Reviewed, UniProtKB/Swiss-Prot Q5HVQ7 (SYA_CAMJR)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name(s)=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	CJE0613

Organism

Campylobacter jejuni (strain RM1221) [Complete proteome] [HAMAP]

Taxonomic identifier

195099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	842 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 842	842	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q5HVQ7-1 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: BA38E95A2B60A8B0	FASTA	842	94,920
	10 20 30 40 50 60 MDIRKAYLDF FASKGHEITP SSPLVPDDAT LLFTNAGMVP FKSIFTGEIP RPNPPRKTSC 70 80 90 100 110 120 QTCIRAGGKH NDLDNVGYTA RHHTFFEMLG NFSFGDYFKE QAIAYAWEFV TEVLKLPKDR 130 140 150 160 170 180 LYVTVHENDD EAFNLWQKHI QKERIYKFGD KDNFWQMGDT GPCGPCSEIF YDQGQEHFNS 190 200 210 220 230 240 SEDYMGGDGD RFLEIWNLVF MQYERSADGV LSPLPKPSID TGMGLERVTA IKEGKFSNFD 250 260 270 280 290 300 SSLFMPIINE ISKLCNKTYV YESGASFRVI ADHIRSSVFL LAQGVSFDKE GRGYVLRRIL 310 320 330 340 350 360 RRALRHGYLL GFKQAFMYKL VDIVCDLMGG HYTYLNEKKD FIKEQIRLEE ERFLSTIENG 370 380 390 400 410 420 IEIFNEELKN TKEIFSGEVA FKLYDTYGFP LDLTADMLRE KNLKVDEEKF ELFMNEQKAR 430 440 450 460 470 480 AKASWKGSGD KTASGDFKNL LEKFGENHFV GYEKAECESK ILALLDEDFK EVSTLKDAGW 490 500 510 520 530 540 VMLENTPFYA TSGGQSADSG FIAKREVLDT QKFFNLNLSF IKAGEELKVN DIVHARIDTE 550 560 570 580 590 600 KREQIARHHS ATHLLHHALR EILGSHVSQA GSLVESNKLR FDFTHHKALN KEELESIEKR 610 620 630 640 650 660 VNKMIINSSE AILENMPLEE AKKSGAIALF NEKYQGNVRV LTLGESKELC GGTHVKNTAQ 670 680 690 700 710 720 IGSFYIVKES GVSAGVRRIE AVVSKAALEF VKNQLEELSK VKDELKNNDI LSGIKKLKNE 730 740 750 760 770 780 ILSLKNELKN SSKTELDSKN IQGVEICVKR IDNGDIKAMI DDFKNKFAKA VILLIQVKDE 790 800 810 820 830 840 KITLAAGVKD VPLKAGALVK EAAQILGGNG GGRDDFATAG GKDLSKINEA LKQSLETIEK AL

References

[1]

"Major structural differences and novel potential virulence mechanisms from the genomes of multiple Campylobacter species." Fouts D.E., Mongodin E.F., Mandrell R.E., Miller W.G., Rasko D.A., Ravel J., Brinkac L.M., DeBoy R.T., Parker C.T., Daugherty S.C., Dodson R.J., Durkin A.S., Madupu R., Sullivan S.A., Shetty J.U., Ayodeji M.A., Shvartsbeyn A., Schatz M.C. , Badger J.H., Fraser C.M., Nelson K.E. PLoS Biol. 3:72-85(2005) [PubMed: 15660156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000025 Genomic DNA. Translation: AAW35874.1.
RefSeq	YP_178628.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3232064.
GenomeReviews	Gene locus CJE0613 in contig CP000025_GR.
KEGG	cjr:CJE0613.
NMPDR	fig|195099.3.peg.286.
TIGR	CJE0613.
Phylogenomic databases
HOGENOM	Q5HVQ7.
Enzyme and pathway databases
BioCyc	CJEJ195099:CJE_0613-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q5HVQ7. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_CAMJR

Accession

Primary (citable) accession number: Q5HVQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	February 15, 2005
Last modified:	July 22, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)