Reviewed, UniProtKB/Swiss-Prot Q64729 (TGFR1_MOUSE)

Names and origin

Protein names

Recommended name:
    TGF-beta receptor type-1
    EC=2.7.11.30
Alternative name(s):
    Transforming growth factor-beta receptor type I
      Short name=TGF-beta receptor type I
    TGF-beta type I receptor
    TbetaR-I
    TGFR-1
    ESK2

Gene names

Name:

Tgfbr1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for TGF-beta.
Catalytic activity	ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.
Cofactor	Magnesium or manganese By similarity.
Subunit structure	Interacts with CD109. The unphosphorylated protein interacts with FKBP1A and is stabilized the inactive conformation. Phosphorylation of the GS region abrogates FKBP1A binding. Interacts with SMAD2 when phosphorylated on several residues in the GS region By similarity.
Subcellular location	Membrane; Single-pass type I membrane proteinBy similarity.
Post-translational modification	Phosphorylated at basal levels in the absence of ligand binding. Activated by multiple phosphorylation, mainly in the GS region By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily. Contains 1 GS domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Kinase Receptor Serine/threonine-protein kinase Transferase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	anterior/posterior pattern formation Inferred from genetic interaction. Source: MGI artery morphogenesis Inferred from mutant phenotype. Source: MGI collagen fibril organization Inferred from mutant phenotype. Source: MGI embryonic cranial skeleton morphogenesis Inferred from mutant phenotype. Source: MGI germ cell migration Inferred from mutant phenotype. Source: MGI heart development Inferred from mutant phenotype. Source: MGI in utero embryonic development Inferred from mutant phenotype. Source: MGI induction of apoptosis Inferred from mutant phenotype. Source: MGI kidney development Inferred from genetic interaction. Source: MGI negative regulation of apoptosis Inferred from mutant phenotype. Source: MGI neuron fate commitment Inferred from mutant phenotype. Source: MGI palate development Inferred from mutant phenotype. Source: MGI parathyroid gland development Inferred from mutant phenotype. Source: MGI pharyngeal system development Inferred from mutant phenotype. Source: MGI positive regulation of filopodium formation Inferred from mutant phenotype. Source: MGI post-embryonic development Inferred from mutant phenotype. Source: MGI regulation of gene expression Inferred from mutant phenotype. Source: MGI thymus development Inferred from mutant phenotype. Source: MGI transforming growth factor beta receptor signaling pathway Inferred from direct assay. Source: MGI
Molecular function	protein binding Inferred from physical interaction. Source: MGI transforming growth factor beta receptor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q64729-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q64729-2)

The sequence of this isoform differs from the canonical sequence as follows:
     111-114: Missing.

Notes: May be due to a competing donnor splice site.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 503	481	TGF-beta receptor type-1
Regions
	Topological domain	23 – 125	103	Extracellular Potential
	Transmembrane	126 – 148	23	Potential
	Topological domain	149 – 503	355	Cytoplasmic Potential
	Domain	175 – 204	30	GS
	Domain	205 – 495	291	Protein kinase
	Nucleotide binding	211 – 219	9	ATP By similarity
Sites
	Active site	333	1	Proton acceptor By similarity
	Binding site	232	1	ATP By similarity
Amino acid modifications
	Modified residue	185	1	Phosphothreonine By similarity
	Modified residue	186	1	Phosphothreonine By similarity
	Modified residue	187	1	Phosphoserine By similarity
	Modified residue	189	1	Phosphoserine By similarity
	Modified residue	191	1	Phosphoserine By similarity
	Glycosylation	41	1	N-linked (GlcNAc...) Potential
	Disulfide bond	32 ↔ 50		By similarity
	Disulfide bond	34 ↔ 37		By similarity
	Disulfide bond	44 ↔ 67		By similarity
	Disulfide bond	82 ↔ 96		By similarity
	Disulfide bond	97 ↔ 102		By similarity
Natural variations
	Alternative sequence	111 – 114	4	Missing in isoform 2.

