Reviewed, UniProtKB/Swiss-Prot Q5VT25 (MRCKA_HUMAN)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase MRCK alpha
    EC=2.7.11.1
Alternative name(s):
    Myotonic dystrophy kinase-related CDC42-binding kinase alpha
      Short name=Myotonic dystrophy protein kinase-like alpha
      Short name=MRCK alpha
      Short name=CDC42-binding protein kinase alpha
    DMPK-like alpha

Gene names

Name:	CDC42BPA
Synonyms:	KIAA0451

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1732 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May act as a downstream effector of CDC42 in cytoskeletal reorganization. Contributes to the actomyosin contractility required for cell invasion, through the regulation of MYPT1 and thus MLC2 phosphorylation.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium.
Enzyme regulation	Maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation.
Subunit structure	Homodimer and homotetramer via the coiled coil regions. Interacts tightly with GTP-bound but not GDP-bound CDC42.
Subcellular location	CytoplasmBy similarity. Note= Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent By similarity.
Tissue specificity	Abundant in the heart, brain, skeletal muscle, kidney, and pancreas, with little or no expression in the lung and liver.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. DMPK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 CNH domain. Contains 1 CRIB domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Phorbol-ester binding Zinc-finger
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	actin cytoskeleton reorganization Ref.8 Inferred from direct assay. Source: UniProtKB protein amino acid phosphorylation Ref.5 Inferred from direct assay. Source: UniProtKB regulation of small GTPase mediated signal transduction Inferred from physical interaction. Source: UniProtKB
Cellular component	cell-cell junction Inferred from sequence or structural similarity. Source: UniProtKB leading edge Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Ref.5 Inferred from direct assay. Source: UniProtKB identical protein binding Ref.9 Inferred from physical interaction. Source: UniProtKB magnesium ion binding Ref.5 Inferred from direct assay. Source: UniProtKB protein serine/threonine kinase activity Ref.5 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-689171,EBI-689171
CDC42	P60953	3	EBI-689171,EBI-81752
RHOQ	P17081	1	EBI-689171,EBI-689202

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5VT25-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Notes: No experimental confirmation available.

Isoform 2 (identifier: Q5VT25-2)

The sequence of this isoform differs from the canonical sequence as follows:
     969-969: R → TDPVENTYVWNPSVKFHIQSRST
     973-981: CTPASKGRR → TSSEAEPVK

Notes: No experimental confirmation available.

Isoform 3 (identifier: Q5VT25-3)

The sequence of this isoform differs from the canonical sequence as follows:
     550-630: Missing.
     969-981: Missing.

Isoform 4 (identifier: Q5VT25-4)

The sequence of this isoform differs from the canonical sequence as follows:
     969-1009: Missing.

Notes: No experimental confirmation available.

Isoform 5 (identifier: Q5VT25-5)

The sequence of this isoform differs from the canonical sequence as follows:
     969-981: Missing.

Isoform 6 (identifier: Q5VT25-6)

The sequence of this isoform differs from the canonical sequence as follows:
     969-981: Missing.
     1597-1597: M → MPGFPYPSPHHHSGLISSPINFEHIYHMTVNSAEKFLSPDSINPEYSPSLRSVPGTPSFMTLR

