Reviewed, UniProtKB/Swiss-Prot Q59662 (DHSB_PARDE)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase iron-sulfur subunit
    EC=1.3.99.1

Gene names

Name:

sdhB

Organism

Paracoccus denitrificans

Taxonomic identifier

266 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster By similarity. Binds 1 3Fe-4S cluster By similarity. Binds 1 4Fe-4S cluster By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, cytochrome b-556, and an hydrophobic anchor protein.
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW succinate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Succinate dehydrogenase iron-sulfur subunit		PRO_0000158689
Regions
	Domain	28 – 119	92	2Fe-2S ferredoxin-type
	Domain	160 – 190	31	4Fe-4S ferredoxin-type
Sites
	Metal binding	80	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	85	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	88	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	100	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	170	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	173	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	176	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	180	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	227	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	233	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	237	1	Iron-sulfur 2 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q59662-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F70C21BBA8205B4F	FASTA	259	29,551
	10 20 30 40 50 60 MVQLTLPKNS RMRVGKTWPK PEGATNVRRF NIYRWDPDTG ENPRIDTYFV DMDKCGPMVL 70 80 90 100 110 120 DALIKIKNEI DPTLTFRRSC REGICGSCAM NIDGGNHLAC IYGMDEIKGD VNIYPLPHMP 130 140 150 160 170 180 VVKDLVPDLT HFYAQHASVQ PYLITETPTP DKEWRQSIED RKKLDGLYEC VMCASCSTAC 190 200 210 220 230 240 PSYWWNGDRY LGPAALLHAY RWIIDSRDEA TGERLDSLED PFKLYRCHTI MNCTNTCPKG 250 LNPAKAIASI KHMMVDRIV

References

[1]

"Cloning, sequencing, and expression of the succinate-ubiquinone oxidoreductase (SdhCDAB) operon from Paracoccus denitrificans." Dickins M.A., Dhawan T., Gunsalus R.P., Schroeder I., Cecchini G. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13543 / NRRL B-3784.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U31902 Genomic DNA. Translation: AAA75178.1.
PIR	T46881.
3D structure databases
HSSP	HSSP built from PDB template 1NEK based on UniProtKB P07014.
ModBase	Search...
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR001450. 4Fe4S_Fe_S_bd. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR012285. Fum_reductase_C. IPR004489. Succ_DHase/fum_Rdtase_Fe-S. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSB_PARDE

Accession

Primary (citable) accession number: Q59662

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)