domain	28 – 119	92	2Fe-2S ferredoxin-type
	Domain	161 – 191	31	4Fe-4S ferredoxin-type
Sites
	Metal binding	80	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	85	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	88	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	100	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	171	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	174	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	177	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	181	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	228	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	234	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	238	1	Iron-sulfur 2 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UN71-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: A3BD2326E50BF9B5	FASTA	261	29,613
	10 20 30 40 50 60 MAELRLPPNS VVKKGKEHKE QEEMLKPRKV KIYRYDPDLD ENPTIDSFEI DLSKTGPMVL 70 80 90 100 110 120 DALIKIKNEI DSTLTFRRSC REGICGSCSM NIDGTNTLAC IKPIEEISGD IKIYPLPHMK 130 140 150 160 170 180 VVKDLVPDMS HFYAQYESIE PWLKTDSPTP SNSERLQSIK GREKLDGLYE CILCACCSTS 190 200 210 220 230 240 CPSYWWNGDK YLGPAILLQA YRWIADSRDD HTGERLEDLE DPSKLYRCHT IMNCTKTCPK 250 260 GLNPAKAIGK IKSLIAERHG V

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY60987.1.
RefSeq	YP_246152.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3400637.
GenomeReviews	Gene locus RF_0136 in contig CP000053_GR.
KEGG	rfe:RF_0136.
NMPDR	fig|315456.3.peg.655.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UN71.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0136-MON.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR001450. 4Fe4S_Fe_S_bd. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR012285. Fum_reductase_C. IPR004489. Succ_DHase/fum_Rdtase_Fe-S. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
Pfam	PF00111. Fer2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProDom	Q4UN71. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

DHSB_RICFE

Accession

Primary (citable) accession number: Q4UN71

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	July 5, 2005
Last modified:	September 2, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)