Reviewed, UniProtKB/Swiss-Prot Q54GJ7 (HOT_DICDI)

Names and origin

Protein names

Recommended name:
    Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial
      Short name=HOT
    EC=1.1.99.24

Gene names

Name:	adhfe1
ORF Names:	DDB_0252839

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	547 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG By similarity.
Catalytic activity	(S)-3-hydroxybutanoate + 2-oxoglutarate = acetoacetate + (R)-2-hydroxyglutarate. 2-oxoglutaric acid + 4-hydroxybutanoic acid = (R)-2-hydroxyglutaric acid + succinic semialdehyde.
Subcellular location	MitochondrionBy similarity.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	molecular hydrogen transport Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	hydroxyacid-oxoacid transhydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 547		Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial

Sequences

	Sequence		Length	Mass (Da)
	Q54GJ7-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: B03F1DF8B3B4DFC4	FASTA	547	59,743
	10 20 30 40 50 60 MTIIITKIAD KIIKSNRVKN TLQDIHIHSS NCGCHGSFKS NSVMNQTFSS FSSLTTEKNE 70 80 90 100 110 120 QQQQQLFKQF SNSKPIPMGR KVELDTSKVI DSVYFGNTEN NNYRTNKGNT DYAFEASANN 130 140 150 160 170 180 IRFGSGVTYE VGYDLLDMNC RNVIVFTDNN LLKLVDTDKE SAVAKCLYSL EKCGIKYTIY 190 200 210 220 230 240 SDVSIEPTDT SFKDAISVMG KGRYDGVVAV GGGSVMDTAK AANLYNSYPP ADNDFLAYIN 250 260 270 280 290 300 PPIGKGLLVP GPLKRPLIAI PTTCGTASET TGVCILDIKL GDGLSAKTGI ASRHLKPILG 310 320 330 340 350 360 LVDPDNLLTL PSNVAISSGF DQLCHALESF TAIPFNQRSP RPLAPNQRPS YQGANPVSDV 370 380 390 400 410 420 WSLRSLEMLC KNIHRFVLNP NDDYARSQMM LAASYAGLGF GNSGVHACHG MSYSISSMVK 430 440 450 460 470 480 DYKPEGYYGL KKNLIPHGQS VILSAPAVFK FTAPSNPERH LLLAKIMGAD ISNASESDAG 490 500 510 520 530 540 VLLSNQIVKL MKLLNVPNGL QALGYKESDI DSLVKGTLPQ HRVTKLMPKQ ATYDDLYKLF KDSMTIY

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000153 Genomic DNA. Translation: EAL62385.1.
RefSeq	XP_635889.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3387462.
KEGG	ddi:DDBDRAFT_0219524.
Organism-specific databases
dictyBase	DDB0252839. adhfe1.
Family and domain databases
InterPro	IPR001670. Fe_AlcDHase. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. False negative. PS00060. ADH_IRON_2. False negative. [Graphical view]
ProDom	Q54GJ7. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

HOT_DICDI

Accession

Primary (citable) accession number: Q54GJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	May 24, 2005
Last modified:	July 22, 2008
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)