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 1732	1732	Serine/threonine-protein kinase MRCK alpha
Regions
	Domain	77 – 343	267	Protein kinase
	Domain	344 – 414	71	AGC-kinase C-terminal
	Domain	1082 – 1201	120	PH
	Domain	1228 – 1499	272	CNH
	Domain	1571 – 1584	14	CRIB
	Nucleotide binding	83 – 91	9	ATP By similarity
	Zinc finger	1012 – 1062	51	Phorbol-ester/DAG-type
	Coiled coil	437 – 820	384	Potential
	Coiled coil	880 – 943	64	Potential
Sites
	Active site	201	1	Proton acceptor By similarity
	Binding site	106	1	ATP
Amino acid modifications
	Modified residue	222	1	Phosphoserine; by autocatalysis
	Modified residue	234	1	Phosphoserine; by autocatalysis
	Modified residue	240	1	Phosphothreonine; by autocatalysis
Natural variations
	Alternative sequence	550 – 630	81	Missing in isoform 3.
	Alternative sequence	969 – 1009	41	Missing in isoform 4.
	Alternative sequence	969 – 981	13	Missing in isoform 3, isoform 5 and isoform 6.
	Alternative sequence	969	1	R → TDPVENTYVWNPSVKFHIQS RST in isoform 2.
	Alternative sequence	973 – 981	9	CTPASKGRR → TSSEAEPVK in isoform 2.
	Alternative sequence	1597	1	M → MPGFPYPSPHHHSGLISSPI NFEHIYHMTVNSAEKFLSPD SINPEYSPSLRSVPGTPSFM TLR in isoform 6.
	Natural variant	50	1	E → K in a lung neuroendocrine carcinoma sample; somatic mutation.
	Natural variant	231	1	T → M
	Natural variant	537	1	I → T
	Natural variant	780	1	T → M: dbSNP rs56119119.
	Natural variant	790	1	Y → C: dbSNP rs34943764.
	Natural variant	1148	1	A → T
	Natural variant	1211	1	R → H
	Natural variant	1317	1	V → I
	Natural variant	1418	1	I → K
	Natural variant	1469	1	A → V: dbSNP rs55687355.
	Natural variant	1618	1	T → A
	Natural variant	1699	1	A → V: dbSNP rs2802269.
Experimental info
	Mutagenesis	106	1	K → A: Loss of kinase activity
	Mutagenesis	222	1	S → L: Increase in autophosphorylation but not kinase activity
	Mutagenesis	234	1	S → A: Loss of autophosphorylation and kinase activity
	Mutagenesis	240	1	T → A: Loss of autophosphorylation and kinase activity
	Mutagenesis	403	1	T → A: Loss of autophosphorylation and kinase activity
	Mutagenesis	1579	1	H → A: Loss of CDC42 binding; when associated with A-1582
	Mutagenesis	1582	1	H → A: Loss of CDC42 binding; when associated with A-1579
	Sequence conflict	25	1	C → Y in AAB37126. Ref.5
	Sequence conflict	809	1	D → N in CAI46252. Ref.3
