Reviewed, UniProtKB/Swiss-Prot Q5X2T6 (MDH_LEGPA)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase
    EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	lpp2301

Organism

Legionella pneumophila (strain Paris) [Complete proteome] [HAMAP]

Taxonomic identifier

297246 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD(+) = oxaloacetate + NADH.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 330	330	Malate dehydrogenase
Regions
	Nucleotide binding	12 – 18	7	NAD By similarity
	Nucleotide binding	130 – 132	3	NAD By similarity
Sites
	Active site	188	1	Proton acceptor By similarity
	Binding site	93	1	Substrate By similarity
	Binding site	99	1	Substrate By similarity
	Binding site	106	1	NAD By similarity
	Binding site	113	1	NAD By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	163	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5X2T6-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 3C36348E4E1BCAEA	FASTA	330	36,007
	10 20 30 40 50 60 MTNNRVRVAV TGAAGQIGYA LVFRIASGQM FGPNTEVELN LLELEPALPS LEGVAMELDD 70 80 90 100 110 120 CAFPLLKRIV CTADLNKAMD GVNWALLVGS VPRKQGMERS DLLQINGGIF TKQGQAINDY 130 140 150 160 170 180 ASDDVRVFVV GNPCNTNCLI AMNHAKDVPS DRFYAMTTLD ELRARTQLAK KAGVDITAVT 190 200 210 220 230 240 QMTIWGNHSA TQYPDFYNAK INGTSAAQVI NDETWLKETF VSTVQQRGAA VIKARGSSSA 250 260 270 280 290 300 ASAANAIITG VNHLVTDTPA GESFSMCRRS KGEYGVDEGL IFSFPCRREH GELKVVENLE 310 320 330 FNDFGRERFN TTLNELRSER DTVKSLGLLD

References

[1]

"Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity." Cazalet C., Rusniok C., Brueggemann H., Zidane N., Magnier A., Ma L., Tichit M., Jarraud S., Bouchier C., Vandenesch F., Kunst F., Etienne J., Glaser P., Buchrieser C. Nat. Genet. 36:1165-1173(2004) [PubMed: 15467720] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR628336 Genomic DNA. Translation: CAH13454.1.
RefSeq	YP_124612.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3119651.
GenomeReviews	Gene locus lpp2301 in contig CR628336_GR.
KEGG	lpp:lpp2301.
Organism-specific databases
LegioList	lpp2301.
CMR	Search...
Phylogenomic databases
HOGENOM	Q5X2T6.
Enzyme and pathway databases
BioCyc	LPNE297246:LPP2301-MON.
Family and domain databases
HAMAP	MF_01517. [Tree]
InterPro	IPR001557. L-lactate/malate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR001252. Malate_DHase_AS. IPR010945. Malate_DHase_SF1. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
ProDom	PD003052. Mal_dehydrog. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

MDH_LEGPA

Accession

Primary (citable) accession number: Q5X2T6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	November 23, 2004
Last modified:	July 22, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)