Reviewed, UniProtKB/Swiss-Prot Q4UNA8 (TRMD_RICFE)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	RF_0099

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmPotential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 234	234	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060444
Regions
	Region	135 – 140	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	115	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UNA8-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: 03BC9F2B3DD24773	FASTA	234	26,461
	10 20 30 40 50 60 MFILHATILT VFPEMFPGTL GYSLAGQALH KNIWSYDVIN IRDFGLTKHK NVDDEAYGGG 70 80 90 100 110 120 DGLIMRPDVL GSSIDHALSL NPNAEIYYPS PRGRVFTQSF AKEMLKNKNL IFLCGRYEGI 130 140 150 160 170 180 DERVIEEYNV KEISVGDYIL SGGEIPTLTI LDCLIRLLPG VLMNQNTLSS ESFEEVGEFK 190 200 210 220 230 GGLECGLYTR PEIWRDRAVP SVLLSGNHRL INEWKKEQSR IITKLRRPEL LKDL

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY60950.1.
RefSeq	YP_246115.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3401068.
GenomeReviews	Gene locus RF_0099 in contig CP000053_GR.
KEGG	rfe:RF_0099.
NMPDR	fig|315456.3.peg.618.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UNA8.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0099-MON.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProDom	PD004978. tRNA_m1G_mtfrase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_RICFE

Accession

Primary (citable) accession number: Q4UNA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	July 5, 2005
Last modified:	November 25, 2008
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)