Reviewed, UniProtKB/Swiss-Prot Q5LHE6 (SYA_BACFN)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BF0689

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	872 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm. HAMAP MF_00036
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 872	872	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000075060

Sequences

	Sequence		Length	Mass (Da)
	Q5LHE6-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 9735927A3F33AF6B	FASTA	872	97,664
	10 20 30 40 50 60 MLTAKETRDS FKNFFESKGH QIVPSAPMVI KDDPTLMFTN AGMNQFKDII LGNHPAKYHR 70 80 90 100 110 120 VADSQKCLRV SGKHNDLEEV GHDTYHHTMF EMLGNWSFGD YFKKEAISWA WEYLVDVLKL 130 140 150 160 170 180 NPEHLYATVF EGSPEEGLER DNEAASYWEQ YLPKDHIING NKHDNFWEMG DTGPCGPCSE 190 200 210 220 230 240 IHIDLRPAEE RAKISGRDLV NHDHPQVIEI WNLVFMQYNR KADSTLEPLP AKVIDTGMGF 250 260 270 280 290 300 ERLCMALQGK TSNYDTDVFQ PLIKAIAQMA GTEYGKNEQN DIAMRVIADH IRTIAFSITD 310 320 330 340 350 360 GQLPSNAKAG YVIRRILRRA VRYGYTFLGQ KQAFMYKLLP VLIDSMGDAY PELIAQKELI 370 380 390 400 410 420 EKVIKEEEES FLRTLETGIR LLDKTMADTK ANGKTEISGK DAFTLYDTFG FPLDLTELIL 430 440 450 460 470 480 RENGMTVNVE EFDAEMQQQK QRARNAAAIE TGDWIILKEG TTEFVGYDYT EYETSILRYR 490 500 510 520 530 540 QVKQKNQTLY QIVLDYTPFY AESGGQVGDT GVLVNEFETI EVIDTKKENN LPIHITKKLP 550 560 570 580 590 600 EHPEAPMMAC VDTDKRAACA ANHSATHLLD EALREVLGEH VEQKGSLVTP DSLRFDFSHF 610 620 630 640 650 660 QKVTDEELRK VEHLVNAKIR ANVPLQEHRN IPIEEAKELG AIALFGEKYG DHVRVIQFGS 670 680 690 700 710 720 SIEFCGGTHV AATGNIGMVK IISESSVAAG VRRIEAYTGA RVEEMLDTIQ DTLSDLKALF 730 740 750 760 770 780 NNTPDLGVAI RKYIDENAGL KKQVEDFMKE KEAAVKERLL KNVQEINGIK VIKFCLPMPA 790 800 810 820 830 840 EVVKNIAFQL RGEITENLFF VAGTVDANKP MLTVMISDNL VAGGLKAGNL VKEAAKLIQG 850 860 870 GGGGQPHFAT AGGKNPDGLN AAVEKVLELA GI

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR626927 Genomic DNA. Translation: CAH06434.1.
RefSeq	YP_210392.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3289112.
GenomeReviews	Gene locus BF0689 in contig CR626927_GR.
KEGG	bfs:BF0689.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5LHE6.
Enzyme and pathway databases
BioCyc	BFRA272559:BF0689-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BACFN

Accession

Primary (citable) accession number: Q5LHE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	June 21, 2005
Last modified:	November 25, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)