Reviewed, UniProtKB/Swiss-Prot Q59177 (CH60_BUCAP)

Names and origin

Protein names

Recommended name:
    60 kDa chaperonin
Alternative name(s):
    Protein Cpn60
    groEL protein

Gene names

Name:	groL
Synonyms:	groEL, mopA, symL
Ordered Locus Names:	BUsg_019

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions By similarity.
Subunit structure	Oligomer of 14 subunits composed of two stacked rings of 7 subunits By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the chaperonin (HSP60) family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein refolding Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP unfolded protein binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	60 kDa chaperonin		PRO_0000063306
Experimental info
	Sequence conflict	2	1	G → A in AAL30419. Ref.3
	Sequence conflict	181 – 187	7	TGLQNEL → QVFKNER in AAL30419. Ref.3
	Sequence conflict	266	1	S → P Ref.1 Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q59177-1 [UniParc]. Last modified November 25, 2002. Version 3. Checksum: 0007B98E93CA3491	FASTA	548	57,851
	10 20 30 40 50 60 MGAKDVKFGN EARIKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPSIT KDGVSVAREI 70 80 90 100 110 120 ELEDKFENMG AQMVKEVASK ANDAAGDGTT TATLLAQSIV NEGLKAVAAG MNPMDLKRGI 130 140 150 160 170 180 DKAVISAVEE LKNLSVPCSD SKAITQVGTI SANADEKVGA LIAEAMEKVG NDGVITVEEG 190 200 210 220 230 240 TGLQNELEVV KGMQFDRGYL SPYFINKPET GIVELENPYI LMADKKISNV REMLPILESV 250 260 270 280 290 300 AKSGKPLLII SEDLEGEALA TLVVNSMRGI VKVAAVKAPG FGDRRKAMLQ DISILTGGSV 310 320 330 340 350 360 ISEELAMDLE KSTLEDLGQA KRVVINKDTT TIIGGSGEKQ AIQSRIGQIR QEIQEATSDY 370 380 390 400 410 420 DKEKLNERLA KLSGGVAVLK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALV 430 440 450 460 470 480 RVAGKISNLR GHNEDQNVGI RVALRAMEAP LRQIVSNSGE EPSVVTNNVK DGKGNYGYNA 490 500 510 520 530 540 ATDEYGDMID FGILDPTKVT RSALQYAASV AGLMITTECM VTDLPREDKS SDVASSPAGG MGGMGGMM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of 60K, tdhF, groES and groEL genes of Buchnera aphidicola." Anwarul H.K., Moriya S., Baumann P., Yoshikawa H., Ogasawara N. Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of a 34.7-kb DNA segment from the genome of Buchnera aphidicola (endosymbiont of aphids) containing groEL, dnaA, the atp operon, gidA, and rho." Clark M.A., Baumann L., Baumann P. Curr. Microbiol. 36:158-163(1998) [PubMed: 9516544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Schizaphis graminum endosymbiont GroEL-related molecular chaperonin SymL (symL) gene." Wu Y., Lin L., Cui X. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D85628 Genomic DNA. Translation: BAA12847.1. Different initiation. AF008210 Genomic DNA. Translation: AAC38099.1. Different initiation. AF434719 Genomic DNA. Translation: AAL30419.1. AE013218 Genomic DNA. Translation: AAM67591.1.
RefSeq	NP_660380.1.
3D structure databases
HSSP	HSSP built from PDB template 1GR5 based on UniProtKB P06139.
SMR	Q59177. Positions 2-526.
ModBase	Search...
Genome annotation databases
GeneID	1005835.
GenomeReviews	Gene locus BUsg_019 in contig AE013218_GR.
KEGG	bas:BUsg019.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q59177.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG019-MON.
Family and domain databases
HAMAP	MF_00600. [Tree]
InterPro	IPR001844. Chaprnin_Cpn60. IPR002423. Cpn60/TCP-1. IPR012723. GroEL. [Graphical view]
PANTHER	PTHR11353. Cpn60/TCP-1. 1 hit.
Pfam	PF00118. Cpn60_TCP1. 1 hit. [Graphical view]
PRINTS	PR00298. CHAPERONIN60. PR00304. TCOMPLEXTCP1.
TIGRFAMs	TIGR02348. GroEL. 1 hit.
PROSITE	PS00296. CHAPERONINS_CPN60. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CH60_BUCAP

Accession

Primary (citable) accession number: Q59177
Secondary accession number(s): Q93A25

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 25, 2002
Last modified:	November 4, 2008
This is version 55 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)