Reviewed, UniProtKB/Swiss-Prot Q54P79 (4CL3_DICDI)

Names and origin

Protein names

Recommended name:
    Probable 4-coumarate--CoA ligase 3
      Short name=4CL 3
    EC=6.2.1.12
Alternative name(s):
    4-coumaroyl-CoA synthase 3

Gene names

Name:	4cl3
ORF Names:	DDB_G0284743

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
Pathway	Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from 4-coumaric acid: step 1/2.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.
Sequence caution	The sequence EAL65024.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-coumarate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 551	551	Probable 4-coumarate--CoA ligase 3		PRO_0000327703

Sequences

	Sequence		Length	Mass (Da)
	Q54P79-1 [UniParc]. Last modified April 8, 2008. Version 2. Checksum: 29FD8808DCB9615B	FASTA	551	61,163
	10 20 30 40 50 60 MIRIINGQTY FTSKYPNIII PEKPIPHLIL KHIRYKPDQV LLVDGLTFKE YSSHFVADTI 70 80 90 100 110 120 EKVACGLNKL NIKKGDVLGV ILPNLPEYVP IFHGTLLMGG ITSLVNPDYT IEELSHTLAT 130 140 150 160 170 180 VSPRYLAVTL AVYEKIKNDL KRVFPSVEKV ILVDIAGQTL KEIDQLTLSS DGIVMSFNQL 190 200 210 220 230 240 TNNNGKDYPI VRIDLTKDTA IIPFSSGTTG LFKGVCLSHY NLVSNTYQTQ TIETSTYKKN 250 260 270 280 290 300 DSVIGVLPFF HSFGLMLHIM LMVKQGYRIV TLPKFEPVRF LELIKKYKVA MSFIVPPIAI 310 320 330 340 350 360 MFAKSPIVDK FDLSSLRTLF CGAAPLGSEI EDLIKERFKG RLVIKQGYGA TELSPCCFVT 370 380 390 400 410 420 PNGLVKSGSS GTLLPNLLAK IISSETGENL GMGEKGEICI KGPNVMLGYY NNEKATNEVI 430 440 450 460 470 480 DKDGFLKTGD IGYVDEDGYF FIIDRSKELI KCKGFQVPPA ELEALLLSHP KVADACVVGL 490 500 510 520 530 540 SKGDMGEVPR GFVVIKQNES LTEKELLDWA HPKIANYKHF RGGIFFIPAI PKSATGKLLR 550 KNLKDFNNLK L

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000071 Genomic DNA. Translation: EAL65024.1. Sequence problems.
RefSeq	XP_638379.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3389988.
KEGG	ddi:DDBDRAFT_0186164.
Organism-specific databases
dictyBase	DDB_G0284743. 4cl3.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PRINTS	PR00154. AMPBINDING.
PROSITE	PS00455. AMP_BINDING. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

4CL3_DICDI

Accession

Primary (citable) accession number: Q54P79

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	April 8, 2008
Last modified:	November 25, 2008
This is version 24 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)