	Sequence conflict	1521	1	L → V Ref.8
	Sequence conflict	1688	1	G → K in BAA32296. Ref.6
	Sequence conflict	1712	1	A → V in CAD57745 and CAD57746. Ref.1
	Sequence conflict	1712	1	A → V in BAD92205. Ref.2
	Sequence conflict	1712	1	A → V in CAI46252. Ref.3
	Sequence conflict	1712	1	A → V in BAA32296. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 48B10F81B5405A0A	FASTA	1,732	197,307
	10 20 30 40 50 60 MSGEVRLRQL EQFILDGPAQ TNGQCFSVET LLDILICLYD ECNNSPLRRE KNILEYLEWA 70 80 90 100 110 120 KPFTSKVKQM RLHREDFEIL KVIGRGAFGE VAVVKLKNAD KVFAMKILNK WEMLKRAETA 130 140 150 160 170 180 CFREERDVLV NGDNKWITTL HYAFQDDNNL YLVMDYYVGG DLLTLLSKFE DRLPEDMARF 190 200 210 220 230 240 YLAEMVIAID SVHQLHYVHR DIKPDNILMD MNGHIRLADF GSCLKLMEDG TVQSSVAVGT 250 260 270 280 290 300 PDYISPEILQ AMEDGKGRYG PECDWWSLGV CMYEMLYGET PFYAESLVET YGKIMNHKER 310 320 330 340 350 360 FQFPAQVTDV SENAKDLIRR LICSREHRLG QNGIEDFKKH PFFSGIDWDN IRNCEAPYIP 370 380 390 400 410 420 EVSSPTDTSN FDVDDDCLKN SETMPPPTHT AFSGHHLPFV GFTYTSSCVL SDRSCLRVTA 430 440 450 460 470 480 GPTSLDLDVN VQRTLDNNLA TEAYERRIKR LEQEKLELSR KLQESTQTVQ ALQYSTVDGP 490 500 510 520 530 540 LTASKDLEIK NLKEEIEKLR KQVTESSHLE QQLEEANAVR QELDDAFRQI KAYEKQIKTL 550 560 570 580 590 600 QQEREDLNKE LVQASERLKN QSKELKDAHC QRKLAMQEFM EINERLTELH TQKQKLARHV 610 620 630 640 650 660 RDKEEEVDLV MQKVESLRQE LRRTERAKKE LEVHTEALAA EASKDRKLRE QSEHYSKQLE 670 680 690 700 710 720 NELEGLKQKQ ISYSPGVCSI EHQQEITKLK TDLEKKSIFY EEELSKREGI HANEIKNLKK 730 740 750 760 770 780 ELHDSEGQQL ALNKEIMILK DKLEKTRRES QSEREEFESE FKQQYEREKV LLTEENKKLT 790 800 810 820 830 840 SELDKLTTLY ENLSIHNQQL EEEVKDLADK KESVAHWEAQ ITEIIQWVSD EKDARGYLQA 850 860 870 880 890 900 LASKMTEELE ALRNSSLGTR ATDMPWKMRR FAKLDMSARL ELQSALDAEI RAKQAIQEEL 910 920 930 940 950 960 NKVKASNIIT ECKLKDSEKK NLELLSEIEQ LIKDTEELRS EKGIEHQDSQ HSFLAFLNTP 970 980 990 1000 1010 1020 TDALDQFERS PSCTPASKGR RTVDSTPLSV HTPTLRKKGC PGSTGFPPKR KTHQFFVKSF 1030 1040 1050 1060 1070 1080 TTPTKCHQCT SLMVGLIRQG CSCEVCGFSC HITCVNKAPT TCPVPPEQTK GPLGIDPQKG 1090 1100 1110 1120 1130 1140 IGTAYEGHVR IPKPAGVKKG WQRALAIVCD FKLFLYDIAE GKASQPSVVI SQVIDMRDEE 1150 1160 1170 1180 1190 1200 FSVSSVLASD VIHASRKDIP CIFRVTASQL SASNNKCSIL MLADTENEKN KWVGVLSELH 1210 1220 1230 1240 1250 1260 KILKKNKFRD RSVYVPKEAY DSTLPLIKTT QAAAIIDHER