Reviewed, UniProtKB/Swiss-Prot Q54Y20 (SYA_DICDI)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
ORF Names:	DDB_G0277823

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	946 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro alanine-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 946	946	Alanyl-tRNA synthetase		PRO_0000315603
Experimental info
	Sequence conflict	18	1	C → Y in AAF05592. Ref.1
	Sequence conflict	340	1	A → T in AAF05592. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q54Y20-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: B5A076C7CBF441FB	FASTA	946	106,281
	10 20 30 40 50 60 MDVNQIRKTF IDFFREKCEH TFVPSSAVIP HDDPTLLFAN AGMNQFKPIF LGQVNPKSEQ 70 80 90 100 110 120 AKLKRAVNSQ KCIRAGGKHN DLDDVGKDTY HHTFFEMLGN WSFGNYFKKE AITWAWELLT 130 140 150 160 170 180 EVYKLDKERL YVTYFRGDPE KGLEADLEAK NLWLQYLPEE RVLPFGMKEN FWEMGDQGPC 190 200 210 220 230 240 GPCSEIHYDK VEGRDGASFV NADDPTLIEI WNLVFIQYNR EADKSLRPLP QKHVDTGMGL 250 260 270 280 290 300 ERLTSIIQKV PTNYDTDVFM PIFAAIQEVT GYPEPYGGKV GAEDTQQVDM AYRVIADHIR 310 320 330 340 350 360 TLTFSIADGA APSVDGRGQV LRRILRRAVR YGKQKLNAPA GFFSKLVDVV IANFGEFFPE 370 380 390 400 410 420 LRKKPEHIKM VLTREEDMFN KTLEKGIVEF EKMIKKSVNN TLSAENAYFL STCYGFPIDL 430 440 450 460 470 480 TTIMAEEKNY KVDIKGYEGL CEAQSEIDRK RQKEKKVELT LGAEANAWLK NNDIKPTDDS 490 500 510 520 530 540 FKYKQHEIQS VIKAIWNGKE FVDTAPKGTL IGVVLENSNF YAEQGGQIYD IGQLSFIDDQ 550 560 570 580 590 600 KTAFDVKDCK VFGGYVLHIG YLSYECDSLK VGDRVELTVD YTRRSPIMSN HTSTHMVNYA 610 620 630 640 650 660 LKNILGDGID QRGSFVDAQR FRFDFSFGRA ITKDELIKID QIVNDQIFKQ LDVHAKEVGL 670 680 690 700 710 720 ASAKKINGLR AVFGEVYPDP VRVVSVGVPV EQLIENPTNP EWANYSIEFC GGTHLSNTKQ 730 740 750 760 770 780 AELFTITSEE TLGAGVRRIV AVTGSEAASA IELNKELEVR FNNALKLSGS ELAKEIVSLL 790 800 810 820 830 840 DLLKVVTISA SVRMNLVETL KEVQALQRKQ VKEQETILAQ QAQTYLEKTS EELAKSQPKV 850 860 870 880 890 900 FVDLVNFNSN TPLITETIKK IQTKSPMTAI MLISPDEEKG KVTCIGIVPK DSEISKTLTA 910 920 930 940 NAWVVKVTEV LGGKGGGKVD VAQGVGSKLD KIDEAILVSR EFANAN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Detection of an intermediate stage of mitochondria genesis." Chihade J.W., Brown J.R., Schimmel P., Ribas de Pouplana L. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF188717 mRNA. Translation: AAF05592.1. AAFI02000023 Genomic DNA. Translation: EAL68084.1.
RefSeq	XP_642382.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3393158.
KEGG	ddi:DDB_0214894.
Organism-specific databases
dictyBase	DDB_G0277823. alaS.
Family and domain databases
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_DICDI

Accession

Primary (citable) accession number: Q54Y20
Secondary accession number(s): Q9U6B5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	May 24, 2005
Last modified:	November 25, 2008
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)