Reviewed, UniProtKB/Swiss-Prot Q6AQ16 (SYA_DESPS)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name(s)=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	DP0828

Organism

Desulfotalea psychrophila [Complete proteome] [HAMAP]

Taxonomic identifier

84980 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfobacterales › Desulfobulbaceae › Desulfotalea

Protein attributes

Sequence length	882 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view
Molecule processing
	Chain	1 – 882	882	Alanyl-tRNA synthetase

Sequences

	Sequence		Length	Mass (Da)
	Q6AQ16-1 [UniParc]. Last modified September 13, 2004. Version 1. Checksum: A9706DFCDD323FE3	FASTA	882	94,583
	10 20 30 40 50 60 MKGNEIRSRF LEYFKGNGHT VAESSSLVPK DDPTLLFTNA GMVQFKRVFM GDDKRGYVRA 70 80 90 100 110 120 VTSQKCVRAG GKHNDLENVG YTARHHTFFE MLGNFSFGDY FKEEAIRLAW NFLTVELGLP 130 140 150 160 170 180 AEKMWVSVFE DDDEAFALWE KVEDLPKGRI VRLGEKDNFW AMGDTGPCGP CSEIHIDQGV 190 200 210 220 230 240 GSSPCDNPNC AVGCDCDRFL ELWNLVFMQF NRAEDGSLTA LPRPSIDTGM GLERVAAVLQ 250 260 270 280 290 300 GKFNNYDSDL FAPIIAVLED ISGVKYGAAA DTDTAIRVIA DHARATSFLV ADGVLPSNEG 310 320 330 340 350 360 RGYVLRRIMR RAVRYGKKLG LEKPFMDRVT KAVCAEMQNA YPQLVATAAL LEKVVNNEEE 370 380 390 400 410 420 RFRETLEHGL VQLDEKISQL LTSGGDAVID GPFIFKLYDT FGFPFDIVRD IALERGVGFD 430 440 450 460 470 480 EAGFATAMAE QRAKSRASRK GEGVKLHDEG VKALADAGKK AEFLGYEGLE ADSVVEGLLS 490 500 510 520 530 540 EQGSGVEKLV AGEKGRVFVA ATPFYAEAGG QMGDRGSVRW QGGQASVYAT QAEGTGLILH 550 560 570 580 590 600 DLLVEEGELS LGLEVTLQVD DEERKATASN HSATHLLQAA LISVLGDHVK QSGSLVGPER 610 620 630 640 650 660 LRFDFTNFSQ LTAAEIAQVE TLVNEQIRNN AVIATDVLSK QEAIAGGATA LFGEKYDDDV 670 680 690 700 710 720 RVVSMGDYSR ELCGGTHVGA TGEIGLFVIL SESGIAAGVR RIEALTGRAA LAYVQGRLST 730 740 750 760 770 780 GNELADLLSC KSGDLVPKVE SLLTAVKEGE KRVAQLAGQL ASSGLDDLLN NALTVAGIKV 790 800 810 820 830 840 VVAEVPLENA KALRELGDKV RDNLESGIAV IGGAVGGKVA LLAIVTKDLV DRIQAGRIVS 850 860 870 880 EVSGIVGGKG GGRPDMAQAG GTMPDKLSEA IASVPAIIEA ML

References

[1]

"The genome of Desulfotalea psychrophila, a sulfate-reducing bacterium from permanently cold Arctic sediments." Rabus R., Ruepp A., Frickey T., Rattei T., Fartmann B., Stark M., Bauer M., Zibat A., Lombardot T., Becker I., Amann J., Gellner K., Teeling H., Leuschner W.D., Gloeckner F.-O., Lupas A.N., Amann R., Klenk H.-P. Environ. Microbiol. 6:887-902(2004) [PubMed: 15305914] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: LSv54 / DSM 12343.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR522870 Genomic DNA. Translation: CAG35557.1.
RefSeq	YP_064564.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2944938.
GenomeReviews	Gene locus DP0828 in contig CR522870_GR.
KEGG	dps:DP0828.
NMPDR	fig|177439.1.peg.828.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6AQ16.
Enzyme and pathway databases
BioCyc	DPSY177439:DP0828-MON.
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR003156. Pesterase_DHHA1. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProDom	Q6AQ16. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Entry information

Entry name

SYA_DESPS

Accession

Primary (citable) accession number: Q6AQ16

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	September 13, 2004
Last modified:	July 22, 2008
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)