IALGNEEGLF VVHVTKDEII 1270 1280 1290 1300 1310 1320 RVGDNKKIHQ IELIPNDQLV AVISGRNRHV RLFPMSALDG RETDFYKLSE TKGCQTVTSG 1330 1340 1350 1360 1370 1380 KVRHGALTCL CVAMKRQVLC YELFQSKTRH RKFKEIQVPY NVQWMAIFSE QLCVGFQSGF 1390 1400 1410 1420 1430 1440 LRYPLNGEGN PYSMLHSNDH TLSFIAHQPM DAICAVEISS KEYLLCFNSI GIYTDCQGRR 1450 1460 1470 1480 1490 1500 SRQQELMWPA NPSSCCYNAP YLSVYSENAV DIFDVNSMEW IQTLPLKKVR PLNNEGSLNL 1510 1520 1530 1540 1550 1560 LGLETIRLIY FKNKMAEGDE LVVPETSDNS RKQMVRNINN KRRYSFRVPE EERMQQRREM 1570 1580 1590 1600 1610 1620 LRDPEMRNKL ISNPTNFNHI AHMGPGDGIQ ILKDLPMNPR PQESRTVFSG SVSIPSITKS 1630 1640 1650 1660 1670 1680 RPEPGRSMSA SSGLSARSSA QNGSALKREF SGGSYSAKRQ PMPSPSEGSL SSGGMDQGSD 1690 1700 1710 1720 1730 APARDFDGED SDSPRHSTAS NSSNLSSPPS PASPRKTKSL SLESTDRGSW DP
	Isoform 2 [UniParc]. Checksum: F1CC5CC1ED23B388		1,754	199,811
	10 20 30 40 50 60 MSGEVRLRQL EQFILDGPAQ TNGQCFSVET LLDILICLYD ECNNSPLRRE KNILEYLEWA 70 80 90 100 110 120 KPFTSKVKQM RLHREDFEIL KVIGRGAFGE VAVVKLKNAD KVFAMKILNK WEMLKRAETA 130 140 150 160 170 180 CFREERDVLV NGDNKWITTL HYAFQDDNNL YLVMDYYVGG DLLTLLSKFE DRLPEDMARF 190 200 210 220 230 240 YLAEMVIAID SVHQLHYVHR DIKPDNILMD MNGHIRLADF GSCLKLMEDG TVQSSVAVGT 250 260 270 280 290 300 PDYISPEILQ AMEDGKGRYG PECDWWSLGV CMYEMLYGET PFYAESLVET YGKIMNHKER 310 320 330 340 350 360 FQFPAQVTDV SENAKDLIRR LICSREHRLG QNGIEDFKKH PFFSGIDWDN IRNCEAPYIP 370 380 390 400 410 420 EVSSPTDTSN FDVDDDCLKN SETMPPPTHT AFSGHHLPFV GFTYTSSCVL SDRSCLRVTA 430 440 450 460 470 480 GPTSLDLDVN VQRTLDNNLA TEAYERRIKR LEQEKLELSR KLQESTQTVQ ALQYSTVDGP 490 500 510 520 530 540 LTASKDLEIK NLKEEIEKLR KQVTESSHLE QQLEEANAVR QELDDAFRQI KAYEKQIKTL 550 560 570 580 590 600 QQEREDLNKE LVQASERLKN QSKELKDAHC QRKLAMQEFM EINERLTELH TQKQKLARHV 610 620 630 640 650 660 RDKEEEVDLV MQKVESLRQE LRRTERAKKE LEVHTEALAA EASKDRKLRE QSEHYSKQLE 670 680 690 700 710 720 NELEGLKQKQ ISYSPGVCSI EHQQEITKLK TDLEKKSIFY EEELSKREGI HANEIKNLKK 730 740 750 760 770 780 ELHDSEGQQL ALNKEIMILK DKLEKTRRES QSEREEFESE FKQQYEREKV LLTEENKKLT 790 800 810 820 830 840 SELDKLTTLY ENLSIHNQQL EEEVKDLADK KESVAHWEAQ ITEIIQWVSD EKDARGYLQA 850 860 870 880 890 900 LASKMTEELE ALRNSSLGTR ATDMPWKMRR FAKLDMSARL ELQSALDAEI RAKQAIQEEL 910 920 930 940 950 960 NKVKASNIIT ECKLKDSEKK NLELLSEIEQ LIKDTEELRS EKGIEHQDSQ HSFLAFLNTP 970 980 990 1000 1010 