Reviewed, UniProtKB/Swiss-Prot Q5A4X0 (BRE1_CANAL)

Names and origin

Protein names

Recommended name:
    E3 ubiquitin-protein ligase BRE1
    EC=6.3.2.-

Gene names

Name:	BRE1
ORF Names:	CaO19.976, CaO19.8591

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	681 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H2B to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation By similarity.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	NucleusBy similarity.
Sequence similarities	Belongs to the BRE1 family. Contains 1 RING-type zinc finger.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Chromosomal protein Nucleus
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Chromatin regulator Ligase
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 681	681	E3 ubiquitin-protein ligase BRE1		PRO_0000055848
Regions
	Zinc finger	629 – 668	40	RING-type
	Coiled coil	50 – 77	28	Potential
	Coiled coil	136 – 190	55	Potential
	Coiled coil	224 – 446	223	Potential
	Coiled coil	477 – 628	152	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5A4X0-1 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 82E6002053F50C6C	FASTA	681	78,522
	10 20 30 40 50 60 MAVDNEKKRS TPDSLNSPHA KKSKPMQELS ESGPLTQADV VYFQKEAIWR QMIQYKQQMM 70 80 90 100 110 120 SMRSELIRLQ RESDASKHIV TVLTAWYEQI LALFDELNSE ETSDLLLAIT KNDDEQLDLI 130 140 150 160 170 180 RKKLSNLISS KVTFDSAKYE KVSGELALLS RQNEQMTEEK RSLGERIDEL ESKITELAKE 190 200 210 220 230 240 HERAQSKTLK RLDSQRHIKT EEDEQSTTKE ENPQPNGHHK ENDKTAVVDS EELDNLKCEL 250 260 270 280 290 300 DKLKTANQLL VQQVEQLTKE NQSLIQKSLN LENKLHNLEE SDLEDNTYYK KIVKNNQSLQ 310 320 330 340 350 360 EQIGKLTKLN SSNVSRLNEL ERQQNDLKSV IEGEIIEENE KLKQQLQESE NNLVRIRTTR 370 380 390 400 410 420 DELLSKNNIL TSQLQDQKTN ESLVELNNVL SKRVESLTEE RLEAIVGTPG TGKLEDLSQQ 430 440 450 460 470 480 ELIHKISQLN NEIKEVELAF KQTREITLKK LTSSIDQENL TKKLTIEKNK ADQKYFSAMR 490 500 510 520 530 540 VKDSLTNENK LLKAQIAKSQ DLIKNLNDLE KKYLNKIDLL SNQLVDFRII KENSLAENSK 550 560 570 580 590 600 LHDELKTLNI AQDALHQEVE RVNAKLESTL KEHTDLQESN KKRELELAKL QKQLQSTENI 610 620 630 640 650 660 LQKYKTNNTN SLLQEDEQQL EALRSIAKCS VCSKNWKDTA ITVCGHVFCS KCTQERLAAR 670 680 LRRCPSCNRG FSANDLLSIH L

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AACQ01000063 Genomic DNA. Translation: EAK97828.1. AACQ01000062 Genomic DNA. Translation: EAK97889.1.
RefSeq	XP_716814.1. XP_716874.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3641504. 3641568.
KEGG	cal:CaO19.8591. cal:CaO19.976.
Organism-specific databases
CGD	CAL0003779. BRE1.
Family and domain databases
InterPro	IPR013956. E3_ubiquit_lig_BRE1. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Gene3D	G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.
Pfam	PF08647. BRE1. 1 hit. PF00097. zf-C3HC4. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. [Graphical view]
PROSITE	PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BRE1_CANAL

Accession

Primary (citable) accession number: Q5A4X0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	April 26, 2005
Last modified:	November 25, 2008
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)