Reviewed, UniProtKB/Swiss-Prot Q5FH56 (SYA_EHRRG)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	ERGA_CDS_01420

Organism

Ehrlichia ruminantium (strain Gardel) [Complete proteome] [HAMAP]

Taxonomic identifier

302409 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Ehrlichia

Protein attributes

Sequence length	887 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm. HAMAP MF_00036
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 887	887	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000075109

Sequences

	Sequence		Length	Mass (Da)
	Q5FH56-1 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 2F68B292B0254480	FASTA	887	100,798
	10 20 30 40 50 60 MIKHNLVSDL RRLFIDFFVK NGHQFFPSSP LIIKDDPSLL FTNAGMVQFK QIFTSVDGRS 70 80 90 100 110 120 INTAVSSQKC LRVGGKHNDL ENVGHTNRHH TFFEMLGNFS FGSYFKEHAI ELAWNFVIKE 130 140 150 160 170 180 LALDRKRLYI TVYHDDQDAF NLWKKISSFS DDKIIKIKTN DNFWSMGNVG PCGPCSEIFY 190 200 210 220 230 240 DYGESVKGGL PGTPEEDGAR FTEIWNLVFM EYNRTKEGEL SVLPRKCIDT GMGLERIAAV 250 260 270 280 290 300 MQGVHDNYDI NLFKALIAMS KKESGNSSCE IAHRVIADHV RSAAFLIAEG LTPGNEGRDY 310 320 330 340 350 360 ILRRIIRRAA RYVYMLKYTD SLMYKIFPVL IDETSNAYMA DYYPELLKAK DMIISILKTE 370 380 390 400 410 420 EENFKDTLVR ALPLLEKELT YLSAGDVLSG DVIFRLYDTY GFPVDITLDI IKERGIRFDE 430 440 450 460 470 480 KGFYDNMEQQ KTRSRLSHLI KSTEQLNGKI WEDIRQNYNN TRFVGYDNFQ VQSKILSMVM 490 500 510 520 530 540 NNDRNVTVAN VGDKVSILMD VTPFYAEAGG QQADTGLLSV VRRDGKDLFG SSNIADVTNT 550 560 570 580 590 600 KNIFDGLYIH ECIIKSGSLI IGDIVSAEIN SHRRKDLCAN HSATHLLHYI LRMEIDNNIM 610 620 630 640 650 660 QKGSLVGNDK LRFDFSYNMA LTEKQIKLIE NRMCDLIRQN HPVETNICNL QDAMDNGAIA 670 680 690 700 710 720 LFTEKYDNHE VRVVNIGNSK ELCCGTHVKY TGEIGCFKII SESSIACGIR RIEAVTGQYA 730 740 750 760 770 780 IDYFRQQEKV LYQVAESVKS PVEDVLVQID KINRENQELK QKLWAAYFDI IDMQGVNIEK 790 800 810 820 830 840 IGNINFLHGT LSGVPIDVVR KFIMKRLVKD MIMLFSNVVN HNRIYVVGVG NSLHSKVKAA 850 860 870 880 DFVKIINCVV KSKGGGNAQL AQISTEYIAE VNVIQHIKDE LVSIFNA

References

[1]

"Comparative genomic analysis of three strains of Ehrlichia ruminantium reveals an active process of genome size plasticity." Frutos R., Viari A., Ferraz C., Morgat A., Eychenie S., Kandassamy Y., Chantal I., Bensaid A., Coissac E., Vachiery N., Demaille J., Martinez D. J. Bacteriol. 188:2533-2542(2006) [PubMed: 16547041] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR925677 Genomic DNA. Translation: CAI27594.1.
RefSeq	YP_196068.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3268694.
GenomeReviews	Gene locus ERGA_CDS_01420 in contig CR925677_GR.
KEGG	erg:ERGA_CDS_01420.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5FH56.
Enzyme and pathway databases
BioCyc	ERUM302409:ERGA_CDS_01420-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_EHRRG

Accession

Primary (citable) accession number: Q5FH56

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	March 1, 2005
Last modified:	July 22, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)