Reviewed, UniProtKB/Swiss-Prot Q5T0B8 (A3LT2_HUMAN)

Names and origin

Protein names

Recommended name:
    Alpha 1,3-galactosyltransferase 2
      Short name=A3galt2
    EC=2.4.1.87
Alternative name(s):
    Isoglobotriaosylceramide synthase
    iGb3 synthase
      Short name=iGb3S

Gene names

Name:

A3GALT2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transfer of galactose from UDP-galactose to an acceptor molecule (R). Synthesizes the galactose-alpha(1,3)-galactose group, which is the most common carbohydrate containing terminal alpha-galactose. Preferentially glycosylates lipids.
Catalytic activity	UDP-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatusPotential.
Domain	The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.
Sequence similarities	Belongs to the glycosyltransferase 6 family.

Ontologies

Keywords
Cellular component	Golgi apparatus
Domain	Signal
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 33	33	Potential
	Chain	34 – 340	307	Alpha 1,3-galactosyltransferase 2
Sites
	Metal binding	199	1	Manganese By similarity
	Metal binding	201	1	Manganese By similarity
Amino acid modifications
	Glycosylation	58	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5T0B8-1 [UniParc]. Last modified January 15, 2008. Version 2. Checksum: 129B673AC0DCE037	FASTA	340	38,754
	10 20 30 40 50 60 MALKEGLRAW KRIFWRQILL TLGLLGLFLY GLPKFRHLEA LIPMGVCPSA TMSQLRDNFT 70 80 90 100 110 120 GALRPWARPE VLTCTPWGAP IIWDGSFDPD VAKQEARQQN LTIGLTIFAV GRYLEKYLER 130 140 150 160 170 180 FLETAEQHFM AGQSVMYYVF TELPGAVPRV ALGPGRRLPV ERVARERRWQ DVSMARMRTL 190 200 210 220 230 240 HAALGGLPGR EAHFMFCMDV DQHFSGTFGP EALAESVAQL HSWHYHWPSW LLPFERDAHS 250 260 270 280 290 300 AAAMAWGQGD FYNHAAVFGG SVAALRGLTA HCAGGLDWDR ARGLEARWHD ESHLNKFFWL 310 320 330 340 HKPAKVLSPE FCWSPDIGPR AEIRRPRLLW APKGYRLLRN

References

[1]

"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL513327 Genomic DNA. Translation: CAI13954.1. Different initiation.
RefSeq	NP_001073907.1.
UniGene	Hs.653217
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000184389. Homo sapiens. [Contig view]
GeneID	127550.
KEGG	hsa:127550.
Organism-specific databases
HGNC	HGNC:30005. A3GALT2.
PharmGKB	PA142670461.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q5T0B8.
HOVERGEN	Q5T0B8.
Gene expression databases
ArrayExpress	Q5T0B8.
CleanEx	HS_A3GALT2.
Family and domain databases
InterPro	IPR005076. Glyco_trans_6. [Graphical view]
PANTHER	PTHR10462. Glyco_trans_6. 1 hit.
Pfam	PF03414. Glyco_transf_6. 1 hit. [Graphical view]
ProDom	Q5T0B8. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

A3LT2_HUMAN

Accession

Primary (citable) accession number: Q5T0B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	September 2, 2008
This is version 24 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)