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Reviewed, UniProtKB/Swiss-Prot Q5VST9 (OBSCN_HUMAN)

Last modified July 22, 2008. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (8) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Obscurin
    EC=2.7.11.1
Alternative name(s):
    Obscurin-myosin light chain kinase
      Short name=Obscurin-MLCK
    Obscurin-RhoGEF
Gene names
Name: OBSCN
Synonyms: KIAA1556, KIAA1639
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length7968 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Involved in myofibrillogenesis. Seems to be involved in assembly of myosin into sarcomeric A bands in striated muscle. Isoform 3 together with ANK1 isoform Mu17/Ank1.5 may provide a molecular link between the sarcoplasmic reticulum and myofibrils.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Subunit structure

Isoform 3 interacts with TTN/titin, ANK1 isoform Mu17/ank1.5, and with calmodulin in a Ca(2+)-independent manner. Associates with fast skeletal muscle myosin By similarity.

Subcellular location

Isoform 3: Cytoplasm › myofibril › sarcomere › M-band. Cytoplasm › myofibril › sarcomere › Z-disk. Note= In differentiating skeletal muscle cells, isoform 3 primarily localizes to the sarcomeric M-line and less frequently to the Z-disk. Isoform 3 colocalizes with ANK1 isoform Mu17/ank1.5 at the M-line in differentiated skeletal muscle cells.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.

Contains 1 DH (DBL-homology) domain.

Contains 3 fibronectin type-III domains.

Contains 55 Ig-like (immunoglobulin-like) domains.

Contains 1 IQ domain.

Contains 2 PH domains.

Contains 2 protein kinase domains.

Contains 1 SH3 domain.

Caution

Initially the name obscurin was used to describe isoform 3 which lacks the kinase domains.

Sequence caution

The sequence CAC85746.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAC85749.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAC85750.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAH71670.2 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAI19283.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAI19284.1 differs from that shown. Reason: Erroneous gene model prediction.

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Ontologies

Keywords

   Biological processDifferentiation
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainImmunoglobulin domain
Repeat
SH3 domain
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionDevelopmental protein
Kinase
Muscle protein
Serine/threonine-protein kinase
Transferase
   Technical term3D-structure

Gene Ontology (GO)

   Molecular functionprotein binding Ref.1 Ref.9

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

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Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Notes: Additional isoforms seem to exist.
Isoform 1 (identifier: Q5VST9-1)

Also known as: B; obscurin-MLCK giant kinase;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5VST9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     5753-5753: S → SS
Isoform 3 (identifier: Q5VST9-3)

Also known as: unc-89-like;

The sequence of this isoform differs from the canonical sequence as follows:
     6221-6620: DTTLERADQE...AQNITPAELQ → VTEQETKVPK...DRDGDEAAQP
     6621-7968: Missing.
Notes: In differentiating skeletal muscle cells, isoform 3 primarily localizes to the sarcomeric M-line and less frequently to the Z-disk. Isoform 3 colocalizes with ANK1 isoform Mu17/ank1.5 at the M-line in differentiated skeletal muscle cells. Lacks the kinase domain. Initially described as obscurin.
Isoform 4 (identifier: Q5VST9-4)

The sequence of this isoform differs from the canonical sequence as follows:
     3003-3010: RRVHIIED → EAAKCARA
     3011-7968: Missing.
Isoform 5 (identifier: Q5VST9-5)

The sequence of this isoform differs from the canonical sequence as follows:
     3888-3913: PQPVFREPLQSLQAEEGSTATLQCEL → LPARIHSRSEDQGGLRRGHSYTAV
     3914-7968: Missing.
Notes: No experimental confirmation available. Ref.6 (BAB13382) sequence differs from that shown due to frameshifts in positions 3891, 5061, 5093, 5268, 6352 and 6919.
Isoform 6 (identifier: Q5VST9-6)

The sequence of this isoform differs from the canonical sequence as follows:
     3886-3886: R → RALPARFTQD...EKTSATLTVK

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 79687968Obscurin

Regions

Domain10 – 10091Ig-like 1
Domain110 – 20293Ig-like 2
Domain236 – 32287Ig-like 3
Domain331 – 41484Ig-like 4
Domain420 – 50889Ig-like 5
Domain512 – 60796Fibronectin type-III 1
Domain619 – 69880Ig-like 6
Domain701 – 79090Ig-like 7
Domain798 – 88487Ig-like 8
Domain886 – 97792Ig-like 9
Domain978 – 106689Ig-like 10
Domain1070 – 116192Ig-like 11
Domain1162 – 125291Ig-like 12
Domain1254 – 134592Ig-like 13
Domain1346 – 143287Ig-like 14
Domain1438 – 152487Ig-like 15
Domain1530 – 162192Ig-like 16
Domain1622 – 171998Ig-like 17
Domain1731 – 180878Fibronectin type-III 2
Domain1809 – 189486Ig-like 18
Domain1896 – 198287Ig-like 19
Domain1987 – 207185Ig-like 20
Domain2077 – 216286Ig-like 21
Domain2165 – 224985Ig-like 22
Domain2289 – 238092Ig-like 23
Domain2468 – 255992Ig-like 24
Domain2564 – 264380Ig-like 25
Domain2646 – 273085Ig-like 26
Domain2736 – 282388Ig-like 27
Domain2826 – 290883Ig-like 28
Domain2920 – 299980Ig-like 29
Domain3003 – 309290Ig-like 30
Domain3095 – 318389Ig-like 31
Domain3184 – 326885Ig-like 32
Domain3273 – 335684Ig-like 33
Domain3359 – 344486Ig-like 34
Domain3449 – 353284Ig-like 35
Domain3537 – 362084Ig-like 36
Domain3625 – 370884Ig-like 37
Domain3713 – 379684Ig-like 38
Domain3801 – 388484Ig-like 39
Domain3890 – 397384Ig-like 40
Domain3978 – 406285Ig-like 41
Domain4068 – 416093Ig-like 42
Domain4171 – 423969Ig-like 43
Domain4248 – 433790Ig-like 44
Domain4340 – 442788Ig-like 45
Domain4430 – 451889Ig-like 46
Domain4523 – 461492Fibronectin type-III 3
Domain4624 – 471491Ig-like 47
Domain4872 – 490130IQ
Domain4898 – 498992Ig-like 48
Domain5126 – 521590Ig-like 49
Domain5260 – 534990Ig-like 50
Domain5371 – 546797Ig-like 51
Domain5601 – 566868SH3
Domain5693 – 5877185DH
Domain5895 – 6004110PH
Domain6014 – 609784Ig-like 52
Domain6108 – 620093Ig-like 53
Domain6357 – 644589Ig-like 54
Domain6468 – 6721254Protein kinase 1
Domain7463 – 755290Ig-like 55
Domain7557 – 764488Fibronectin type-III 4
Domain7672 – 7924253Protein kinase 2
Nucleotide binding6474 – 64829ATP By similarity
Nucleotide binding7678 – 76869ATP By similarity
Compositional bias192 – 1987Poly-Ala
Compositional bias710 – 7134Poly-Ala
Compositional bias931 – 9344Poly-Ser
Compositional bias1115 – 11184Poly-Ser
Compositional bias1207 – 12104Poly-Ser
Compositional bias1299 – 13024Poly-Ser
Compositional bias1391 – 13944Poly-Ser
Compositional bias1575 – 15784Poly-Ser
Compositional bias1667 – 16704Poly-Ser
Compositional bias4398 – 44014Poly-Leu
Compositional bias4846 – 48494Poly-Gln
Compositional bias6789 – 67968Poly-Ser
Compositional bias6969 – 7117149Pro-rich