1020 TDALDQFETD PVENTYVWNP SVKFHIQSRS TSPSTSSEAE PVKTVDSTPL SVHTPTLRKK 1030 1040 1050 1060 1070 1080 GCPGSTGFPP KRKTHQFFVK SFTTPTKCHQ CTSLMVGLIR QGCSCEVCGF SCHITCVNKA 1090 1100 1110 1120 1130 1140 PTTCPVPPEQ TKGPLGIDPQ KGIGTAYEGH VRIPKPAGVK KGWQRALAIV CDFKLFLYDI 1150 1160 1170 1180 1190 1200 AEGKASQPSV VISQVIDMRD EEFSVSSVLA SDVIHASRKD IPCIFRVTAS QLSASNNKCS 1210 1220 1230 1240 1250 1260 ILMLADTENE KNKWVGVLSE LHKILKKNKF RDRSVYVPKE AYDSTLPLIK TTQAAAIIDH 1270 1280 1290 1300 1310 1320 ERIALGNEEG LFVVHVTKDE IIRVGDNKKI HQIELIPNDQ LVAVISGRNR HVRLFPMSAL 1330 1340 1350 1360 1370 1380 DGRETDFYKL SETKGCQTVT SGKVRHGALT CLCVAMKRQV LCYELFQSKT RHRKFKEIQV 1390 1400 1410 1420 1430 1440 PYNVQWMAIF SEQLCVGFQS GFLRYPLNGE GNPYSMLHSN DHTLSFIAHQ PMDAICAVEI 1450 1460 1470 1480 1490 1500 SSKEYLLCFN SIGIYTDCQG RRSRQQELMW PANPSSCCYN APYLSVYSEN AVDIFDVNSM 1510 1520 1530 1540 1550 1560 EWIQTLPLKK VRPLNNEGSL NLLGLETIRL IYFKNKMAEG DELVVPETSD NSRKQMVRNI 1570 1580 1590 1600 1610 1620 NNKRRYSFRV PEEERMQQRR EMLRDPEMRN KLISNPTNFN HIAHMGPGDG IQILKDLPMN 1630 1640 1650 1660 1670 1680 PRPQESRTVF SGSVSIPSIT KSRPEPGRSM SASSGLSARS SAQNGSALKR EFSGGSYSAK 1690 1700 1710 1720 1730 1740 RQPMPSPSEG SLSSGGMDQG SDAPARDFDG EDSDSPRHST ASNSSNLSSP PSPASPRKTK 1750 SLSLESTDRG SWDP
	Isoform 3 [UniParc]. Checksum: 7783E19090B345F7		1,638	186,113
	10 20 30 40 50 60 MSGEVRLRQL EQFILDGPAQ TNGQCFSVET LLDILICLYD ECNNSPLRRE KNILEYLEWA 70 80 90 100 110 120 KPFTSKVKQM RLHREDFEIL KVIGRGAFGE VAVVKLKNAD KVFAMKILNK WEMLKRAETA 130 140 150 160 170 180 CFREERDVLV NGDNKWITTL HYAFQDDNNL YLVMDYYVGG DLLTLLSKFE DRLPEDMARF 190 200 210 220 230 240 YLAEMVIAID SVHQLHYVHR DIKPDNILMD MNGHIRLADF GSCLKLMEDG TVQSSVAVGT 250 260 270 280 290 300 PDYISPEILQ AMEDGKGRYG PECDWWSLGV CMYEMLYGET PFYAESLVET YGKIMNHKER 310 320 330 340 350 360 FQFPAQVTDV SENAKDLIRR LICSREHRLG QNGIEDFKKH PFFSGIDWDN IRNCEAPYIP 370 380 390 400 410 420 EVSSPTDTSN FDVDDDCLKN SETMPPPTHT AFSGHHLPFV GFTYTSSCVL SDRSCLRVTA 430 440 450 460 470 480 GPTSLDLDVN VQRTLDNNLA TEAYERRIKR LEQEKLELSR KLQESTQTVQ ALQYSTVDGP 490 500 510 520 530 540 LTASKDLEIK NLKEEIEKLR KQVTESSHLE QQLEEANAVR QELDDAFRQI KAYEKQIKTL 550 560 570 580 590 600 QQEREDLNKL EVHTEALAAE ASKDRKLREQ SEHYSKQLEN ELEGLKQKQI SYSPGVCSIE 610 620 630 640 650 660 HQQEITKLKT DLEKKSIFYE EELSKREGIH ANEIKNLKKE LHDSEGQQLA LNKEIMILKD 670 680 