Reviewed, UniProtKB/Swiss-Prot Q54BC8 (PSB5_DICDI)

Names and origin

Protein names

Recommended name:
    Proteasome subunit beta type-5
    EC=3.4.25.1

Gene names

Name:	psmB5
ORF Names:	DDB_0232959

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity By similarity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.
Subcellular location	CytoplasmBy similarity. NucleusBy similarity.
Sequence similarities	Belongs to the peptidase T1B family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Ref.2 Inferred from direct assay. Source: dictyBase
Cellular component	cytoplasm Ref.2 Inferred from physical interaction. Source: dictyBase nucleus Ref.2 Inferred from physical interaction. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Propeptide	1 – 62	62	By similarity
	Chain	63 – 272	210	Proteasome subunit beta type-5
Sites
	Active site	63	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54BC8-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 9C6D656C754404C4	FASTA	272	30,280
	10 20 30 40 50 60 MINIDFDNIE QSQPTLYDTL ASDVKFLNTP HFELPPNANP GDFLRETFDH PSAPKALEFA 70 80 90 100 110 120 HGTTTLAFVY GGGIIVSVDS KSTQGPYVAS RSVKKVIEIT PTLLGTMAGG AADCSFWERE 130 140 150 160 170 180 LGRRCRLYEL RNKELISVAA ASKILANIVY SYKGYGLSMG TMITGWDKTG PQLYYVDNDG 190 200 210 220 230 240 TRLHGQRFSC GSGSTYAYGV LDTGFKWDMN DEEAYELGRR AVYHATHRDA YSGGAINVYH 250 260 270 VQKDGWKKIS SDDCFKLYQK YYNIVPINKP AN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Proteasomes from Dictyostelium discoideum: characterization of structure and function." Schauer T.M., Nesper M., Kehl M., Lottspeich F., Mueller-Taubenberger A., Gerisch G., Baumeister W. J. Struct. Biol. 111:135-147(1993) [PubMed: 8130037] [Abstract] Cited for: IDENTIFICATION IN THE PROTEASOME COMPLEX.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000219 Genomic DNA. Translation: EAL60569.1.
RefSeq	XP_628966.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	T01.012.
Genome annotation databases
GeneID	3385635.
KEGG	ddi:DDB_0232959.
Organism-specific databases
dictyBase	DDB0232959. psmB5.
Family and domain databases
InterPro	IPR000243. Pept_T1A_subB. IPR001353. Proteasome_A_B_su. [Graphical view]
Pfam	PF00227. Proteasome. 1 hit. [Graphical view]
PRINTS	PR00141. PROTEASOME.
PROSITE	PS00854. PROTEASOME_B. False negative. [Graphical view]
ProDom	Q54BC8. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

PSB5_DICDI

Accession

Primary (citable) accession number: Q54BC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	July 22, 2008
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)