Reviewed, UniProtKB/Swiss-Prot Q5NAY4 (HISX_ORYSJ)

Names and origin

Protein names

Recommended name:
    Histidinol dehydrogenase, chloroplastic
      Short name=HDH
    EC=1.1.1.23

Gene names

Name:	HDH
Ordered Locus Names:	Os01g0232700, LOC_Os01g13190
ORF Names:	P0702F03.3

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine By similarity.
Catalytic activity	L-histidinol + 2 NAD(+) = L-histidine + 2 NADH.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.
Subcellular location	Plastid › chloroplastBy similarity.
Sequence similarities	Belongs to the histidinol dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Transit peptide	1 – ?		Chloroplast
	Chain	? – 473		Histidinol dehydrogenase, chloroplastic
Sites
	Active site	363	1	Proton acceptor By similarity
	Active site	364	1	Proton acceptor By similarity
	Metal binding	295	1	Zinc By similarity
	Metal binding	298	1	Zinc By similarity
	Metal binding	397	1	Zinc By similarity
	Metal binding	456	1	Zinc By similarity
	Binding site	162	1	NAD By similarity
	Binding site	224	1	NAD By similarity
	Binding site	247	1	NAD By similarity
	Binding site	273	1	Substrate By similarity
	Binding site	295	1	Substrate By similarity
	Binding site	298	1	Substrate By similarity
	Binding site	364	1	Substrate By similarity
	Binding site	397	1	Substrate By similarity
	Binding site	451	1	Substrate By similarity
	Binding site	456	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5NAY4-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: E46A7D59CEC1B412	FASTA	473	50,566
	10 20 30 40 50 60 MSLRPGRAHP LAASPLHTPL PARPRPQLRL STSTSCAAMK SYRLSELSDA EVGGLKARPR 70 80 90 100 110 120 IDFSSIFGTV NPIVEDVRMR GDAAVKDYTV KFDKVALDDV VVRVSDLPDV ELDPAVKEAF 130 140 150 160 170 180 DVAYDNIYAF HVSQKLPEKT VENMKGVRCK RITRCIGSVG LYVPGGTAVL PSTALMLAVP 190 200 210 220 230 240 AQIAGCKTVV LATPPSRDGS ICKEVLYCAK KAGVTHVLKA GGAQAISAMA WGTVSCPKVE 250 260 270 280 290 300 KIFGPGNQYV TAAKMILQNS EAMVSIDMPA GPSEVLVIAD KYANPVHVAA DLLSQAEHGP 310 320 330 340 350 360 DSQVVLVVAG DGVDLGAIEA EVSKQCSALP RGEFASKALG HSFTVFAKDM VEAISFSNMY 370 380 390 400 410 420 APEHLIINVK DAEQWEDLVE NAGSVFLGQW TPESVGDYAS GTNHVLPTYG YARMYSGVSL 430 440 450 460 470 NSFLKYITVQ SLSEEGLRSL GPHVAKMAEV EGLEAHRRAV TLRLQDIEAT VTV

References

[1]

"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

[2]

"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP002481 Genomic DNA. Translation: BAD81372.1. AK069972 mRNA. No translation available.
RefSeq	NP_001042506.1.
UniGene	Os.27540
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4324282.
KEGG	osa:4324282.
Organism-specific databases
Gramene	Q5NAY4.
Family and domain databases
InterPro	IPR001692. Histidinol_DHase. IPR012131. Hstdl_DHase_prok. [Graphical view]
PANTHER	PTHR21256:SF2. Hstdl_DH_prok. 1 hit.
Pfam	PF00815. Histidinol_dh. 1 hit. [Graphical view]
PRINTS	PR00083. HOLDHDRGNASE.
ProDom	PD002680. Histidinol_dh. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00069. hisD. 1 hit.
PROSITE	PS00611. HISOL_DEHYDROGENASE. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

HISX_ORYSJ

Accession

Primary (citable) accession number: Q5NAY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	February 1, 2005
Last modified:	July 22, 2008
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)