Reviewed, UniProtKB/Swiss-Prot Q50393 (EMBC_MYCSM)

Names and origin

Protein names

Recommended name:
    Probable arabinosyltransferase C
    EC=2.4.2.-

Gene names

Name:

embC

Organism

Mycobacterium smegmatis

Taxonomic identifier

1772 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	1074 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Arabinosyl transferase responsible for the polymerization of arabinose into the arabinan of arabinogalactan.
Subcellular location	Cell membrane; Multi-pass membrane proteinProbable.
Miscellaneous	This is one of the targets of the anti-tuberculosis drug ethambutol [(S,S')-2,2'-(ethylenediimino)di-1-butanol; EMB]. EMB is a first-line drug used to treat tuberculosis. EMB inhibits the transfer of arabinogalactan into the cell wall.
Sequence similarities	Belongs to the emb family.

Ontologies

Keywords
Biological process	Antibiotic resistance Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring pentosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1074	1074	Probable arabinosyltransferase C		PRO_0000220572
Regions
	Transmembrane	15 – 37	23	Potential
	Transmembrane	214 – 236	23	Potential
	Transmembrane	251 – 273	23	Potential
	Transmembrane	415 – 437	23	Potential
	Transmembrane	452 – 474	23	Potential
	Transmembrane	516 – 538	23	Potential
	Transmembrane	573 – 595	23	Potential
	Transmembrane	608 – 630	23	Potential
	Transmembrane	645 – 667	23	Potential
	Transmembrane	684 – 706	23	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q50393-1 [UniParc]. Last modified July 1, 1997. Version 2. Checksum: 68DB19FE8839C301	FASTA	1,074	115,392
	10 20 30 40 50 60 MTGPHAAGGS NHRTARLVAI IAGLLGTLMA IATPLLPVEQ TTAELNWPQN GVWQSVDAPL 70 80 90 100 110 120 IGYVATDLTV TVPCQAAAGL VGPENRNRSV LLSTVPKQAP KAIDRGLLIE RINNDLTVIV 130 140 150 160 170 180 RNTPVVSAPL EQVLSPDCRY LTFTAHADKV TGEFVGLTQG PDDDDPGEAV RGERSGYDFR 190 200 210 220 230 240 PQIVGVFTDL SGPAPEGLQL SATIDTRYST SPTLLKLLAM IVGVAMTVIA LGALHVLDCA 250 260 270 280 290 300 DGRRHKRFLP SRWWSMTPLD GLVSAMLVWW HFVGANTADD GYILTMARVS EHAGYMANYY 310 320 330 340 350 360 RWFGTPESPF GWYYDLLALW AHVSTASVWM RFPTLLMGLA CWWVISREVI PRLGAAAKHS 370 380 390 400 410 420 RAAAWTAAGL FLAFWLPLNN GLRPEPIIAL GILLTWCSVE RGVATSRLLP VAVAIIIGAL 430 440 450 460 470 480 TLFSGPTGIA AVGALLVAIG PLKTIVAAHV SRFGYWALLA PIAAAGTVTI FLIFRDQTLA 490 500 510 520 530 540 AELQASSFKS AVGPSLAWFD EHIRYSRLFT TSPDGSVARR FAVLTLLLAL AVSIAMTLRK 550 560 570 580 590 600 GRIPGTALGP SRRIIGITII SFLAMMFTPT KWTHQFGVFA GLAGCLGALA AVAVTTTAMK 610 620 630 640 650 660 SRRNRTVFGA AVLFVTALSF ATVNGWWYVS NFGVPWSNSF PEFKFGFTTM LLGLSVLALL 670 680 690 700 710 720 VAAWFHFSGR DVSPDRPQRR WQRLLVAPLA VATWALVIFE VVSLTLGMIN QYPAWSVGRS 730 740 750 760 770 780 NLNALTGKTC GLANDVLVEQ NANAGMLTPI GEPAGQALGA VTSLGFGPNG IPSDVSADPV 790 800 810 820 830 840 MEQPGTDNFA DSDSGVVTGT EVGTEGGTTA AAGINGSRAR LPYGLNPATT PVLGSWRSGT 850 860 870 880 890 900 QQPAVLRSAW YRLPDRDQAG PLLVVSAAGR FDQGEVEVQW ATDEQAAANE PGGSITFGDV 910 920 930 940 950 960 GAAPAWRNLR APLSSIPPEA TQIRLVASDD DLAPQHWIAL TPPRIPELRT LQEVVGSSDP 970 980 990 1000 1010 1020 VMLDWLVGLA FPCQRPFDHR YGVVEVPKWR ILPDRFGAEA NSPVMDYLGG GPLGITELLL 1030 1040 1050 1060 1070 RPSSVPTYLK DDWYRDWGSL QRLTPWYPDA QPARLDLGTA TRSGWWSPAP LRLS

References

[1]

"The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol." Telenti A., Philipp W.J., Sreevatsan S., Bernasconi C., Stockbauer K.E., Wieles B., Musser J.M., Jacobs W.R. Jr. Nat. Med. 3:567-570(1997) [PubMed: 9142129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: imm30.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U46844 Genomic DNA. Translation: AAC45271.1.
PIR	T45094.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR007680. Arab_transf. [Graphical view]
Pfam	PF04602. Arabinose_trans. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EMBC_MYCSM

Accession

Primary (citable) accession number: Q50393

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	July 1, 1997
Last modified:	November 25, 2008
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)