Reviewed, UniProtKB/Swiss-Prot Q57E65 (SYC_BRUAB)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	BruAb1_0694

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Cysteinyl-tRNA synthetase		PRO_0000240893
Regions
	Motif	36 – 46	11	"HIGH" region
	Motif	302 – 306	5	"KMSKS" region
Sites
	Metal binding	34	1	Zinc By similarity
	Metal binding	230	1	Zinc By similarity
	Metal binding	269	1	Zinc By similarity
	Metal binding	273	1	Zinc By similarity
	Binding site	305	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57E65-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: 5B1DB5A1554DA639	FASTA	506	56,284
	10 20 30 40 50 60 MPDTTPQLRL YNTLTRTKEA FAPIDAKNVR MYVCGPTVYD FAHIGNARPV IVFDVLFRLL 70 80 90 100 110 120 RHVYGAQHVT YARNITDVDD KINARAARDY PDLPFNEAIR KVTESTNAQF QADVTALGNL 130 140 150 160 170 180 QPTVQPRATE HMDEMRAMID RLVQRGVAYV AQDHVLFSPS AMNARKGPRY GALARRSLDE 190 200 210 220 230 240 MLAGARVDVA SYKRDEMDFV LWKPSKKGEP GWPSPAGIET LGRPGWHIEC SAMSMAKLLE 250 260 270 280 290 300 PFGGGLKCDD PERNQFDIHG GGIDLVFPHH ENEIAQSCCA LGTERMANIW MHNGFLQVEG 310 320 330 340 350 360 QKMSKSLGNF ITIRDVLNDG LPQLGEWGDN TVRDHWAGLA ARLSMLQTHY REPINWTAQR 370 380 390 400 410 420 LAESADELHR WYGLLRDEGF GAPEKLSHAS AVAAALCDDL NSWAAITALR QAFKVRDVAA 430 440 450 460 470 480 LGEGMALMGL LDPYFVTASD VPIFARADVD ASAIAARIAE RLNFINAKNW AEADRIRDEL 490 500 LQEGVQLKDS KDPATGERIT TWDVVG

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017223 Genomic DNA. Translation: AAX74069.1.
RefSeq	YP_221430.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3339455.
GenomeReviews	Gene locus BruAb1_0694 in contig AE017223_GR.
KEGG	bmb:BruAb1_0694.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q57E65.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0694-MON.
Family and domain databases
HAMAP	MF_00041. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_BRUAB

Accession

Primary (citable) accession number: Q57E65

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	May 10, 2005
Last modified:	November 4, 2008
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)