Reviewed, UniProtKB/Swiss-Prot Q57ED2 (CCME_BRUAB)

Names and origin

Protein names

Recommended name:
    Cytochrome c-type biogenesis protein ccmE
Alternative name(s):
    Cytochrome c maturation protein E
    Heme chaperone ccmE

Gene names

Name:	ccmE
Synonyms:	cycJ
Ordered Locus Names:	BruAb1_0627

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by ccmC and transfers the heme to apo-cytochromes in a process facilitated by ccmF and ccmH By similarity.
Subcellular location	Cell inner membrane; Single-pass type II membrane protein; Periplasmic sidePotential.
Sequence similarities	Belongs to the ccmE/cycJ family.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal-anchor Transmembrane
Ligand	Heme Iron Metal-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cytochrome complex assembly Inferred from electronic annotation. Source: InterPro protein-heme linkage Inferred from electronic annotation. Source: HAMAP
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 165	165	Cytochrome c-type biogenesis protein ccmE		PRO_0000238798
Regions
	Topological domain	1 – 29	29	Cytoplasmic Potential
	Transmembrane	30 – 50	21	Signal-anchor for type II membrane protein Potential
	Topological domain	51 – 165	115	Periplasmic Potential
Sites
	Metal binding	147	1	Iron (heme axial ligand) By similarity
	Binding site	143	1	Heme (covalent; via pros nitrogen) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q57ED2-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: F67EB3C3641DCC6F	FASTA	165	17,977
	10 20 30 40 50 60 MSATAEQNAR NPKGKGGFAR TVSQRKRKRL FLIGGALAVL AVAVGLMLTA FNQDIRFFRT 70 80 90 100 110 120 PADLTEQDMT SGARFRLGGL VEEGSVSRTG SELRFTVTDT IKTVKVVFEG IPPDLFREGQ 130 140 150 160 GVVAEGRFGS DGLFRADNVL AKHDENYVPK DLADSLKKKG VWEGK

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017223 Genomic DNA. Translation: AAX74002.1.
RefSeq	YP_221363.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3339504.
GenomeReviews	Gene locus BruAb1_0627 in contig AE017223_GR.
KEGG	bmb:BruAb1_0627.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q57ED2.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0627-MON.
Family and domain databases
HAMAP	MF_01959. [Tree]
InterPro	IPR004329. CcmE. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF03100. CcmE. 1 hit. [Graphical view]
ProDom	PD006742. CcmE. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CCME_BRUAB

Accession

Primary (citable) accession number: Q57ED2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	May 10, 2005
Last modified:	November 25, 2008
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)