Reviewed, UniProtKB/Swiss-Prot Q57E58 (KSGA_BRUAB)

Names and origin

Protein names

Recommended name:
    Dimethyladenosine transferase
    EC=2.1.1.-
Alternative name(s):
    S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase
    16S rRNA dimethylase
    High level kasugamycin resistance protein ksgA
    Kasugamycin dimethyltransferase

Gene names

Name:	ksgA
Ordered Locus Names:	BruAb1_0701

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. Its inactivation leads to kasugamycin resistance By similarity.
Sequence similarities	Belongs to the rRNA adenine N(6)-methyltransferase family. KsgA subfamily.

Ontologies

Keywords
Biological process	Antibiotic resistance rRNA processing
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA modification Inferred from electronic annotation. Source: HAMAP response to antibiotic Inferred from electronic annotation. Source: HAMAP
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA (adenine-N6,N6-)-dimethyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	Dimethyladenosine transferase		PRO_0000271905
Sites
	Binding site	27	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	29	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	54	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	75	1	S-adenosyl-L-methionine By similarity
	Binding site	101	1	S-adenosyl-L-methionine By similarity
	Binding site	122	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57E58-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 63173E0F6332B6BB	FASTA	276	30,336
	10 20 30 40 50 60 MSIDSLPPLR EVIERHDLMP KKSLGQNFLF DLNLTSKIAR QAGDLRDQPV IEVGPGPGGL 70 80 90 100 110 120 TRALLAQGAY VTAIERDDRC LEALAEIAAH YPGRLRIIAG DALEQDFTAL FPEGPKPRIV 130 140 150 160 170 180 ANLPYNVGTQ LLLNWLLVEP WPPFYSSMTL MFQREVAERI VAKPDSDHYG RLGVLAGWRT 190 200 210 220 230 240 QAKIAFDVPP QAFTPPPKVM SSVVHIVPRE TPLPCRAEAL GQITQAAFGQ RRKMLRQSLK 250 260 270 SIGGAALLEK MGIDGTRRAE TLSVEEFVAL ANACLP

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017223 Genomic DNA. Translation: AAX74076.1.
RefSeq	YP_221437.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3339565.
GenomeReviews	Gene locus BruAb1_0701 in contig AE017223_GR.
KEGG	bmb:BruAb1_0701.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q57E58.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0701-MON.
Family and domain databases
HAMAP	MF_00607. [Tree]
InterPro	IPR011530. rRNA_adenine_dimethylase. IPR001737. RRNA_meth_trans. [Graphical view]
PANTHER	PTHR11727. RRNA_meth_trans. 1 hit.
Pfam	PF00398. RrnaAD. 1 hit. [Graphical view]
SMART	SM00650. rADc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00755. ksgA. 1 hit.
PROSITE	PS01131. RRNA_A_DIMETH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KSGA_BRUAB

Accession

Primary (citable) accession number: Q57E58

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	May 10, 2005
Last modified:	November 25, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)