Reviewed, UniProtKB/Swiss-Prot Q55F21 (AATM_DICDI)

Names and origin

Protein names

Recommended name:
    Aspartate aminotransferase, mitochondrial
    EC=2.6.1.1
Alternative name(s):
    Transaminase A

Gene names

Name:	aatA
ORF Names:	DDB_G0268664

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion matrix. Cell membraneBy similarity.
Miscellaneous	In eukaryotes there are cytoplasmic and mitochondrial isozymes By similarity.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Mitochondrion
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartate transaminase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 29	29	Mitochondrion Potential
	Chain	30 – 426	397	Aspartate aminotransferase, mitochondrial		PRO_0000327597
Sites
	Binding site	273	1	Pyridoxal phosphate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q55F21-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 6E446EFFF83FD8CE	FASTA	426	47,285
	10 20 30 40 50 60 MIRSARLISN IKFGQKNIRQ FSTNTNWWAN VQKGPEDPIL GVSIAYNKDT SPSKINLGVG 70 80 90 100 110 120 AYRDENGKPY VLDCVKKADK KIYEANVDHE YAPIVGVAAF NQLAAQLALG EECKHIKEKR 130 140 150 160 170 180 IATVQSISGT GALRIAADFF ARFLKGKTAY VPNPTWGNHN VIFNDAGIPV KSYGYYNPAT 190 200 210 220 230 240 CGLNFEAMTK DIAAAPEGSI ILLHACAHNP TGVDPTAEQW KKISEICKER GHFVLFDFAY 250 260 270 280 290 300 QGFASGSPEK DAAAVRMFVE DGHNIALCQS FAKNFGLYGE RIGAFSILTE TSDQALNVES 310 320 330 340 350 360 QLKILIRPMY SNPPVYGARL VQAILKDKEL TNEWRSEVKG MADRIINMRE QLVKYLKKHG 370 380 390 400 410 420 STRDWSHITT QIGMFCFTGL TPEQVDRLAN EYHIYLTRNG RISIAGINST NVEYLAKAMA AVTKDN

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000004 Genomic DNA. Translation: EAL72921.1.
RefSeq	XP_646849.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3398182.
KEGG	ddi:DDB_0230092.
Organism-specific databases
dictyBase	DDB_G0268664. aatA.
Family and domain databases
InterPro	IPR004839. Aminotrans_I/II. IPR000796. Asp_trans. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11879. Asp_trans. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PRINTS	PR00799. TRANSAMINASE.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AATM_DICDI

Accession

Primary (citable) accession number: Q55F21

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 25, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)