Reviewed, UniProtKB/Swiss-Prot Q06749 (ALGL_PSEAE)

Names and origin

Protein names

Recommended name:
    Alginate lyase
    EC=4.2.2.3
Alternative name(s):
    Poly(beta-D-mannuronate) lyase
    Poly(mana) alginate lyase

Gene names

Name:	algL
Ordered Locus Names:	PA3547

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Depolymerizes alginate by cleaving the beta-1,4 glycosidic bond. May enhance the production of alginate by controlling the length of the polymer chain during export.
Catalytic activity	Eliminative cleavage of polysaccharides containing beta-D-mannuronate residues to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at their ends.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the polysaccharide lyase 5 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alginic acid catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	poly(beta-D-mannuronate) lyase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 367	340	Alginate lyase		PRO_0000024918
Experimental info
	Sequence conflict	269	1	A → P in AAA71990. Ref.1
	Sequence conflict	337 – 341	5	KMLEA → NACSRP in AAA71990. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q06749-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: EAA3FE30032AB3BA	FASTA	367	40,829
	10 20 30 40 50 60 MKTSHLIRIA LPGALAAALL ASQVSQAADL VPPPGYYAAV GERKGSAGSC PAVPPPYTGS 70 80 90 100 110 120 LVFTSKYEGS DSARATLNVK AEKTFRSQIK DITDMERGAT KLVTQYMRSG RDGDLACALN 130 140 150 160 170 180 WMSAWARAGA LQSDDFNHTG KSMRKWALGS LSGAYMRLKF SSSRPLAAHA EQSREIEDWF 190 200 210 220 230 240 ARLGTQVVRD WSGLPLKKIN NHSYWAAWSV MSTAVVTNRR DLFDWAVSEF KVAANQVDEQ 250 260 270 280 290 300 GFLPNELKRR QRALAYHNYA LPPLAMIAAF AQVNGVDLRQ ENHGALQRLA ERVMKGVDDE 310 320 330 340 350 360 ETFEEKTGED QDMTDLKVDN KYAWLEPYCA LYRCEPKMLE AKKDREPFNS FRLGGEVTRV FSREGGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the algL gene of Pseudomonas aeruginosa and purification of its alginate lyase product." Boyd A., Ghosh M., May T.B., Shinabarger D., Keogh R., Chakrabarty A.M. Gene 131:1-8(1993) [PubMed: 8370530] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Characterization of the Pseudomonas aeruginosa alginate lyase gene (algL): cloning, sequencing, and expression in Escherichia coli." Schiller N.L., Monday S.R., Boyd C.M., Keen N.T., Ohman D.E. J. Bacteriol. 175:4780-4789(1993) [PubMed: 8335634] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FRD1.
[3]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L14597 Unassigned DNA. Translation: AAA71990.1. U27829 Genomic DNA. Translation: AAA91127.1. AE004091 Genomic DNA. Translation: AAG06935.1.
PIR	H83202. JN0777.
RefSeq	NP_252237.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	878552.
GenomeReviews	Gene locus PA3547 in contig AE004091_GR.
KEGG	pae:PA3547.
Organism-specific databases
PseudoCAP	PA3547.
CMR	Search...
Phylogenomic databases
HOGENOM	Q06749.
Enzyme and pathway databases
BioCyc	PAER208964:PA3547-MON.
Family and domain databases
HAMAP	MF_00557. [Tree]
InterPro	IPR008397. Alginate_lyase. [Graphical view]
Gene3D	G3DSA:1.50.10.110. Alginate_lyase. 1 hit.
Pfam	PF05426. Alginate_lyase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALGL_PSEAE

Accession

Primary (citable) accession number: Q06749
Secondary accession number(s): Q57292

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	December 15, 1998
Last modified:	November 25, 2008
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)