Skip Header

 
Contribute Send feedback

Reviewed, UniProtKB/Swiss-Prot Q570C0 (TIR1_ARATH)

Last modified July 22, 2008. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Protein TRANSPORT INHIBITOR RESPONSE 1
Alternative name(s):
    F-box/LRR-repeat protein 1
Gene names
Name: TIR1
Synonyms: FBL1, WEI1
Ordered Locus Names: At3g62980
ORF Names: T20O10.80
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Hide | Top

Protein attributes

Sequence length594 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Auxin receptor that mediates Aux/IAA proteins proteasomal degradation and auxin-regulated transcription. The SCF(TIR1) E3 ubiquitin ligase complex is involved in auxin-mediated signaling pathway that regulate root and hypocotyl growth, lateral root formation, cell elongation, and gravitropism. Appears to allow pericycle cells to overcome G2 arrest prior to lateral root development. Plays a role in ethylene signaling in roots. Confers sensitivity to the virulent bacterial pathogen P.syringae.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with auxin. Part of a SCF E3 ubiquitin ligase complex SCF(TIR1) composed of SKP1, CUL1, RBX1 and TIR1. SCF(TIR1) interacts with the COP9 signalosome (CSN) complex. Interacts with Aux/IAA proteins (IAA3, IAA7, IAA12 and IAA17) in an auxin-dependent manner. The interaction with IAA3, a negative regulator of auxin responses, is promoted by auxin, but repressed by juglon (5-hydroxy-1,4-naphthoquinone).

Subcellular location

Nucleus.

Tissue specificity

Expressed in roots, stems, leaves and flowers. In adult plants, mostly expressed in floral stigma, anther filaments, abscission zones and vascular tissues.

Developmental stage

Abundant expression in developing embryos. In young seedlings, expressed in root apical meristem, and expanding cotyledons and hypocotyls. In older seedlings, still expressed in root apical meristems, but also in lateral root primordia, stipules, shoot apical meristem and vascular tissues.

Induction

Repressed by miR393a (microRNA) in response to flg-22 (flagellin-derived peptide 22).

Domain

The F-box is necessary for the interaction with SKP1.

Miscellaneous

Plant lacking TIR1 are deficient in a variety of auxin-regulated growth processes including lateral root formation, and hypocotyl and cell elongation.

Sequence similarities

Contains 1 F-box domain.

Contains 16 LRR (leucine-rich) repeats.

Hide | Top

Ontologies

Keywords

   Biological processAuxin signaling pathway
Cell cycle
Ethylene signaling pathway
Plant defense
Ubl conjugation pathway
   Cellular componentNucleus
   DomainLeucine-rich repeat
Repeat
   Molecular functionDevelopmental protein
   Technical term3D-structure
Complete proteome

Gene Ontology (GO)

   Molecular functionprotein binding Ref.7 Ref.9 Ref.10 Ref.12

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 594594Protein TRANSPORT INHIBITOR RESPONSE 1

Regions

Domain3 – 5048F-box
Repeat96 – 11318LRR 1
Repeat114 – 14633LRR 2
Repeat147 – 17428LRR 3
Repeat175 – 20127LRR 4
Repeat202 – 22827LRR 5
Repeat229 – 25527LRR 6
Repeat256 – 27924LRR 7
Repeat280 – 30425LRR 8
Repeat305 – 32824LRR 9
Repeat329 – 36234LRR 10
Repeat363 – 38725LRR 11
Repeat388 – 42235LRR 12
Repeat423 – 44927LRR 13
Repeat450 – 47122LRR 14
Repeat472 – 49322LRR 15
Repeat494 – 51724LRR 16

Experimental info

Mutagenesis101P → A: Abolishes SCF(TIR1) complex formation, altered auxin-mediated response and reduced affinity for auxin
Mutagenesis331V → A: No affinity for auxin
Mutagenesis351K → A: No affinity for auxin
Mutagenesis1471G → D in tir1-1; insensitive to auxin ubiquitously and to ethylene in roots only
Mutagenesis4411G → D in tir1-2; insensitive to auxin
Mutagenesis574 – 59421Missing in tir1-101/wei1; insensitive to auxin ubiquitously and to ethylene in roots only
Sequence conflict4901D → E in BAD94031. Ref.4
Sequence conflict5681D → G in BAD94031. Ref.4

Secondary structure

1 .................................................................................................. 594
Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q570C0-1 [UniParc].

Last modified June 21, 2005. Version 2.
Checksum: 9E19ED5DABF40D07

FASTA59466,799
        10         20         30         40         50         60 
MQKRIALSFP EEVLEHVFSF IQLDKDRNSV SLVCKSWYEI ERWCRRKVFI GNCYAVSPAT 

        70         80         90        100        110        120 
VIRRFPKVRS VELKGKPHFA DFNLVPDGWG GYVYPWIEAM SSSYTWLEEI RLKRMVVTDD 

       130        140        150        160        170        180 
CLELIAKSFK NFKVLVLSSC EGFSTDGLAA IAATCRNLKE LDLRESDVDD VSGHWLSHFP 

       190        200        210        220        230        240 
DTYTSLVSLN ISCLASEVSF SALERLVTRC PNLKSLKLNR AVPLEKLATL LQRAPQLEEL 

       250        260        270        280        290        300 
GTGGYTAEVR PDVYSGLSVA LSGCKELRCL SGFWDAVPAY LPAVYSVCSR LTTLNLSYAT 

       310        320        330        340        350        360 
VQSYDLVKLL CQCPKLQRLW VLDYIEDAGL EVLASTCKDL RELRVFPSEP FVMEPNVALT 

       370        380        390        400        410        420 
EQGLVSVSMG CPKLESVLYF CRQMTNAALI TIARNRPNMT RFRLCIIEPK APDYLTLEPL 

       430        440        450        460        470        480 
DIGFGAIVEH CKDLRRLSLS GLLTDKVFEY IGTYAKKMEM LSVAFAGDSD LGMHHVLSGC 

       490        500        510        520        530        540 
DSLRKLEIRD CPFGDKALLA NASKLETMRS LWMSSCSVSF GACKLLGQKM PKLNVEVIDE 

       550        560        570        580        590 
RGAPDSRPES CPVERVFIYR TVAGPRFDMP GFVWNMDQDS TMRFSRQIIT TNGL

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"The TIR1 protein of Arabidopsis functions in auxin response and is related to human SKP2 and yeast grr1p."
Ruegger M., Dewey E., Gray W.M., Hobbie L., Turner J., Estelle M.
Genes Dev. 12:198-207(1998) [PubMed: 9436980] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF GLY-147 AND GLY-441.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.