Reviewed, UniProtKB/Swiss-Prot Q4UND9 (SYC_RICFE)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	RF_0068

Organism

Rickettsia felis (Rickettsia azadi) [Complete proteome] [HAMAP]

Taxonomic identifier

42862 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00041
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Cysteinyl-tRNA synthetase HAMAP MF_00041		PRO_0000240950
Regions
	Motif	33 – 43	11	"HIGH" region HAMAP MF_00041
	Motif	274 – 278	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	31	1	Zinc By similarity
	Metal binding	216	1	Zinc By similarity
	Metal binding	241	1	Zinc By similarity
	Metal binding	245	1	Zinc By similarity
	Binding site	277	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4UND9-1 [UniParc]. Last modified July 5, 2005. Version 1. Checksum: F6E212C6C7060CF1	FASTA	459	53,183
	10 20 30 40 50 60 MQIQFHLYNT LSRTKEVFNP QDQTKVKMYV CGPTVYDNPH IGNSRSVVVY DLLYRIVIKI 70 80 90 100 110 120 FGEQSVKYVR NITDVDDKII DRAELLSITI NELTDKVTQE FHTNMAYLGC LLPSIEPKAT 130 140 150 160 170 180 EHIDVMIEII ERLIAKDHAY IADNHVYFDV LSAPNYTELS NRNLEEMFEG VRIENSKTKK 190 200 210 220 230 240 HPQDFVLWKP AKPNESANMN FKSPWGFGRP GWHIECSAMS YKYLGENFDI HGGGADLIFP 250 260 270 280 290 300 HHTNEIAQSR CAFPDSTYAK YWVHNGFLTV NGEKMSKSLG NFITVRNLMD KEISGEVVRL 310 320 330 340 350 360 FLLSSHYRRP LDYNDKAIED AKKTLDYWYR AIENINVQKI DLPHNFMQSL LDDMNTPLAV 370 380 390 400 410 420 KIINDYAKGV FISKTEEEKQ LNASAIITCA NFIGLMNKTP HEWFNSGVDE LYINELLNKR 430 440 450 LEAKKQKNWL LADQIRNQLL EEKIILEDKP DGTTIWRKE

References

[1]

"The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite." Ogata H., Renesto P., Audic S., Robert C., Blanc G., Fournier P.-E., Parinello H., Claverie J.-M., Raoult D. PLoS Biol. 3:1-12(2005) [PubMed: 15984913] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-1525 / URRWXCal2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000053 Genomic DNA. Translation: AAY60919.1.
RefSeq	YP_246084.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3400298.
GenomeReviews	Gene locus RF_0068 in contig CP000053_GR.
KEGG	rfe:RF_0068.
NMPDR	fig|315456.3.peg.550.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4UND9.
Enzyme and pathway databases
BioCyc	RFEL315456:RF_0068-MON.
Family and domain databases
HAMAP	MF_00041. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_RICFE

Accession

Primary (citable) accession number: Q4UND9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	July 5, 2005
Last modified:	November 4, 2008
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)