Reviewed, UniProtKB/Swiss-Prot Q57DY1 (RISB1_BRUAB)

Names and origin

Protein names

Recommended name:
    6,7-dimethyl-8-ribityllumazine synthase 1
      Short name=DMRL synthase 1
      Short name=Lumazine synthase 1
    EC=2.5.1.9
Alternative name(s):
    Riboflavin synthase 1 beta chain

Gene names

Name:	ribH1
Synonyms:	ribH, ribH-1
Ordered Locus Names:	BruAb1_0785

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes the condensation of 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione with L-3,4-dihydrohy-2-butanone-4-phosphate yielding 6,7-dimethyl-8-lumazine By similarity.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 6,7-dimethyl-8-(1-D-ribityl)lumazine and riboflavin from 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine: step 1/1.
Sequence similarities	Belongs to the DMRL synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Molecular function	Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	riboflavin synthase complex Inferred from electronic annotation. Source: InterPro
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 157

157

6,7-dimethyl-8-ribityllumazine synthase 1

PRO_0000134724

Secondary structure

1

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157

Helix Strand Turn

Details...

Beta strand

14 – 20

7

Helix

24 – 41

18

Beta strand

44 – 52

9

Helix

53 – 55

3

Helix

56 – 67

12

Turn

68 – 70

3

Beta strand

75 – 84

10

Beta strand

87 – 91

5

Helix

92 – 108

17

Beta strand

113 – 122

10

Helix

123 – 130

8

Turn

132 – 135

4

Helix

137 – 155

19

Sequences

	Sequence		Length	Mass (Da)
	Q57DY1-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 657DE105F885A875	FASTA	157	16,791
	10 20 30 40 50 60 MEFLMSKHEA DAPHLLIVEA RFYDDLADAL LDGAKAALDE AGATYDVVTV PGALEIPATI 70 80 90 100 110 120 SFALDGADNG GTEYDGFVAL GTVIRGETYH FDIVSNESCR ALTDLSVEES IAIGNGILTV 130 140 150 ENEEQAWVHA RREDKDKGGF AARAALTMIG LRKKFGA

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.

+

Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE017223 Genomic DNA. Translation: AAX74153.1.

RefSeq

YP_221514.1.

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2F59	X-ray	2.30	A/B/C/D/E	1-157	[»]
2I0F	X-ray	2.22	A/B/C/D/E	1-157	[»]

ModBase

Search...

Genome annotation databases

GeneID

3339926.

GenomeReviews

Gene locus BruAb1_0785 in contig AE017223_GR.

KEGG

bmb:BruAb1_0785.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

Q57DY1.

Enzyme and pathway databases

BioCyc

BABO262698:BRUAB1_0785-MON.

Family and domain databases

HAMAP

MF_00178.
[Tree]

InterPro

IPR002180. DMRL_synthase.
[Graphical view]

Gene3D

G3DSA:3.40.50.960. DMRL_synthase. 1 hit.

PANTHER

PTHR21058. DMRL_synthase. 1 hit.

Pfam

PF00885. DMRL_synthase. 1 hit.
[Graphical view]

ProDom

PD003664. DMRL_synthase. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00114. lumazine-synth. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RISB1_BRUAB

Accession

Primary (citable) accession number: Q57DY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	May 10, 2005
Last modified:	November 25, 2008
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)