690 700 710 720 KLEKTRRESQ SEREEFESEF KQQYEREKVL LTEENKKLTS ELDKLTTLYE NLSIHNQQLE 730 740 750 760 770 780 EEVKDLADKK ESVAHWEAQI TEIIQWVSDE KDARGYLQAL ASKMTEELEA LRNSSLGTRA 790 800 810 820 830 840 TDMPWKMRRF AKLDMSARLE LQSALDAEIR AKQAIQEELN KVKASNIITE CKLKDSEKKN 850 860 870 880 890 900 LELLSEIEQL IKDTEELRSE KGIEHQDSQH SFLAFLNTPT DALDQFETVD STPLSVHTPT 910 920 930 940 950 960 LRKKGCPGST GFPPKRKTHQ FFVKSFTTPT KCHQCTSLMV GLIRQGCSCE VCGFSCHITC 970 980 990 1000 1010 1020 VNKAPTTCPV PPEQTKGPLG IDPQKGIGTA YEGHVRIPKP AGVKKGWQRA LAIVCDFKLF 1030 1040 1050 1060 1070 1080 LYDIAEGKAS QPSVVISQVI DMRDEEFSVS SVLASDVIHA SRKDIPCIFR VTASQLSASN 1090 1100 1110 1120 1130 1140 NKCSILMLAD TENEKNKWVG VLSELHKILK KNKFRDRSVY VPKEAYDSTL PLIKTTQAAA 1150 1160 1170 1180 1190 1200 IIDHERIALG NEEGLFVVHV TKDEIIRVGD NKKIHQIELI PNDQLVAVIS GRNRHVRLFP 1210 1220 1230 1240 1250 1260 MSALDGRETD FYKLSETKGC QTVTSGKVRH GALTCLCVAM KRQVLCYELF QSKTRHRKFK 1270 1280 1290 1300 1310 1320 EIQVPYNVQW MAIFSEQLCV GFQSGFLRYP LNGEGNPYSM LHSNDHTLSF IAHQPMDAIC 1330 1340 1350 1360 1370 1380 AVEISSKEYL LCFNSIGIYT DCQGRRSRQQ ELMWPANPSS CCYNAPYLSV YSENAVDIFD 1390 1400 1410 1420 1430 1440 VNSMEWIQTL PLKKVRPLNN EGSLNLLGLE TIRLIYFKNK MAEGDELVVP ETSDNSRKQM 1450 1460 1470 1480 1490 1500 VRNINNKRRY SFRVPEEERM QQRREMLRDP EMRNKLISNP TNFNHIAHMG PGDGIQILKD 1510 1520 1530 1540 1550 1560 LPMNPRPQES RTVFSGSVSI PSITKSRPEP GRSMSASSGL SARSSAQNGS ALKREFSGGS 1570 1580 1590 1600 1610 1620 YSAKRQPMPS PSEGSLSSGG MDQGSDAPAR DFDGEDSDSP RHSTASNSSN LSSPPSPASP 1630 RKTKSLSLES TDRGSWDP
	Isoform 4 [UniParc]. Checksum: 28E904E222CFFEC5		1,691	193,031
	10 20 30 40 50 60 MSGEVRLRQL EQFILDGPAQ TNGQCFSVET LLDILICLYD ECNNSPLRRE KNILEYLEWA 70 80 90 100 110 120 KPFTSKVKQM RLHREDFEIL KVIGRGAFGE VAVVKLKNAD KVFAMKILNK WEMLKRAETA 130 140 150 160 170 180 CFREERDVLV NGDNKWITTL HYAFQDDNNL YLVMDYYVGG DLLTLLSKFE DRLPEDMARF 190 200 210 220 230 240 YLAEMVIAID SVHQLHYVHR DIKPDNILMD MNGHIRLADF GSCLKLMEDG TVQSSVAVGT 250 260 270 280 290 300 PDYISPEILQ AMEDGKGRYG PECDWWSLGV CMYEMLYGET PFYAESLVET YGKIMNHKER 310 320 330 340 350 360 FQFPAQVTDV SENAKDLIRR LICSREHRLG QNGIEDFKKH PFFSGIDWDN IRNCEAPYIP 370 380 390 400 410 420 EVSSPTDTSN FDVDDDCLKN SETMPPPTHT AFSGHHLPFV GFTYTSSCVL SDRSCLRVTA 430 440 450 460 470 480 GPTSLDLDVN VQRTLDNNLA TEAYERRIKR LEQEKLELSR KLQESTQTVQ ALQYSTVDGP 