Reviewed, UniProtKB/Swiss-Prot Q54AX5 (LRRA_DICDI)

Names and origin

Protein names

Recommended name:
    Leucine-rich repeat protein lrrA

Gene names

Name:	lrrA
ORF Names:	DDB_0215361

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in cytoskeleton remodeling, which is needed for normal chemotactic aggregation and efficient cell sorting during multicellular morphogenesis.
Subcellular location	Cytoplasm.
Developmental stage	Expressed during vegetative growth and up-regulated at early development, with a peak at 6-9 hours of the aggregation stage and then declined abruptly from 16 hours of development.
Miscellaneous	Loss of function mutant lrrA- is defective in the regulation of F-actin organization.
Sequence similarities	Contains 20 LRR (leucine-rich) repeats.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Leucine-rich repeat Repeat
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aggregation involved in sorocarp development Ref.1 Inferred from mutant phenotype. Source: dictyBase cell-cell adhesion Ref.1 Inferred from mutant phenotype. Source: dictyBase cell-substrate adhesion Ref.1 Inferred from mutant phenotype. Source: dictyBase chemotaxis to cAMP Ref.1 Inferred from mutant phenotype. Source: dictyBase chemotaxis to folate Ref.1 Inferred from mutant phenotype. Source: dictyBase culmination during sorocarp development Ref.1 Inferred from mutant phenotype. Source: dictyBase establishment or maintenance of cell polarity Ref.1 Inferred from mutant phenotype. Source: dictyBase regulation of actin filament polymerization Ref.1 Inferred from mutant phenotype. Source: dictyBase
Cellular component	phagocytic vesicle Ref.4 Inferred from direct assay. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 510	510	Leucine-rich repeat protein lrrA
Regions
	Repeat	14 – 34	21	LRR 1
	Repeat	35 – 59	25	LRR 2
	Repeat	60 – 82	23	LRR 3
	Repeat	84 – 106	23	LRR 4
	Repeat	107 – 130	24	LRR 5
	Repeat	132 – 152	21	LRR 6
	Repeat	153 – 176	24	LRR 7
	Repeat	177 – 200	24	LRR 8
	Repeat	202 – 222	21	LRR 9
	Repeat	224 – 245	22	LRR 10
	Repeat	246 – 270	25	LRR 11
	Repeat	272 – 292	21	LRR 12
	Repeat	293 – 315	23	LRR 13
	Repeat	316 – 340	25	LRR 14
	Repeat	341 – 363	23	LRR 15
	Repeat	365 – 386	22	LRR 16
	Repeat	387 – 408	22	LRR 17
	Repeat	410 – 432	23	LRR 18
	Repeat	433 – 458	26	LRR 19
	Repeat	460 – 478	19	LRR 20

Sequences

	Sequence		Length	Mass (Da)
	Q54AX5-1 [UniParc]. Last modified May 2, 2006. Version 1. Checksum: B62713D7AF79FF33	FASTA	510	56,807
	10 20 30 40 50 60 MGGNLSSELK STKYRKREIV DLRKMNIDKL PPTIGALQCK ELLLSENDLI TIPEEIGKLS 70 80 90 100 110 120 KVEIIDFAKN RINYIPPEIG SLATLKQLFL SNNKLFYTPI TPNIGALKNL TRLDLSSNQL 130 140 150 160 170 180 DDLPVEISNC EALEYLDISD NQLQSFPLEF GKLYNLQVFN CSKNSLKSLP SEISGWVKLE 190 200 210 220 230 240 ELNVSNNQLA FLPNQICLLG LLSTLNVGFN KLQQLPEELS SMVSLTNLDL KVNPPLQYVP 250 260 270 280 290 300 QLSNLRQLKI LSIRNLQITH LPLGLGLLSE LIELDIRDNP QLKEIPYDIA TLINLQKLDL 310 320 330 340 350 360 FGNNMRIVPR EVGNLINLQT LDLRQNKLTI DNIPSEIGKL VNLKKLLLSN NLLIALPPEI 370 380 390 400 410 420 ASMKALKEFE ASNNQLQAIP TEIGELSGLT KINLSGNKLT SIPASFGNLS ELQICDLKSN 430 440 450 460 470 480 EIAELPTTLD GLKSCTKIDL SHNMLTELPW EFGDLIGLTI LDVGHNPLTI PPNPIVMKGT 490 500 510 ESIIQWLKKN EKEGRKGKVS GLGIQQDNEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"LrrA, a novel leucine-rich repeat protein involved in cytoskeleton remodeling, is required for multicellular morphogenesis in Dictyostelium discoideum." Liu C.-I., Cheng T.-L., Chen S.-Z., Huang Y.-C., Chang W.-T. Dev. Biol. 285:238-251(2005) [PubMed: 16051212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE. Strain: AX3-1.
[2]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[4]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF200466 mRNA. Translation: AAF66828.1. AAFI02000006 Genomic DNA. Translation: EAL60413.1. AAFI02000226 Genomic DNA. Translation: EAT01372.1.
RefSeq	XP_001733086.1. XP_628849.1.
3D structure databases
HSSP	HSSP built from PDB template 1M0Z based on UniProtKB P07359.
ModBase	Search...
Genome annotation databases
GeneID	3385490. 5860590.
KEGG	ddi:DDB_0215361. ddi:DDBDRAFT_0215064.
Organism-specific databases
dictyBase	DDB0215361. lrrA.
Family and domain databases
InterPro	IPR001611. LRR. IPR003591. LRR_sub-typ. [Graphical view]
Pfam	PF00560. LRR_1. 13 hits. [Graphical view]
PRINTS	PR00019. LEURICHRPT.
SMART	SM00369. LRR_TYP. 1 hit. [Graphical view]
ProDom	Q54AX5. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

LRRA_DICDI

Accession

Primary (citable) accession number: Q54AX5
Secondary accession number(s): Q9NIR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 2, 2006
Last modified:	September 23, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)