Reviewed, UniProtKB/Swiss-Prot Q62KZ3 (SYA_BURMA)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BMA0895

Organism

Burkholderia mallei (Pseudomonas mallei) [Complete proteome] [HAMAP]

Taxonomic identifier

13373 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	874 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 874	874	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q62KZ3-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 3F520EF336ED4663	FASTA	874	94,685
	10 20 30 40 50 60 MKAAEIREKF LKFFESKGHT IVRSSSLVPG NDPTLLFTNS GMVQFKDVFL GAETRPYSRA 70 80 90 100 110 120 TTAQRSVRAG GKHNDLENVG YTARHHTFFE MLGNFSFGDY FKRDAIHYAW ELLTSVYKLP 130 140 150 160 170 180 ADKLWVTVYH DDDEAYDIWA KEVGVPAERI IRIGDNKGAR YASDNFWQMG DTGPCGPCSE 190 200 210 220 230 240 IFYDHGPDVW GGPPGSPEED GDRYIEIWNL VFMQFNRDAQ GNMTRLPKPC VDTGMGLERI 250 260 270 280 290 300 AAVLQHVHSN YEIDLFQQLI KASARETGVA DLANNSLKVI ADHIRACSFL IVDGVIPGNE 310 320 330 340 350 360 GRGYVLRRIV RRAIRHGYKL GRKAPFFHKL VADLVAEMGA AYPELKEAEP RVTDVLRQEE 370 380 390 400 410 420 ERFFETIEHG MSILEAALAE LDAAGGKTLD GELAFKLHDT YGFPLDLTAD VCRERGVTVD 430 440 450 460 470 480 EPAFDDAMAR QREQARAAGK FKATQGLEYT GAKTTFHGYE EIAFDDAKVV ALYVEGASVG 490 500 510 520 530 540 EVKAGESAVV VLDHTPFYAE SGGQVGDQGV LANAATRFAV GDTLKVQADV IGHHGELEQG 550 560 570 580 590 600 TLKVGDVVRA EIDAARRART ARNHSATHLM HKALRDVLGS HVQQKGSLVD ADKTRFDFAH 610 620 630 640 650 660 NAPLTDDEIR RVEAIVNEQV LANAPGIVRV MPYDDAVKGG AMALFGEKYG DEVRVLDLGF 670 680 690 700 710 720 SRELCGGTHV HRTGDIGLFK IVAEGGVAAG IRRVEAITGD NAVRYVQALD ARVNAAAAAL 730 740 750 760 770 780 KAQPSELLQR IGQVQDQVKS LEKELGALKS KLASSQGDEL AQQAVEVGGV HVLAATLDGA 790 800 810 820 830 840 DAKTLRETVD KLKDKLKSAA IVLAAVDGGK VSLIAGVTAD ASKKVKAGEL VNFVAQQVGG 850 860 870 KGGGRSDMAQ AGGTEPAKLP AALAGVKGWV EARL

References

[1]

"Structural flexibility in the Burkholderia mallei genome." Nierman W.C., DeShazer D., Kim H.S., Tettelin H., Nelson K.E., Feldblyum T.V., Ulrich R.L., Ronning C.M., Brinkac L.M., Daugherty S.C., Davidsen T.D., DeBoy R.T., Dimitrov G., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Khouri H.M. , Kolonay J.F., Madupu R., Mohammoud Y., Nelson W.C., Radune D., Romero C.M., Sarria S., Selengut J., Shamblin C., Sullivan S.A., White O., Yu Y., Zafar N., Zhou L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 101:14246-14251(2004) [PubMed: 15377793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23344.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000010 Genomic DNA. Translation: AAU49074.1.
RefSeq	YP_102626.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3090932.
GenomeReviews	Gene locus BMA0895 in contig CP000010_GR.
KEGG	bma:BMA0895.
TIGR	BMA0895.
Phylogenomic databases
HOGENOM	Q62KZ3.
Enzyme and pathway databases
BioCyc	BMAL243160:BMA_0895-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q62KZ3. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_BURMA

Accession

Primary (citable) accession number: Q62KZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	October 25, 2004
Last modified:	July 22, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)