490 500 510 520 530 540 LTASKDLEIK NLKEEIEKLR KQVTESSHLE QQLEEANAVR QELDDAFRQI KAYEKQIKTL 550 560 570 580 590 600 QQEREDLNKE LVQASERLKN QSKELKDAHC QRKLAMQEFM EINERLTELH TQKQKLARHV 610 620 630 640 650 660 RDKEEEVDLV MQKVESLRQE LRRTERAKKE LEVHTEALAA EASKDRKLRE QSEHYSKQLE 670 680 690 700 710 720 NELEGLKQKQ ISYSPGVCSI EHQQEITKLK TDLEKKSIFY EEELSKREGI HANEIKNLKK 730 740 750 760 770 780 ELHDSEGQQL ALNKEIMILK DKLEKTRRES QSEREEFESE FKQQYEREKV LLTEENKKLT 790 800 810 820 830 840 SELDKLTTLY ENLSIHNQQL EEEVKDLADK KESVAHWEAQ ITEIIQWVSD EKDARGYLQA 850 860 870 880 890 900 LASKMTEELE ALRNSSLGTR ATDMPWKMRR FAKLDMSARL ELQSALDAEI RAKQAIQEEL 910 920 930 940 950 960 NKVKASNIIT ECKLKDSEKK NLELLSEIEQ LIKDTEELRS EKGIEHQDSQ HSFLAFLNTP 970 980 990 1000 1010 1020 TDALDQFERK THQFFVKSFT TPTKCHQCTS LMVGLIRQGC SCEVCGFSCH ITCVNKAPTT 1030 1040 1050 1060 1070 1080 CPVPPEQTKG PLGIDPQKGI GTAYEGHVRI PKPAGVKKGW QRALAIVCDF KLFLYDIAEG 1090 1100 1110 1120 1130 1140 KASQPSVVIS QVIDMRDEEF SVSSVLASDV IHASRKDIPC IFRVTASQLS ASNNKCSILM 1150 1160 1170 1180 1190 1200 LADTENEKNK WVGVLSELHK ILKKNKFRDR SVYVPKEAYD STLPLIKTTQ AAAIIDHERI 1210 1220 1230 1240 1250 1260 ALGNEEGLFV VHVTKDEIIR VGDNKKIHQI ELIPNDQLVA VISGRNRHVR LFPMSALDGR 1270 1280 1290 1300 1310 1320 ETDFYKLSET KGCQTVTSGK VRHGALTCLC VAMKRQVLCY ELFQSKTRHR KFKEIQVPYN 1330 1340 1350 1360 1370 1380 VQWMAIFSEQ LCVGFQSGFL RYPLNGEGNP YSMLHSNDHT LSFIAHQPMD AICAVEISSK 1390 1400 1410 1420 1430 1440 EYLLCFNSIG IYTDCQGRRS RQQELMWPAN PSSCCYNAPY LSVYSENAVD IFDVNSMEWI 1450 1460 1470 1480 1490 1500 QTLPLKKVRP LNNEGSLNLL GLETIRLIYF KNKMAEGDEL VVPETSDNSR KQMVRNINNK 1510 1520 1530 1540 1550 1560 RRYSFRVPEE ERMQQRREML RDPEMRNKLI SNPTNFNHIA HMGPGDGIQI LKDLPMNPRP 1570 1580 1590 1600 1610 1620 QESRTVFSGS VSIPSITKSR PEPGRSMSAS SGLSARSSAQ NGSALKREFS GGSYSAKRQP 1630 1640 1650 1660 1670 1680 MPSPSEGSLS SGGMDQGSDA PARDFDGEDS DSPRHSTASN SSNLSSPPSP ASPRKTKSLS 1690 LESTDRGSWD P
	Isoform 5 [UniParc]. Checksum: DBB15FA879AAC871		1,719	195,922
