Reviewed, UniProtKB/Swiss-Prot Q60823 (AKT2_MOUSE)

Names and origin

Protein names

Recommended name:
    RAC-beta serine/threonine-protein kinase
    EC=2.7.11.1
Alternative name(s):
    RAC-PK-beta
    Protein kinase Akt-2
    Protein kinase B, beta
      Short name(s)=PKB beta

Gene names

Name:

Akt2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	General protein kinase capable of phosphorylating several known proteins.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Two specific sites, one in the kinase domain (Thr-309) and the other in the C-terminal regulatory region (Ser-474), need to be phosphorylated for its full activation By similarity.
Subunit structure	Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	regulation of JNK cascade Inferred from direct assay. Source: MGI
Cellular component	lamellipodium Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 481	481	RAC-beta serine/threonine-protein kinase
Regions
	Domain	5 – 108	104	PH
	Domain	152 – 409	258	Protein kinase
	Domain	410 – 481	72	AGC-kinase C-terminal
	Nucleotide binding	158 – 166	9	ATP By similarity
Sites
	Active site	275	1	Proton acceptor By similarity
	Binding site	181	1	ATP By similarity
Amino acid modifications
	Modified residue	309	1	Phosphothreonine; by PDPK1 By similarity
	Modified residue	474	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60823-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4AB4A9C4FB9CFA7D	FASTA	481	55,742
	10 20 30 40 50 60 MNEVSVIKEG WLHKRGEYIK TWRPRYFLLK SDGSFIGYKE RPEAPDQTLP PLNNFSVAEC 70 80 90 100 110 120 QLMKTERPRP NTFVIRCLQW TTVIERTFHV DSPDEREEWM RAIQMVANSL KQRGPGEDAM 130 140 150 160 170 180 DYKCGSPSDS STSEMMEVAV NKARAKVTMN DFDYLKLLGK GTFGKVILVR EKATGRYYAM 190 200 210 220 230 240 KILRKEVIIA KDEVAHTVTE SRVLQNTRHP FLTALKYAFQ THDRLCFVME YANGGELFFH 250 260 270 280 290 300 LSRERVFTED RARFYGAEIV SALEYLHSRD VVYRDIKLEN LMLDKDGHIK ITDFGLCKEG 310 320 330 340 350 360 ISDGATMKTF CGTPEYLAPE VLEDNDYGRA VDWWGLGVVM YEMMCGRLPF YNQDHERLFE 370 380 390 400 410 420 LILMEEIRFP RTLGPEAKSL LAGLLKKDPK QRLGGGPSDA KEVMEHRFFL SINWQDVVQK 430 440 450 460 470 480 KLLPPFKPQV TSEVDTRYFD DEFTAQSITI TPPDRYDSLD PLELDQRTHF PQFSYSASIR E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, chromosomal localization and expression analysis of the mouse Akt2 oncogene." Altomare D.A., Guo K., Cheng J.Q., Sonoda G., Walsh K., Testa J.R. Oncogene 11:1055-1060(1995) [PubMed: 7566964] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X CBA. Tissue: Thymus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Retina and Salivary gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U22445 mRNA. Translation: AAA83557.1. BC026151 mRNA. Translation: AAH26151.1. BC040377 mRNA. Translation: AAH40377.1.
RefSeq	NP_001103678.1. NP_031460.1. XP_001479789.1. XP_001479797.1. XP_001479802.1.
UniGene	Mm.177194
3D structure databases
HSSP	HSSP built from PDB template 1GZK based on UniProtKB P31751.
SMR	Q60823. Positions 1-111.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q60823.
PTM databases
PhosphoSite	Q60823.
Genome annotation databases
Ensembl	ENSMUSG00000004056. Mus musculus. [Contig view]
GeneID	100048123. 11652.
KEGG	mmu:100048123. mmu:11652.
Organism-specific databases
MGI	MGI:104874. Akt2.
Phylogenomic databases
HOGENOM	Q60823.
HOVERGEN	Q60823.
Gene expression databases
ArrayExpress	Q60823.
CleanEx	MM_AKT2.
GermOnline	ENSMUSG00000004056. Mus musculus.
Family and domain databases
InterPro	IPR015744. Akt. IPR001849. PH. IPR011993. PH_type. IPR000961. Pkinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
PANTHER	PTHR22985:SF69. Akt. 1 hit.
Pfam	PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00233. PH. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

AKT2_MOUSE

Accession

Primary (citable) accession number: Q60823

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	July 22, 2008
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)