Reviewed, UniProtKB/Swiss-Prot Q59146 (E13B_ARTSW)

Names and origin

Protein names

Recommended name:
    Glucan endo-1,3-beta-glucosidase
    EC=3.2.1.39
Alternative name(s):
    (1->3)-beta-glucan endohydrolase
      Short name=(1->3)-beta-glucanase

Gene names

Name:

glcI

Organism

Arthrobacter sp. (strain YCWD3)

Taxonomic identifier

79671 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Arthrobacter

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Lysis of cellular walls containing beta-1,3-glucans. Implicated in the defense against fungal pathogens By similarity.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	PeriplasmBy similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the glycosyl hydrolase 64 family. Contains 1 ricin B-type lectin domain.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Periplasm
Domain	Signal
Ligand	Lectin
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 36	36	Tat-type signal Potential
	Chain	37 – 548	512	Glucan endo-1,3-beta-glucosidase		PRO_0000012234
Regions
	Domain	422 – 548	127	Ricin B-type lectin

Sequences

	Sequence		Length	Mass (Da)
	Q59146-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BD56354315750596	FASTA	548	58,164
	10 20 30 40 50 60 MPHDRKNSSR RAWAALCAAV LAVSGALVGV AAPASAVPAT IPLTITNDSG RGPIYLYVLG 70 80 90 100 110 120 ERDGVAGWAD AGGTFHPWPG GVGPVPVPAP DASIAGPGPG QSVTIRLPKL SGRVYYSYGQ 130 140 150 160 170 180 KMTFQIVLDG RLVQPAVQND SDPNRNILFN WTEYTLNDGG LWINSTQVDH WSAPYQVGVQ 190 200 210 220 230 240 RADGQVLSTG MLKPNGYEAF YTALESAGWG GLVQRAPDGS RLRALNPSHG IDVGKISSAS 250 260 270 280 290 300 IDSYVTEVWN SYRTRDMCVT PFSHEPGTQF RGRVDGDWFR FRNGSGQEVA AFKKPDASSV 310 320 330 340 350 360 YGCHKDLQAP NDHVVGPIAR TLCAALVRTT ALTNPNQPDA NSAGFYQDAR TNVYAKLAHQ 370 380 390 400 410 420 QMANGKAYAF AFDDVGAHES LVHDGNPQAA YIKLDPFTGT ATPIANGGST EQPGTPGGLP 430 440 450 460 470 480 AGTGALRIGS TLCLDVPWAD PTDTNQVQLA TCSGNAAQQW TRGTDGTVRA LGKCLDVARS 490 500 510 520 530 540 GTADGTAVWI YTCNGTGAQK WTYDSATKAL RNPQSGKCLD AQGGAPLRDG QKVQLWTCNQ TEAQRWTL

References

[1]	Watanabe T., Hasegawa H., Tanaka H., Doi A., Doi K. Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D23668 Genomic DNA. Translation: BAA04892.1.
3D structure databases
HSSP	HSSP built from PDB template 1KNM based on UniProtKB P26514.
ModBase	Search...
Family and domain databases
InterPro	IPR000772. Ricin_B_lectin. [Graphical view]
Pfam	PF00652. Ricin_B_lectin. 1 hit. [Graphical view]
SMART	SM00458. RICIN. 1 hit. [Graphical view]
PROSITE	PS50231. RICIN_B_LECTIN. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13B_ARTSW

Accession

Primary (citable) accession number: Q59146

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 4, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)