Reviewed, UniProtKB/Swiss-Prot Q4UNA0 (PARC_RICFE)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 4 subunit A
    EC=5.99.1.-
Alternative name(s):
    Topoisomerase IV subunit A

Gene names

Name:	parC
Ordered Locus Names:	RF_0107

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	737 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Topoisomerase IV is essential for chromosome segregation. It has relaxation of supercoiled DNA activity. Performs the decatenation events required during the replication of a circular DNA molecule By similarity.
Subunit structure	Composed of two subunits: parC and parE By similarity.
Subcellular location	Cell membrane; Peripheral membrane proteinBy similarity.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	DNA-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 737	737	DNA topoisomerase 4 subunit A		PRO_0000273114
Sites
	Active site	120	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UNA0-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 1DFAA1F754FC9201	FASTA	737	83,127
	10 20 30 40 50 60 MKEAKIENID FGNALSERYL AYALSTIMSR SLPDVRDGLK PVHRRLLYAM LQLRLEPNSG 70 80 90 100 110 120 YKKCARVVGD VIGKYHPHGD VAVYDTLVRL AQHFSLRYPL IDGQGNFGSI DGDNAAAMRY 130 140 150 160 170 180 TESRMTEICT LLMVDIEKDT VDFRPTYDDS DLEPVIMPAS FPNLLANGSE GIAVGMATNI 190 200 210 220 230 240 PPHNLHELCD ALVHLIDHPK AEISDMMNFI KGPDFPTGGI IIDKAELINA AYSTGRGSFR 250 260 270 280 290 300 MRSRWEKEEL SYGTYQIVVT EIPYQVQKSK LIEQIAILLK DKKIPLVSNI RDESTDIIRL 310 320 330 340 350 360 VIEPRDRGCD PQIVMESLFK LTNLESRIQL NMNVIGSNNV PRVMNILEVL QEFLAHRQNI 370 380 390 400 410 420 VTRRSTYLLN KIKHRLEILE GLRIAYLNLD EIIKIIREED EPKAIMMERF KLTEIQVEAI 430 440 450 460 470 480 LNTRLRSLRK LEEQEIINEH SNLQKQQAIL EEILNNPKEL WKIVKKEIKA VQTKFGLNTV 490 500 510 520 530 540 IGARRTSFEE VTLTNQVVDI TAFITKEPIT IICSKMGWVR SLKGHNTDLS TIKYKEGDAE 550 560 570 580 590 600 KFIIEAYTTD KILIVSSEGR FFTLLADNIS KGKGTGESIK LLVDIGNNDI TNILVHKSDQ 610 620 630 640 650 660 LLLLASSIGK GFLVNSNEVM AQTKTGKQIM NVPDGHTCIA CLPVNGDSIA CIGESRKLLV 670 680 690 700 710 720 FNIDEIPEMK KGQGVTLQKF KNAKLLDIKI FNKEDGLSWN NNGKVKLEKN IIAFLGKRGS 730 TGKLPPMGFH KNNRFSS

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY60958.1.
RefSeq	YP_246123.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3401076.
GenomeReviews	Gene locus RF_0107 in contig CP000053_GR.
KEGG	rfe:RF_0107.
NMPDR	fig|315456.3.peg.626.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UNA0.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0107-MON.
Family and domain databases
InterPro	IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR005742. TopoIV_A_Gneg. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 2 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
ProDom	PD000742. DNA_topoisoIV. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01062. parC_Gneg. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PARC_RICFE

Accession

Primary (citable) accession number: Q4UNA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	July 5, 2005
Last modified:	November 25, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)