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BB8BB6D2261793AF	FASTA	503	56,179
	10 20 30 40 50 60 MEAAAAAPRR PQLLIVLVAA ATLLPGAKAL QCFCHLCTKD NFTCETDGLC FVSVTETTDK 70 80 90 100 110 120 VIHNSMCIAE IDLIPRDRPF VCAPSSKTGA VTTTYCCNQD HCNKIELPTT GPFSEKQSAG 130 140 150 160 170 180 LGPVELAAVI AGPVCFVCIA LMLMVYICHN RTVIHHRVPN EEDPSLDRPF ISEGTTLKDL 190 200 210 220 230 240 IYDMTTSGSG SGLPLLVQRT IARTIVLQES IGKGRFGEVW RGKWRGEEVA VKIFSSREER 250 260 270 280 290 300 SWFREAEIYQ TVMLRHENIL GFIAADNKDN GTWTQLWLVS DYHEHGSLFD YLNRYTVTVE 310 320 330 340 350 360 GMIKLALSTA SGLAHLHMEI VGTQGKPAIA HRDLKSKNIL VKKNGTCCIA DLGLAVRHDS 370 380 390 400 410 420 ATDTIDIAPN HRVGTKRYMA PEVLDDSINM KHFESFKRAD IYAMGLVFWE IARRCSIGGI 430 440 450 460 470 480 HEDYQLPYYD LVPSDPSVEE MRKVVCEQKL RPNIPNRWQS CEALRVMAKI MRECWYANGA 490 500 ARLTALRIKK TLSQLSQQEG IKM
	Isoform 2 [UniParc]. Checksum: 914DE6236B689C1C		499	55,790
	10 20 30 40 50 60 MEAAAAAPRR PQLLIVLVAA ATLLPGAKAL QCFCHLCTKD NFTCETDGLC FVSVTETTDK 70 80 90 100 110 120 VIHNSMCIAE IDLIPRDRPF VCAPSSKTGA VTTTYCCNQD HCNKIELPTT EKQSAGLGPV 130 140 150 160 170 180 ELAAVIAGPV CFVCIALMLM VYICHNRTVI HHRVPNEEDP SLDRPFISEG TTLKDLIYDM 190 200 210 220 230 240 TTSGSGSGLP LLVQRTIART IVLQESIGKG RFGEVWRGKW RGEEVAVKIF SSREERSWFR 250 260 270 280 290 300 EAEIYQTVML RHENILGFIA ADNKDNGTWT QLWLVSDYHE HGSLFDYLNR YTVTVEGMIK 310 320 330 340 350 360 LALSTASGLA HLHMEIVGTQ GKPAIAHRDL KSKNILVKKN GTCCIADLGL AVRHDSATDT 370 380 390 400 410 420 IDIAPNHRVG TKRYMAPEVL DDSINMKHFE SFKRADIYAM GLVFWEIARR CSIGGIHEDY 430 440 450 460 470 480 QLPYYDLVPS DPSVEEMRKV VCEQKLRPNI PNRWQSCEAL RVMAKIMREC WYANGAARLT 490 ALRIKKTLSQ LSQQEGIKM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a mouse counterpart for human TGF-beta type I receptor." Tomoda T., Kudoh T., Noma T., Nakazawa A., Muramatsu M.-A., Arai K. Biochem. Biophys. Res. Commun. 198:1054-1062(1994) [PubMed: 8117261] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain and Testis.
[2]	"A mouse TGF-beta type I receptor that requires type II receptor for ligand binding." Suzuki A., Shioda N., Maeda T., Tada M., Ueno N. Biochem. Biophys. Res. Commun. 198:1063-1069(1994) [PubMed: 8117262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D28526 mRNA. Translation: BAA05878.1. D25540 mRNA. Translation: BAA05023.1. BC063260 mRNA. Translation: AAH63260.1.
PIR	JC2061. JC2062.
RefSeq	NP_033396.1.
UniGene	Mm.197552
3D structure databases
HSSP	HSSP built from PDB template 1IAS based on UniProtKB P36897.
SMR	Q64729. Positions 201-503.
ModBase	Search...
PTM databases
PhosphoSite	Q64729.
Genome annotation databases
Ensembl	ENSMUSG00000007613. Mus musculus. [Contig view]
GeneID	21812.
KEGG	mmu:21812.
Organism-specific databases
MGI	MGI:98728. Tgfbr1.
Phylogenomic databases
HOGENOM	Q64729.
HOVERGEN	Q64729.
Gene expression databases
ArrayExpress	Q64729.
CleanEx	MM_TGFBR1.
GermOnline	ENSMUSG00000007613. Mus musculus.
Family and domain databases
InterPro	IPR000333. Activin_II_recpt. IPR000472. Activin_rcpt. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR003605. TGF_beta_rcpt_GS. [Graphical view]
Pfam	PF01064. Activin_recp. 1 hit. PF00069. Pkinase. 1 hit. PF08515. TGF_beta_GS. 1 hit. [Graphical view]
PRINTS	PR00653. ACTIVIN2R.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00467. GS. 1 hit. [Graphical view]
PROSITE	PS51256. GS. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
BLOCKS	Search...
Other Resources
SOURCE	Search...
ProtoNet	Search...

Entry information

Entry name

TGFR1_MOUSE

Accession

Primary (citable) accession number: Q64729

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	July 22, 2008
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)