Reviewed, UniProtKB/Swiss-Prot Q60316 (FDHB_METJA)

Names and origin

Protein names

Recommended name:
    Formate dehydrogenase beta chain
    EC=1.2.1.2

Gene names

Name:	fdhB
Ordered Locus Names:	MJ0005

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of formate By similarity.
Catalytic activity	Formate + NAD(+) = CO(2) + NADH.
Cofactor	Binds 2 4Fe-4S clusters Probable.
Subunit structure	Dimer of alpha and beta chains By similarity.
Sequence similarities	Belongs to the frhB family. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Domain	Repeat
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	formate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 379	379	Formate dehydrogenase beta chain
Regions
	Domain	271 – 301	31	4Fe-4S ferredoxin-type 1
	Domain	321 – 351	31	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	280	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	283	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	286	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	290	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	330	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	333	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	336	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	340	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60316-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 9C257CCAD5547F5A	FASTA	379	43,014
	10 20 30 40 50 60 MKYVLIQATD NGILRRAECG GAVTALFKYL LDKKLVDGVL ALKRGEDVYD GIPTFITNSN 70 80 90 100 110 120 ELVETAGSLH CAPTNFGKLI AKYLADKKIA VPAKPCDAMA IRELAKLNQI NLDNVYMIGL 130 140 150 160 170 180 NCGGTISPIT AMKMIELFYE VNPLDVVKEE IDKGKFIIEL KNGEHKAVKI EELEEKGFGR 190 200 210 220 230 240 RKNCQRCEIM IPRMADLACG NWGAEKGWTF VEICSERGRK LVEDAEKDGY IKIKQPSEKA 250 260 270 280 290 300 IQVREKIESI MIKLAKKFQK KHLEEEYPSL EKWKKYWNRC IKCYGCRDNC PLCFCVECSL 310 320 330 340 350 360 EKDYIEEKGK IPPNPLIFQG IRLSHISQSC INCGQCEDAC PMDIPLAYIF HRMQLKIRDT 370 LGYIPGVDNS LPPLFNIER

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB97986.1.
PIR	E64300.
RefSeq	NP_246968.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1450844.
GenomeReviews	Gene locus MJ0005 in contig L77117_GR.
KEGG	mja:MJ0005.
NMPDR	fig|243232.1.peg.5.
TIGR	MJ0005.
Phylogenomic databases
HOGENOM	Q60316.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0005-MON.
Family and domain databases
InterPro	IPR001450. 4Fe4S_Fe_S_bd. IPR007516. Co_F420_Hydgase/DHase_bsu_N. IPR007525. FrhB_FdhB_C. [Graphical view]
Pfam	PF00037. Fer4. 1 hit. PF04432. FrhB_FdhB_C. 1 hit. PF04422. FrhB_FdhB_N. 1 hit. [Graphical view]
PRINTS	PR00353. 4FE4SFRDOXIN.
PROSITE	PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProDom	Q60316. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
LinkHub	Q60316.
ProtoNet	Search...

Entry information

Entry name

FDHB_METJA

Accession

Primary (citable) accession number: Q60316

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	July 22, 2008
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)