Reviewed, UniProtKB/Swiss-Prot Q59478 (ALYA_KLEPN)

Names and origin

Protein names

Recommended name:
    Alginate lyase
    EC=4.2.2.3
Alternative name(s):
    Poly(beta-D-mannuronate) lyase
    Poly(mana) alginate lyase

Gene names

Name:

alyA

Organism

Klebsiella pneumoniae

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Degrades alginates that contain guluronic acid.
Catalytic activity	Eliminative cleavage of polysaccharides containing beta-D-mannuronate residues to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at their ends.
Subcellular location	Secreted.
Sequence similarities	Belongs to the polysaccharide lyase 7 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	poly(beta-D-mannuronate) lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20
	Chain	21 – 307	287	Alginate lyase		PRO_0000024925

Sequences

	Sequence		Length	Mass (Da)
	Q59478-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 76A1F9AAE082632E	FASTA	307	33,512
	10 20 30 40 50 60 MLKSGVMVAS LCLFSVPSRA AVPAPGDKFE LSGWSLSVPV DSDNDGKADQ IKEKTLAAGY 70 80 90 100 110 120 RNSDFFTLSD AGGMVFKAPI SGAKTSKNTT YTRSELREML RKGDTSIATQ GVSRNNWVLS 130 140 150 160 170 180 SAPLSEQKKA GGVDGTLEAT LSVDHVTTTG VNWQVGRVII GQIHANNDEP IRLYYRKLPH 190 200 210 220 230 240 HQKGSVYFAH EPRKGFGDEQ WYEMIGTLQP SHGNQTAAPT EPEAGIALGE TFSYRIDATG 250 260 270 280 290 300 NKLTVTLMRE GRPDVVKTVD MSKSGYSEAG QYLYFKAGVY NQNKTGKPDD YVQATFYRLK ATHGAQR

References

[1]

"Alginate lyase from Klebsiella pneumoniae, subsp. aerogenes: gene cloning, sequence analysis and high-level production in Escherichia coli." Baron A.J., Wong T.Y., Hicks S.J., Gacesa P., Willcock D., McPherson M.J. Gene 143:61-66(1994) [PubMed: 8200539] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 21-51. Strain: Subsp. aerogenes 25.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L19657 Genomic DNA. Translation: AAA25049.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR014895. Alginate_lyase_2. IPR013320. ConA_like_subgrp. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF08787. Alginate_lyase2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALYA_KLEPN

Accession

Primary (citable) accession number: Q59478

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 4, 2008
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)