Reviewed, UniProtKB/Swiss-Prot Q60612 (AA1R_MOUSE)

Names and origin

Protein names

Recommended name:
    Adenosine receptor A1

Gene names

Name:

Adora1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Lipoprotein Palmitate
Gene Ontology (GO)
Biological process	regulation of excitatory postsynaptic membrane potential Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 326	326	Adenosine receptor A1
Regions
	Topological domain	1 – 10	10	Extracellular Potential
	Transmembrane	11 – 33	23	1 Potential
	Topological domain	34 – 46	13	Cytoplasmic Potential
	Transmembrane	47 – 69	23	2 Potential
	Topological domain	70 – 80	11	Extracellular Potential
	Transmembrane	81 – 102	22	3 Potential
	Topological domain	103 – 123	21	Cytoplasmic Potential
	Transmembrane	124 – 146	23	4 Potential
	Topological domain	147 – 176	30	Extracellular Potential
	Transmembrane	177 – 201	25	5 Potential
	Topological domain	202 – 235	34	Cytoplasmic Potential
	Transmembrane	236 – 259	24	6 Potential
	Topological domain	260 – 267	8	Extracellular Potential
	Transmembrane	268 – 292	25	7 Potential
	Topological domain	293 – 326	34	Cytoplasmic Potential
Amino acid modifications
	Lipidation	309	1	S-palmitoyl cysteine Potential
	Glycosylation	148	1	N-linked (GlcNAc...) Potential
	Glycosylation	159	1	N-linked (GlcNAc...) Potential
	Disulfide bond	80 ↔ 169		By similarity
Experimental info
	Sequence conflict	10	1	A → G Ref.1
	Sequence conflict	107	1	L → P in AAD31843. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q60612-1 [UniParc]. Last modified March 27, 2002. Version 3. Checksum: 6E5B72D94447963C	FASTA	326	36,652
	10 20 30 40 50 60 MPPYISAFQA AYIGIEVLIA LVSVPGNVLV IWAVKVNQAL RDATFCFIVS LAVADVAVGA 70 80 90 100 110 120 LVIPLAILIN IGPQTYFHTC LMVACPVLIL TQSSILALLA IAVDRYLRVK IPLRYKTVVT 130 140 150 160 170 180 QRRAAVAIAG CWILSLVVGL TPMFGWNNLS EVEQAWIANG SVGEPVIKCE FEKVISMEYM 190 200 210 220 230 240 VYFNFFVWVL PPLLLMVLIY LEVFYLIRKQ LNKKVSASSG DPQKYYGKEL KIAKSLALIL 250 260 270 280 290 300 FLFALSWLPL HILNCITLFC PTCQKPSILI YIAIFLTHGN SAMNPIVYAF RIHKFRVTFL 310 320 KIWNDHFRCQ PKPPIEEDIP EEKAED

References

[1]	"Cloning of two adenosine receptor subtypes from mouse bone marrow-derived mast cells." Marquardt D.L., Walker L.L., Heinemann S. J. Immunol. 152:4508-4515(1994) [PubMed: 8157966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Brain.
[2]	"Characterization of the murine A1 adenosine receptor promoter, potent regulation by GATA-4 and Nkx2.5." Rivkees S.A., Chen M., Kulkarni J., Browne J., Zhao Z. J. Biol. Chem. 274:14204-14209(1999) [PubMed: 10318839] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-107.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U05671 mRNA. Translation: AAA18999.1. Different initiation. AF133099 Genomic DNA. Translation: AAD31843.1.
UniGene	Mm.298908
3D structure databases
HSSP	HSSP built from PDB template 1MMH based on UniProtKB P29274.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSMUSG00000042429. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:99401. Adora1.
Phylogenomic databases
HOGENOM	Q60612.
HOVERGEN	Q60612.
Gene expression databases
ArrayExpress	Q60612.
CleanEx	MM_ADORA1.
GermOnline	ENSMUSG00000042429. Mus musculus.
Family and domain databases
InterPro	IPR001068. Adeno_A1_rcpt. IPR001634. Adenosn_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
PANTHER	PTHR19266:SF15. Adeno_A1_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00552. ADENOSINEA1R. PR00424. ADENOSINER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProDom	Q60612. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

AA1R_MOUSE

Accession

Primary (citable) accession number: Q60612
Secondary accession number(s): Q9WUF9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 27, 2002
Last modified:	July 22, 2008
This is version 64 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)