Reviewed, UniProtKB/Swiss-Prot Q57DY5 (GLYA_BRUAB)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase
      Short name=Serine methylase
      Short name=SHMT
    EC=2.1.2.1

Gene names

Name:	glyA
Ordered Locus Names:	BruAb1_0781

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine.
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate pathway.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	Serine hydroxymethyltransferase		PRO_0000234953
Sites
	Binding site	242	1	Pyridoxal phosphate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57DY5-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: AF3745EA1E882CA6	FASTA	438	47,161
	10 20 30 40 50 60 MSQANAATKA YSDVFFNASL EDIDPEIFGA IRNELGRQRH EIELIASENI VSRAVLEAQG 70 80 90 100 110 120 SILTNKYAEG YPGKRYYGGC QYVDVVEELA IERAKKLFGA EFANVQPNSG SQMNQAVFLA 130 140 150 160 170 180 LLQPGDTFMG LDLNSGGHLT HGSPVNMSGK WFNVVSYGVR KDDHLLDMDE VARLARENKP 190 200 210 220 230 240 KLILAGGTAY SRIWDWKRFR EIADEVGAYL MVDMAHIAGL VAGGQHPSPV PHAHVCTTTT 250 260 270 280 290 300 HKSLRGPRGG MILTNDADIA KKINSAVFPG LQGGPLMHVI AGKAVAFAEA LKPEFKLYAK 310 320 330 340 350 360 NVVDNARALA EELKSHGLDI VSGGTDNHLM LVDLRPKNAT GKRAEAALGR ANITCNKNGI 370 380 390 400 410 420 PFDPEKPFVT SGVRLGTPAG TTRGFGVAEF KEIGSLIAEV LDGLKVANSD EGNAAVEQAV 430 KEKVIALTGR FPMYGYQG

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017223 Genomic DNA. Translation: AAX74149.1.
RefSeq	YP_221510.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3339541.
GenomeReviews	Gene locus BruAb1_0781 in contig AE017223_GR.
KEGG	bmb:BruAb1_0781.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q57DY5.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0781-MON.
Family and domain databases
HAMAP	MF_00051. [Tree]
InterPro	IPR001085. Gly_HO-MeTrfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA_BRUAB

Accession

Primary (citable) accession number: Q57DY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	May 10, 2005
Last modified:	November 25, 2008
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)