Reviewed, UniProtKB/Swiss-Prot Q57FS2 (ILVD_BRUAB)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BruAb1_0096

Organism

Brucella abortus [Complete proteome] [HAMAP]

Taxonomic identifier

235 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	611 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H(2)O.
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 611	611	Dihydroxy-acid dehydratase		PRO_0000225375
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q57FS2-1 [UniParc]. Last modified May 10, 2005. Version 1. Checksum: EB112A030C2D7831	FASTA	611	65,310
	10 20 30 40 50 60 MPPYRSRTTT HGRNMAGARG LWRATGMKDE DFGKPIIAVV NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AREIESAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSRELI ADSVEYMVNA HCADAMVCIS 130 140 150 160 170 180 NCDKITPGML MAALRLNIPV VFVSGGPMEA GKVVWEDSVK KLDLVDAMVA AADDHYTDEQ 190 200 210 220 230 240 VKAIERSACP TCGSCSGMFT ANSMNCLTEA LGLSLPGNGS TLATHADRKR LFVEAGHLIV 250 260 270 280 290 300 DLARRYYEQD DESVLPRSIA TFSAFENAMT LDIAMGGSTN TVLHLLAAAQ EAEIDFTMAD 310 320 330 340 350 360 IDRLSRRVPV LCKVAPAVSS VHMEDVHHAG GIMGILGQLD NAGLLTTSIP TVHSETLAKA 370 380 390 400 410 420 LDHWDVTRTN SEMVHKFYSA APGGVPTQVA FSQERRFDKV DTDREKGVIR SKEHAFSQDG 430 440 450 460 470 480 GLAVLYGNLA EDGCIVKTAG VDDSILKFSG PARIFESQDS AVLGILNGKI KPGDIVLIRY 490 500 510 520 530 540 EGPRGGPGMQ EMLYPTSYLK SKGLGKACAL ITDGRFSGGS SGLSIGHVSP EAAEGGTIGL 550 560 570 580 590 600 VREGDIIDID IPNRKIHLAV DDATLAERRA EQDAAGWKPA EERKRKISTA LKAYAAMATS 610 AARGAVRKLP D

References

[1]

"Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis." Halling S.M., Peterson-Burch B.D., Bricker B.J., Zuerner R.L., Qing Z., Li L.-L., Kapur V., Alt D.P., Olsen S.C. J. Bacteriol. 187:2715-2726(2005) [PubMed: 15805518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9-941 / Biovar 1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017223 Genomic DNA. Translation: AAX73512.1.
RefSeq	YP_220873.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3339002.
GenomeReviews	Gene locus BruAb1_0096 in contig AE017223_GR.
KEGG	bmb:BruAb1_0096.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q57FS2.
Enzyme and pathway databases
BioCyc	BABO262698:BRUAB1_0096-MON.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. ILVD_EDD_deHydtase. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BRUAB

Accession

Primary (citable) accession number: Q57FS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	May 10, 2005
Last modified:	November 4, 2008
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)