Reviewed, UniProtKB/Swiss-Prot Q5NAT0 (GUN2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Endoglucanase 2
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta glucanase 2
    OsGLU5

Gene names

Name:	GLU5
Ordered Locus Names:	Os01g0220100, LOC_Os01g12070
ORF Names:	P0483F08.13, P0489G09.28

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	SecretedBy similarity.
Tissue specificity	Expressed in roots and flowers. Ref.3
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Potential
	Chain	35 – 640	606	Endoglucanase 2		PRO_0000249279
Sites
	Active site	428	1	By similarity
	Active site	480	1	By similarity
	Active site	489	1	By similarity
Amino acid modifications
	Glycosylation	528	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	616	1	G → S in AK103304. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q5NAT0-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: D68E16BB1B3A35CA	FASTA	640	68,408
	10 20 30 40 50 60 MARGGGAAGV SMAHHLGIAL VVLVFAAMAQ VARGGGGGHD YGMALSKSIL YFEAQRSGVL 70 80 90 100 110 120 PGSQRIAWRA NSGLADGKAN GVDLVGGYYD AGDNVKFGLP MAFTVTMMAW SVIEYGEEMA 130 140 150 160 170 180 AAGELGHAVE AIKWGTDYFA KAHPEPNVLY AEVGDGDSDH NCWQRPEDMT TSRQAYRLDP 190 200 210 220 230 240 QNPGSDLAGE TAAAMAAASL VFRSSNPGYA DQLLQHSKQL FDFADKYRGR YDNSITVARN 250 260 270 280 290 300 YYGSFSGYGD ELLWASAWLY QASDDRRYLD YLANNADALG GTGWSINQFG WDVKYPGVQI 310 320 330 340 350 360 LAAKFLLQGK AGEHAGVLQG YRRKADFFAC SCLGKDAADN VGRTPGGMLY HQRWNNIQFV 370 380 390 400 410 420 TSASFLLAVY SDHLAGGAVR CSGGGGAVAG AAELLAFAKS QVDYILGSNP RGTSYMVGYG 430 440 450 460 470 480 AVYPRQAHHR GSSIASIRAS PSFVSCREGY ASWYGRRGGN PNLLDGAVVG GPDEHDDFAD 490 500 510 520 530 540 ERNNYEQTEA ATYNNAPLMG ILARLAAGHG ARARGRLGQS LQHGIAANHT SLPHGANHQH 550 560 570 580 590 600 ASPVEIEQKA TASWEKDGRT YHRYAVTVSN RSPAGGKTVE ELHIGIGKLY GPVWGLEKAA 610 620 630 640 RYGYVLPSWT PSLPAGESAA FVYVHAAPPA DVWVTGYKLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[3]	"OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation." Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S., Chen S.-Y. Plant Mol. Biol. 60:137-151(2006) [PubMed: 16463105] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP002094 Genomic DNA. Translation: BAD81360.1. AP002745 Genomic DNA. Translation: BAD81426.1. AK103304 mRNA. No translation available.
RefSeq	NP_001042423.1.
UniGene	Os.32667
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4324643.
KEGG	osa:4324643.
Organism-specific databases
Gramene	Q5NAT0.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. False negative. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN2_ORYSJ

Accession

Primary (citable) accession number: Q5NAT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	February 1, 2005
Last modified:	November 4, 2008
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)