	10 20 30 40 50 60 MSGEVRLRQL EQFILDGPAQ TNGQCFSVET LLDILICLYD ECNNSPLRRE KNILEYLEWA 70 80 90 100 110 120 KPFTSKVKQM RLHREDFEIL KVIGRGAFGE VAVVKLKNAD KVFAMKILNK WEMLKRAETA 130 140 150 160 170 180 CFREERDVLV NGDNKWITTL HYAFQDDNNL YLVMDYYVGG DLLTLLSKFE DRLPEDMARF 190 200 210 220 230 240 YLAEMVIAID SVHQLHYVHR DIKPDNILMD MNGHIRLADF GSCLKLMEDG TVQSSVAVGT 250 260 270 280 290 300 PDYISPEILQ AMEDGKGRYG PECDWWSLGV CMYEMLYGET PFYAESLVET YGKIMNHKER 310 320 330 340 350 360 FQFPAQVTDV SENAKDLIRR LICSREHRLG QNGIEDFKKH PFFSGIDWDN IRNCEAPYIP 370 380 390 400 410 420 EVSSPTDTSN FDVDDDCLKN SETMPPPTHT AFSGHHLPFV GFTYTSSCVL SDRSCLRVTA 430 440 450 460 470 480 GPTSLDLDVN VQRTLDNNLA TEAYERRIKR LEQEKLELSR KLQESTQTVQ ALQYSTVDGP 490 500 510 520 530 540 LTASKDLEIK NLKEEIEKLR KQVTESSHLE QQLEEANAVR QELDDAFRQI KAYEKQIKTL 550 560 570 580 590 600 QQEREDLNKE LVQASERLKN QSKELKDAHC QRKLAMQEFM EINERLTELH TQKQKLARHV 610 620 630 640 650 660 RDKEEEVDLV MQKVESLRQE LRRTERAKKE LEVHTEALAA EASKDRKLRE QSEHYSKQLE 670 680 690 700 710 720 NELEGLKQKQ ISYSPGVCSI EHQQEITKLK TDLEKKSIFY EEELSKREGI HANEIKNLKK 730 740 750 760 770 780 ELHDSEGQQL ALNKEIMILK DKLEKTRRES QSEREEFESE FKQQYEREKV LLTEENKKLT 790 800 810 820 830 840 SELDKLTTLY ENLSIHNQQL EEEVKDLADK KESVAHWEAQ ITEIIQWVSD EKDARGYLQA 850 860 870 880 890 900 LASKMTEELE ALRNSSLGTR ATDMPWKMRR FAKLDMSARL ELQSALDAEI RAKQAIQEEL 910 920 930 940 950 960 NKVKASNIIT ECKLKDSEKK NLELLSEIEQ LIKDTEELRS EKGIEHQDSQ HSFLAFLNTP 970 980 990 1000 1010 1020 TDALDQFETV DSTPLSVHTP TLRKKGCPGS TGFPPKRKTH QFFVKSFTTP TKCHQCTSLM 1030 1040 1050 1060 1070 1080 VGLIRQGCSC EVCGFSCHIT CVNKAPTTCP VPPEQTKGPL GIDPQKGIGT AYEGHVRIPK 1090 1100 1110 1120 1130 1140 PAGVKKGWQR ALAIVCDFKL FLYDIAEGKA SQPSVVISQV IDMRDEEFSV SSVLASDVIH 1150 1160 1170 1180 1190 1200 ASRKDIPCIF RVTASQLSAS NNKCSILMLA DTENEKNKWV GVLSELHKIL KKNKFRDRSV 1210 1220 1230 1240 1250 1260 YVPKEAYDST LPLIKTTQAA AIIDHERIAL GNEEGLFVVH VTKDEIIRVG DNKKIHQIEL 1270 1280 1290 1300 1310 1320 IPNDQLVAVI SGRNRHVRLF PMSALDGRET DFYKLSETKG CQTVTSGKVR HGALTCLCVA 1330 1340 1350 1360 1370 1380 MKRQVLCYEL FQSKTRHRKF KEIQVPYNVQ WMAIFSEQLC VGFQSGFLRY PLNGEGNPYS 1390 1400 1410 1420 1430 1440 MLHSNDHTLS FIAHQPMDAI CAVEISSKEY LLCFNSIGIY TDCQGRRSRQ QELMWPANPS 1450 1460 1470 1480 1490 1500 SCCYNAPYLS VYSENAVDIF DVNSMEWIQT LPLKKVRPLN NEGSLNLLGL ETIRLIYFKN 1510 1520 1530 1540 1550 1560 KMAEGDELVV PETSDNSRKQ MVRNINNKRR YSFRVPEEER MQQRREMLRD PEMRNKLISN 1570 1580 1590 1600 1610 1620 PTNFNHIAHM GPGDGIQILK DLPMNPRPQE SRTVFSGSVS IPSITKSRPE PGRSMSASSG 1630 1640 1650 1660 1670 1680 LSARSSAQNG SALKREFSGG SYSAKRQPMP SPSEGSLSSG GMDQGSDAPA RDFDGEDSDS 1690 1700 1710 PRHSTASNSS NLSSPPSPAS PRKTKSLSLE STDRGSWDP