Reviewed, UniProtKB/Swiss-Prot Q54BM1 (RPA2_DICDI)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase I subunit rpa2
    EC=2.7.7.6
Alternative name(s):
    DNA-directed RNA polymerase I polypeptide 2
      Short name(s)=RNA polymerase I subunit 2

Gene names

Name:	polr1b
Synonyms:	rpa2
ORF Names:	DDB_0216278

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1130 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits.
Subcellular location	Nucleus › nucleolusBy similarity.
Miscellaneous	Three distinct zinc-containing RNA polymerases are found in eukaryotic nuclei: polymerase I for the ribosomal RNA precursor, polymerase II for the mRNA precursor, and polymerase III for 5S and tRNA genes.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 1130	1130	DNA-directed RNA polymerase I subunit rpa2
Regions
	Zinc finger	1070 – 1096	27	C4-type Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54BM1-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: A5DA000C3AEFBA9B	FASTA	1,130	128,285
	10 20 30 40 50 60 MSEELKSLIQ PHIDSFDFFT DHGIDLVLKD SEPLYFEHNS TQYKIRFTDL KLSRPTRNIV 70 80 90 100 110 120 EKEIKLYPNE SRETGTTYNA PFKGAIGLYE VNEMDEMDDG TEVDYNKQEP THTFEVDLGR 130 140 150 160 170 180 LPIMVKSRYC HLNGMSPEKL IESREEELEQ GGYFIVNGNE KVVRMLVMTK ANHPVALHRK 190 200 210 220 230 240 AWTNRGPGYT KNGITIRCVR PDRSSITNNL HYINDGQTTL RFLYRRQEFF LPATLLLKAL 250 260 270 280 290 300 KDTTEKEIYE NIVQGDNENT FLTDRVELSL REQKINNINT QEEVLDYIGS RFRARTQFPP 310 320 330 340 350 360 SFSNIKIGRW FINQFVFVHL PNYNDKYNLL ILMIQKLYAM VGGKCGTDNI DSPAFQEVLL 370 380 390 400 410 420 AGHIYGMILK EKLTEYLEST KSFVLKSFED KKKSGDKLPD ALKKVLDKNF KIADRFSYFL 430 440 450 460 470 480 ATGNLRSSSG IDLQQTSGFC VIADKLNFLR FISHFRSIHR GAFFATMKTT AIRKLMPESW 490 500 510 520 530 540 GFFCPVHTPD GAPCGLLNHL SSNCLVTVDA AQDPTVQKAQ TLLPSFLAAH GMLPIDMVSV 550 560 570 580 590 600 YQHQYLTITL DGRVLGKIEP KAGEEMVKML RYLRSMGNEP SIPKTMEISY APPTNVENGQ 610 620 630 640 650 660 YSGISLFTTG ARFMRPVVNL TSGQNELIGP QEQLYMEIAV VPHEVIKGVT THIETSPTAM 670 680 690 700 710 720 FSLLANLTPF SDYNQSPRNM YQCQMAKQTM GTPLHSYPFR TDNKLYKVQN VQKPIVHTKN 730 740 750 760 770 780 QFKFRCNDYP HGCNAVIAVI SNTGYDMEDA MIINKSAYER GFGHGSVYKN EYIDLDQGLS 790 800 810 820 830 840 RFEQKKTFFG RPNTVVDELD KFIDTDGLPF VGRLIKPGEP YYTYIRPSDG RQVTKDYKGK 850 860 870 880 890 900 EEAYIEDVRI IFGPNGATRD PSKINNICVK LRFNRNPVIG DKFSSRHGQK GVLSQLWPEI 910 920 930 940 950 960 NMPFTESGMK PDVIINPNAF PSRMTIGMLV EILAGKAGAI HGKFQDATAF QFDENNTAID 970 980 990 1000 1010 1020 FFGEQLAKAG FNRFGNEQMY SGTTGKEFEC EIFFGVCYYQ RLRHMVKDKY QVRALGKVNA 1030 1040 1050 1060 1070 1080 LTRQPIKGRK VGGGIRFGEM ERDSLLAHGA SFCLNDRLMK SSDFAKIKVC KLCGSTLTIY 1090 1100 1110 1120 1130 SKKDYSNQTV SECKSCKSSD SIATISIPYV FTYLVAELAA VNITMKLQVS

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000218 Genomic DNA. Translation: EAL60592.1.
RefSeq	XP_629008.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3385677.
KEGG	ddi:DDB_0216278.
Organism-specific databases
dictyBase	DDB0216278. polr1b.
Family and domain databases
InterPro	IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR009674. RNA_pol_Rpa2-specific. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. [Graphical view]
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF06883. RNA_pol_Rpa2_4. 1 hit. PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProDom	Q54BM1. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

RPA2_DICDI

Accession

Primary (citable) accession number: Q54BM1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	July 22, 2008
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)