Reviewed, UniProtKB/Swiss-Prot Q57DZ8 (SYD_BRUAB)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	BruAb1_0768

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp).
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 595	595	Aspartyl-tRNA synthetase		PRO_0000235511

Sequences

	Sequence		Length	Mass (Da)
	Q57DZ8-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 6CAE4EDFF3F69E50	FASTA	595	67,255
	10 20 30 40 50 60 MHRYRSHTCA ALRKTDVGSN VRLSGWVHRV RDHGGILFID LRDHYGITQI VADPDSPAFK 70 80 90 100 110 120 VAETVRGEWV IRVDGEVKAR ADDAVNTNLP TGEVEIFATE IEVLSPAKEL PLPVFGEPDY 130 140 150 160 170 180 PEDIRLKYRF LDLRRETLHK NIMSRTKIIA AMRRRMTEIG FNEFSTPILT ASSPEGARDF 190 200 210 220 230 240 LVPSRIHPGK FYALPQAPQQ YKQLLMVAGF DRYFQIAPCF RDEDPRADRL PGEFYQLDLE 250 260 270 280 290 300 MSFVTQEEVW ETMEPVMRGI FEEFAEGKPV TKVFRRIAYD DAIRTYGSDK PDLRNPIEMQ 310 320 330 340 350 360 AVTDHFAGSG FKVFANMIAN DAKVEVWAIP AKTGGSRAFC DRMNSWAQSE GQPGLGYIFW 370 380 390 400 410 420 RKEGDKLEGA GPIAKNIGEE RTEAIRKQMG LEDGDACFFV AGLPSKFYKF AGDARTRAGE 430 440 450 460 470 480 ELNLVDRDRF ELAWIIDFPF YEWDEDNKKI DFAHNPFSLP QGGMDALENM DPLEIKAYQY 490 500 510 520 530 540 DLVCNGFEIA SGSIRNQLPE VMVKAFEKVG LSQQDVEERF GGLYRAFQYG APPHGGMAAG 550 560 570 580 590 IDRVIMLLVG AKNLREISLF PMNQQALDLL MGAPSEVSPA QLRDLHVRLA PVQKS

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017223 Genomic DNA. Translation: AAX74136.1.
RefSeq	YP_221497.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3339422.
GenomeReviews	Gene locus BruAb1_0768 in contig AE017223_GR.
KEGG	bmb:BruAb1_0768.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q57DZ8.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0768-MON.
Family and domain databases
HAMAP	MF_00044. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR006195. aa-tRNA-synth_II. IPR002312. Asp-tRNA-synth_IIb. IPR004524. Asp-tRNA-synth_IIb_bac/mito. IPR004115. GAD. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_BRUAB

Accession

Primary (citable) accession number: Q57DZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	May 10, 2005
Last modified:	November 25, 2008
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)