Reviewed, UniProtKB/Swiss-Prot Q64YB4 (SYA_BACFR)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BF0761

Organism

Bacteroides fragilis [Complete proteome] [HAMAP]

Taxonomic identifier

817 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidetes (class) › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	872 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 872	872	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q64YB4-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 9670926E6E79FF85	FASTA	872	97,634
	10 20 30 40 50 60 MLTAKETRDS FKNFFESKGH QIVPSAPMVI KDDPTLMFTN AGMNQFKDII LGNHPAKYHR 70 80 90 100 110 120 VADSQKCLRV SGKHNDLEEV GHDTYHHTMF EMLGNWSFGD YFKKEAISWA WEYLVDVLKL 130 140 150 160 170 180 NPEHLYATVF EGSPEEGLER DNEAASYWEQ YLPKDHIING NKHDNFWEMG DTGPCGPCSE 190 200 210 220 230 240 IHIDLRPAEE RAKISGRDLV NHDHPQVIEI WNLVFMQYNR KADSTLEPLP AKVIDTGMGF 250 260 270 280 290 300 ERLCMALQGK TSNYDTDVFQ PLIKAIAQMA GTEYGKNEQN DIAMRVIADH IRTIAFSITD 310 320 330 340 350 360 GQLPSNAKAG YVIRRILRRA VRYGYTFLGQ KQAFMYKLLP VLIDSMGDAY PELIAQKELI 370 380 390 400 410 420 EKVIKEEEES FLRTLETGIR LLDKTMADTK ANGKTEISGK DAFTLYDTFG FPLDLTELIL 430 440 450 460 470 480 RENGMTVNVE EFDAEMQQQK QRARNAAAIE TGDWIILKEG TTEFVGYDYT EYETSILRYR 490 500 510 520 530 540 QVKQKNQTLY QIVLDYTPFY AESGGQVGDT GVLVNEFETI EVIDTKKENN LPIHITKKLP 550 560 570 580 590 600 EHPEAPMMAC VDTDKRAACA ANHSATHLLD EALREVLGEH VEQKGSLVTP DSLRFDFSHF 610 620 630 640 650 660 QKVTDEELRK VEHLVNAKIR ANVPLQEHRN IPIEEAKELG AIALFGEKYG DHVRVIQFGS 670 680 690 700 710 720 SIEFCGGTHV AATGNIGMVK IISESSVAAG VRRIEAYTGA RVEEMLDTIQ DTLSDLKALF 730 740 750 760 770 780 NNAPDLGVAI RKYIDENAGL KKQVEDFMKE KEAAVKERLL KNVQEINGIK VIKFCLPMPA 790 800 810 820 830 840 EVVKNIAFQL RGEITENLFF VAGTVDANKP MLTVMISDNL VAGGLKAGNL VKEAAKLIQG 850 860 870 GGGGQPHFAT AGGKNPDGLN AAVEKVLELA GI

References

[1]

"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation." Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y. Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed: 15466707] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: YCH46.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006841 Genomic DNA. Translation: BAD47512.1.
RefSeq	YP_098046.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3081614.
GenomeReviews	Gene locus BF0761 in contig AP006841_GR.
KEGG	bfr:BF0761.
NMPDR	fig|295405.3.peg.519.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q64YB4.
Enzyme and pathway databases
BioCyc	BFRA295405:BF0761-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q64YB4. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_BACFR

Accession

Primary (citable) accession number: Q64YB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	October 25, 2004
Last modified:	July 22, 2008
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)