Reviewed, UniProtKB/Swiss-Prot Q643R3 (PLCG_HUMAN)

Names and origin

Protein names

Recommended name:
    1-acyl-sn-glycerol-3-phosphate acyltransferase eta
    EC=2.3.1.51
Alternative name(s):
    1-AGP acyltransferase 7
      Short name=1-AGPAT 7
    Lysophosphatidic acid acyltransferase eta
      Short name=LPAAT-eta
    Acyltransferase-like 3

Gene names

Name:	AGPAT7
Synonyms:	AYTL3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone By similarity.
Catalytic activity	Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue specificity	Widely expressed. Expressed in uterus, thymus, pancreas, skeletal muscle, bladder, stomach, lung and testis.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.
Sequence caution	The sequence AAN33178.1 differs from that shown. Reason: Frameshift at position 198. The sequence AAP97722.1 differs from that shown. Reason: Frameshift at positions 188 and 198.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q643R3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q643R3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     415-447: LFAEEQAEGPNRLLYKDGFSTILHLLLGSPHPA → VMGGGGGGGCLVAMLTPLQEAYFGMLFPVASRY
     448-524: Missing.

Notes: No experimental confirmation available. Ref.4 (AAC19156) sequence differs from that shown due to a frameshift in position 327.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 524	524	1-acyl-sn-glycerol-3-phosphate acyltransferase eta
Regions
	Transmembrane	40 – 62	23	Potential
	Transmembrane	87 – 107	21	Potential
	Motif	129 – 134	6	HXXXXD motif
Amino acid modifications
	Glycosylation	152	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	415 – 447	33	LFAEE…SPHPA → VMGGGGGGGCLVAMLTPLQE AYFGMLFPVASRY in isoform 2.
	Alternative sequence	448 – 524	77	Missing in isoform 2.

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 3A2A12656E1241BC	FASTA	524	57,219
	10 20 30 40 50 60 MSQGSPGDWA PLDPTPGPPA SPNPFVHELH LSRLQRVKFC LLGALLAPIR VLLAFIVLFL 70 80 90 100 110 120 LWPFAWLQVA GLSEEQLQEP ITGWRKTVCH NGVLGLSRLL FFLLGFLRIR VRGQRASRLQ 130 140 150 160 170 180 APVLVAAPHS TFFDPIVLLP CDLPKVVSRA ENLSVPVIGA LLRFNQAILV SRHDPASRRR 190 200 210 220 230 240 VVEEVRRRAT SGGKWPQVLF FPEGTCSNKK ALLKFKPGAF IAGVPVQPVL IRYPNSLDTT 250 260 270 280 290 300 SWAWRGPGVL KVLWLTASQP CSIVDVEFLP VYHPSPEESR DPTLYANNVQ RVMAQALGIP 310 320 330 340 350 360 ATECEFVGSL PVIVVGRLKV ALEPQLWELG KVLRKAGLSA GYVDAGAEPG RSRMISQEEF 370 380 390 400 410 420 ARQLQLSDPQ TVAGAFGYFQ QDTKGLVDFR DVALALAALD GGRSLEELTR LAFELFAEEQ 430 440 450 460 470 480 AEGPNRLLYK DGFSTILHLL LGSPHPAATA LHAELCQAGS SQGLSLCQFQ NFSLHDPLYG 490 500 510 520 KLFSTYLRPP HTSRGTSQTP NASSPGNPTA LANGTVQAPK QKGD
	Isoform 2 [UniParc]. Checksum: FD4D1A528C0B8833		447	48,883
	10 20 30 40 50 60 MSQGSPGDWA PLDPTPGPPA SPNPFVHELH LSRLQRVKFC LLGALLAPIR VLLAFIVLFL 70 80 90 100 110 120 LWPFAWLQVA GLSEEQLQEP ITGWRKTVCH NGVLGLSRLL FFLLGFLRIR VRGQRASRLQ 130 140 150 160 170 180 APVLVAAPHS TFFDPIVLLP CDLPKVVSRA ENLSVPVIGA LLRFNQAILV SRHDPASRRR 190 200 210 220 230 240 VVEEVRRRAT SGGKWPQVLF FPEGTCSNKK ALLKFKPGAF IAGVPVQPVL IRYPNSLDTT 250 260 270 280 290 300 SWAWRGPGVL KVLWLTASQP CSIVDVEFLP VYHPSPEESR DPTLYANNVQ RVMAQALGIP 310 320 330 340 350 360 ATECEFVGSL PVIVVGRLKV ALEPQLWELG KVLRKAGLSA GYVDAGAEPG RSRMISQEEF 370 380 390 400 410 420 ARQLQLSDPQ TVAGAFGYFQ QDTKGLVDFR DVALALAALD GGRSLEELTR LAFEVMGGGG 430 440 GGGCLVAMLT PLQEAYFGML FPVASRY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization a novel human 1-acyl-sn-glycerol-3-phosphate acyltransferase gene AGPAT7." Ye G.-M., Chen C., Huang S., Han D.-D., Guo J.-H., Wan B., Yu L. DNA Seq. 16:386-390(2005) [PubMed: 16243729] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Testis.
[2]	Guo J.H., Yu L. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Ovary.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.
[4]	Yu W., Sarginson J., Gibbs R.A. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 268-524 (ISOFORM 2). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY734233 mRNA. Translation: AAU34184.1. AF529233 mRNA. Translation: AAP97722.1. Frameshift. AF542964 mRNA. Translation: AAN33178.1. Frameshift. BC024892 mRNA. Translation: AAH24892.2. BC092463 mRNA. Translation: AAH92463.1. AF007155 mRNA. Translation: AAC19156.1. Frameshift.
RefSeq	NP_705841.2.
UniGene	Hs.352614
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000176454. Homo sapiens. [Contig view]
GeneID	254531.
KEGG	hsa:254531.
Organism-specific databases
HGNC	HGNC:30059. AGPAT7.
PharmGKB	PA142672638.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	Q643R3.
HOVERGEN	Q643R3.
Gene expression databases
GermOnline	ENSG00000176454. Homo sapiens.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProDom	Q643R3. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

PLCG_HUMAN

Accession

Primary (citable) accession number: Q643R3
Secondary accession number(s): O43412 , Q7Z4P4, Q8IUL7, Q8TB38

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	October 25, 2004
Last modified:	September 2, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)