Reviewed, UniProtKB/Swiss-Prot Q5NAT8 (GUN1_ORYSJ)

Names and origin

Protein names

Recommended name:
    Endoglucanase 1
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta glucanase 1
    OsGLU7

Gene names

Name:	GLU7
Ordered Locus Names:	Os01g0219600, LOC_Os01g12030
ORF Names:	P0483F08.8, P0489G09.23

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	SecretedBy similarity.
Tissue specificity	Expressed in roots, leaf sheaths and flowers. Ref.2
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 640	616	Endoglucanase 1		PRO_0000249278
Sites
	Active site	433	1	By similarity
	Active site	485	1	By similarity
	Active site	494	1	By similarity
Amino acid modifications
	Glycosylation	528	1	N-linked (GlcNAc...) Potential
	Glycosylation	548	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5NAT8-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 3C9224D956FB441B	FASTA	640	67,904
	10 20 30 40 50 60 MARRGGAAAS SSMANLLGVA LVLAATAQTS ARGGGGGGRH DYRMALSKSI LYFEAQRSGV 70 80 90 100 110 120 LPGNQRIAWR ANSGLADGKA NGVDLVGGYY DAGDNVKFGF PMAFTVTMMA WSVLEYGKQM 130 140 150 160 170 180 AAAGELGHAM DAVRWGADYF VKAHPAPNVL YGEVGDGDSD HVCWQRPEDM TTSRQAYRLD 190 200 210 220 230 240 PQHPGSDLAG ETATALAAAS LVFRSSNPGY ANQLLQHSKQ LFDFADKYRG KYDDSMPVVK 250 260 270 280 290 300 KFYGSFSGYG DELLWASAWL YQATDNRRYL DYLANNGDAL GGTGWATNEF GWDVKYPGVQ 310 320 330 340 350 360 VLAAKFLLQG KAGPHAAVLR RYQRNADVFA CSCLGKGGGG GNVGRTPGGL MYHQGWNNLQ 370 380 390 400 410 420 FVTGASFLLA VYADHLAAAG RGQAVVRCQA GPAARASELV ALAKSQVDYI LGSNPRGISY 430 440 450 460 470 480 MVGYGARYPR RAHHRGASIV SIRANPSFVS CKDGYASWFG RAGSNPNLLD GAVVGGPDGR 490 500 510 520 530 540 DGFADERNNY QQTEVATYNN APLMGVLARL AGGGRGGLAE AAIKRPDNQT LLPPLAAAAS 550 560 570 580 590 600 PVEITQLNAT ASWKKDGRTY RRYAATVSNR SPAGGKTVEE LHIGIGKPHG PVWGLEKAAR 610 620 630 640 YGYVLPSSLA AGESAAFAYV VRGRAAPPPA DVWVIGYKLV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"OsGLU1, a putative membrane-bound endo-1,4-beta-D-glucanase from rice, affects plant internode elongation." Zhou H.-L., He S.-J., Cao Y.-R., Chen T., Du B.-X., Chu C.-C., Zhang J.-S., Chen S.-Y. Plant Mol. Biol. 60:137-151(2006) [PubMed: 16463105] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP002094 Genomic DNA. Translation: BAD81358.1. AP002745 Genomic DNA. Translation: BAD81424.1.
RefSeq	NP_001042421.1.
UniGene	Os.80657
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4324641.
KEGG	osa:4324641.
NMPDR	fig|39947.1.peg.5446.
Organism-specific databases
Gramene	Q5NAT8.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. False negative. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN1_ORYSJ

Accession

Primary (citable) accession number: Q5NAT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	February 1, 2005
Last modified:	November 4, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)