Reviewed, UniProtKB/Swiss-Prot Q5NAZ7 (GH33_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable indole-3-acetic acid-amido synthetase GH3.3
    EC=6.3.2.-
Alternative name(s):
    Auxin-responsive GH3-like protein 3
      Short name=OsGH3-3

Gene names

Name:	GH3.3
Ordered Locus Names:	Os01g0221100, LOC_Os01g12160
ORF Names:	P0483F08.33-1

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May catalyze the synthesis of indole-3-acetic acid (IAA)-amino acid conjugates, providing a mechanism for the plant to cope with the presence of excess auxin By similarity.
Tissue specificity	Expressed in green shoots and flowers.
Induction	At low level by auxin.
Sequence similarities	Belongs to the IAA-amido conjugating enzyme family.
Sequence caution	The sequence BAD81367.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Molecular function	Ligase
Gene Ontology (GO)
Biological process	response to auxin stimulus Inferred by curator. Source: Gramene response to light stimulus Inferred by curator. Source: Gramene
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 462	462	Probable indole-3-acetic acid-amido synthetase GH3.3
Experimental info
	Sequence conflict	362	1	P → H Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q5NAZ7-1 [UniParc]. Last modified November 22, 2005. Version 2. Checksum: 52FB68161F2947CC	FASTA	462	50,511
	10 20 30 40 50 60 MLEKKATRST RVDGVSGEAV IEEFERVTRD AANVQRETLR RILAENGGVE YLRGLGLAGA 70 80 90 100 110 120 TDPATFRARV PLATHADLEP YIDRIADGDA SPVLTAKPAT SISLSSGTTQ GKRKYLLFNE 130 140 150 160 170 180 ELVKSTMQIY RISYAFRNRE FPVENGKALQ FIYSSRETRT KGGLTATTAT TNVYRSEEFK 190 200 210 220 230 240 ATMRDIQSQC CSPDEVIFGP DFAQSLYCHL LAGLLAAGDV QIVSATFAHS VVLAFQTFER 250 260 270 280 290 300 AWEDLCADIR RGEVSPSRVT SPAVRRAMAA LLAAPNPGLA DEVARKCAAL SNWYGVIPAL 310 320 330 340 350 360 WPNARYVYGI MTGSMEHYVK KLRHYAGGLP LVAAEYGASE GWVGANVEPG TPPERATFTV 370 380 390 400 410 420 LPDIAYFEFI PLKPVAGDGG YAEAEPVGLT EVAAGELYEV VMTTFAGNTR SSSSCMTLVA 430 440 450 460 YYYLQSKKWM NICRFCISVH ETSRNLVTCT FAATGHIMFA QK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[3]	"The auxin-responsive GH3 gene family in rice (Oryza sativa)." Jain M., Kaur N., Tyagi A.K., Khurana J.P. Funct. Integr. Genomics 6:36-46(2006) [PubMed: 15856348] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION, NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP002094 Genomic DNA. Translation: BAD81367.1. Sequence problems. AK072125 mRNA. No translation available.
RefSeq	NP_001042430.1.
UniGene	Os.88968
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4324650.
KEGG	osa:4324650.
Organism-specific databases
Gramene	Q5NAZ7.
Family and domain databases
InterPro	IPR004993. GH3. [Graphical view]
Pfam	PF03321. GH3. 1 hit. [Graphical view]
ProDom	Q5NAZ7. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

GH33_ORYSJ

Accession

Primary (citable) accession number: Q5NAZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	July 22, 2008
This is version 19 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)