Reviewed, UniProtKB/Swiss-Prot Q60317 (AAT1_METJA)

Names and origin

Protein names

Recommended name:
    Probable aspartate aminotransferase 1
      Short name(s)=ASPAT
    EC=2.6.1.1
Alternative name(s):
    Transaminase A

Gene names

Ordered Locus Names:

MJ0001

Organism

Methanocaldococcus jannaschii (Methanococcus jannaschii) [Complete proteome] [HAMAP]

Taxonomic identifier

2190 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	aspartate transaminase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 375	375	Probable aspartate aminotransferase 1
Sites
	Binding site	223	1	Pyridoxal phosphate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60317-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 31349B11FC9B83A9	FASTA	375	42,396
	10 20 30 40 50 60 MISSRCKNIK PSAIREIFNL ATSDCINLGI GEPDFDTPKH IIEAAKRALD EGKTHYSPNN 70 80 90 100 110 120 GIPELREEIS NKLKDDYNLD VDKDNIIVTC GASEALMLSI MTLIDRGDEV LIPNPSFVSY 130 140 150 160 170 180 FSLTEFAEGK IKNIDLDENF NIDLEKVKES ITKKTKLIIF NSPSNPTGKV YDKETIKGLA 190 200 210 220 230 240 EIAEDYNLII VSDEVYDKII YDKKHYSPMQ FTDRCILING FSKTYAMTGW RIGYLAVSDE 250 260 270 280 290 300 LNKELDLINN MIKIHQYSFA CATTFAQYGA LAALRGSQKC VEDMVREFKM RRDLIYNGLK 310 320 330 340 350 360 DIFKVNKPDG AFYIFPDVSE YGDGVEVAKK LIENKVLCVP GVAFGENGAN YIRFSYATKY 370 EDIEKALGII KEIFE

References

[1]

"Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii." Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G., Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R., Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R., Kirkness E.F., Weinstock K.G. , Merrick J.M., Glodek A., Scott J.L., Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R., Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D., Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P., Fraser C.M., Smith H.O., Woese C.R., Venter J.C. Science 273:1058-1073(1996) [PubMed: 8688087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L77117 Genomic DNA. Translation: AAB97984.1.
PIR	A64300.
RefSeq	NP_246964.1.
3D structure databases
HSSP	HSSP built from PDB template 1DJU based on UniProtKB O59096.
ModBase	Search...
Genome annotation databases
GeneID	1450840.
GenomeReviews	Gene locus MJ0001 in contig L77117_GR.
KEGG	mja:MJ0001.
NMPDR	fig|243232.1.peg.1.
TIGR	MJ0001.
Phylogenomic databases
HOGENOM	Q60317.
Enzyme and pathway databases
BioCyc	MJAN243232:MJ_0001-MON.
Family and domain databases
InterPro	IPR001176. ACC_synthase. IPR004839. Aminotrans_I/II. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PRINTS	PR00753. ACCSYNTHASE.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProDom	Q60317. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

AAT1_METJA

Accession

Primary (citable) accession number: Q60317

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	July 22, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)